| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,823,545 | C→T | 10.2% | intergenic (+290/+668) | nrfG → / ← pheU | heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfG/tRNA‑Phe |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,823,545 | 0 | C | T | 10.2% | 394.0 / 27.3 | 127 | intergenic (+290/+668) | nrfG/pheU | heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfG/tRNA‑Phe |
| Reads supporting (aligned to +/- strand): ref base C (59/55); new base T (4/9); total (63/64) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.41e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
Only 100 of 130 total aligned reads displayed.
GATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGAAA > minE/2823475‑2823615 | gATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCAc > 1:4058435/1‑71 (MQ=255) tACTGATTATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGt < 1:1916114/71‑1 (MQ=255) tACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGt < 1:3499193/71‑1 (MQ=255) tACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGt < 1:3503747/71‑1 (MQ=255) aCTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGtt > 1:3671552/1‑71 (MQ=255) cTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTtc < 1:2027349/71‑1 (MQ=255) cTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTtc < 1:432796/71‑1 (MQ=255) gATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTtctc > 1:2354456/1‑71 (MQ=255) gATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTtctc > 1:2214661/1‑71 (MQ=255) gATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTtctc > 1:3427788/1‑71 (MQ=255) gATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTtctc > 1:3529516/1‑71 (MQ=255) gATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTtctc > 1:3763905/1‑71 (MQ=255) aTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTcg < 1:2538196/71‑1 (MQ=255) cATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTcgcg > 1:1644123/1‑71 (MQ=255) cATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTcgcg > 1:3308283/1‑71 (MQ=255) tAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGtt < 1:633314/71‑1 (MQ=255) aaGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGttt > 1:3268138/1‑71 (MQ=255) aGCGTAAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTc > 1:3628114/1‑71 (MQ=255) gCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTtct > 1:3774409/1‑62 (MQ=255) gCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTtct > 1:3047985/1‑62 (MQ=255) cGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTg < 1:3752381/71‑1 (MQ=255) cGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTg < 1:3897787/71‑1 (MQ=255) tAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTg < 1:1544242/68‑1 (MQ=255) aaaaaaaTCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAAc < 1:3978730/70‑1 (MQ=255) aaaaaaaTCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAAc < 1:2174451/70‑1 (MQ=255) aaaaaaaTCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACg > 1:4055124/1‑71 (MQ=255) aaaaaaaTCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACg > 1:2100090/1‑71 (MQ=255) aaaaaaaTCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACg > 1:3147156/1‑71 (MQ=255) aaaaaTCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACggg > 1:210522/1‑71 (MQ=255) aaaaaTCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACgg > 1:822238/1‑70 (MQ=255) aaaaTCTACAAGCCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACggg > 1:266021/1‑70 (MQ=255) aaaaTCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCATGTTCTCGCGTTTCTg < 1:1603401/64‑1 (MQ=255) aaaaTCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACgggg < 1:218033/71‑1 (MQ=255) aaaaTCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACgggg < 1:2921417/71‑1 (MQ=255) aaaaTCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACgggg < 1:2758953/71‑1 (MQ=255) aaaTCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACgggg > 1:760620/1‑70 (MQ=255) aaaTCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACgggg < 1:622080/70‑1 (MQ=255) aaTCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGaa < 1:976160/71‑1 (MQ=255) aTCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAAc < 1:2621571/71‑1 (MQ=255) tACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACggg > 1:855253/1‑64 (MQ=255) tACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACgg > 1:955528/1‑63 (MQ=255) cAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCATGTTCTCGCGTTTCTGAACGGGGAACGgcgc > 1:738761/1‑71 (MQ=255) cAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGt > 1:1715858/1‑51 (MQ=255) cAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGgcgc > 1:551641/1‑71 (MQ=255) cAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGgcg > 1:3166126/1‑70 (MQ=255) aaaCCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCt > 1:2612330/1‑71 (MQ=255) aaCCAACGCAACACAATTCATGCCCTGGCAGTATGTCATGATCTCGCGTTTCTGAACGGGGAACGGCGCTc < 1:2510709/71‑1 (MQ=255) aaCCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTc < 1:1490552/71‑1 (MQ=255) aaCCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTc < 1:4016135/71‑1 (MQ=255) aCCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACgggg > 1:1018360/1‑60 (MQ=255) aCCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTc > 1:2470254/1‑70 (MQ=255) aCCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTc > 1:3501839/1‑70 (MQ=255) aCCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTc > 1:1986461/1‑70 (MQ=255) aCCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTc > 1:3591768/1‑70 (MQ=255) aCCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTc > 1:1081709/1‑70 (MQ=255) aCCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGCGGAACGGCGCTc > 1:3729594/1‑70 (MQ=255) ccAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCa < 1:1264433/71‑1 (MQ=255) ccAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCa < 1:3009886/71‑1 (MQ=255) ccAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCa < 1:1117315/71‑1 (MQ=255) ccAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCa < 1:1002968/71‑1 (MQ=255) aaCGCAACACAATTCATGCCCTGGCAGTATGTCATGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCAtt < 1:4121494/71‑1 (MQ=255) aaCGCAACACAATTCATGCCCTGGCAGTATGTCATGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCAtt < 1:2559555/71‑1 (MQ=255) aaCGCAACACAATTCATGCCCTGGCAGTATGTCATGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCAtt < 1:2670042/71‑1 (MQ=255) aaCGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCAtt < 1:2541755/71‑1 (MQ=255) aaCGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCAtt < 1:2584250/71‑1 (MQ=255) cAACACAATTCATGCCCTGGCAGTATGTCATGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAgg < 1:3242920/71‑1 (MQ=255) cAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAgg < 1:1210074/71‑1 (MQ=255) aaCACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAgg < 1:2785468/70‑1 (MQ=255) acaATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGt < 1:782513/71‑1 (MQ=255) caATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGt > 1:311703/1‑70 (MQ=255) tCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGtca > 1:3845481/1‑68 (MQ=255) tCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGtca > 1:2107352/1‑68 (MQ=255) aTGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGtcattca < 1:170640/70‑1 (MQ=255) tGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGtcattcat > 1:738175/1‑70 (MQ=255) gCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGaa > 1:2845424/1‑35 (MQ=255) gCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGaa > 1:801575/1‑35 (MQ=255) ccTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCataata < 1:177133/71‑1 (MQ=255) cTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCataata < 1:3866043/70‑1 (MQ=255) cTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCataa > 1:1429523/1‑68 (MQ=255) tGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCataa < 1:3511102/67‑1 (MQ=255) tGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAg > 1:2297445/1‑70 (MQ=255) tGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAg > 1:3861540/1‑70 (MQ=255) gCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCataata < 1:2293055/67‑1 (MQ=255) gCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGa < 1:1510578/69‑1 (MQ=255) cAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAAc < 1:1621662/71‑1 (MQ=255) tATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAg < 1:2690303/64‑1 (MQ=255) aTGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCAt > 1:453458/1‑71 (MQ=255) aTGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCAt > 1:159237/1‑71 (MQ=255) tGTCATGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCAtt > 1:1055872/1‑71 (MQ=255) tGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGa < 1:3895740/63‑1 (MQ=255) tGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGa < 1:2134919/63‑1 (MQ=255) tCATGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGaaa > 1:1659133/1‑63 (MQ=255) tCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCa < 1:3669158/38‑1 (MQ=255) tCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCg < 1:2510198/71‑1 (MQ=255) tCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCg < 1:2993098/71‑1 (MQ=255) tCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCg < 1:893090/71‑1 (MQ=255) tCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCg < 1:3934699/71‑1 (MQ=255) aCGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGaa < 1:1659651/71‑1 (MQ=255) cGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCataata < 1:2818764/56‑1 (MQ=255) cGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGaaa < 1:1869328/71‑1 (MQ=255) | GATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGAAA > minE/2823475‑2823615 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |