| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,826,736 | G→A | 9.3% | P95S (CCT→TCT) | cutA ← | copper binding protein, copper sensitivity |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,826,736 | 0 | G | A | 9.3% | 301.5 / 16.4 | 97 | P95S (CCT→TCT) | cutA | copper binding protein, copper sensitivity |
| Reads supporting (aligned to +/- strand): ref base G (38/50); new base A (4/5); total (42/55) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01 | |||||||||||
Only 100 of 110 total aligned reads displayed.
GCAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGA > minE/2826666‑2826806 | gcAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAgg > 1:1041443/1‑71 (MQ=255)gcAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAgg > 1:2324264/1‑71 (MQ=255)gcAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAgg > 1:1212008/1‑71 (MQ=255) cAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAgg > 1:922625/1‑70 (MQ=255) cAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAgg > 1:2585909/1‑70 (MQ=255) cAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAgg < 1:1861502/70‑1 (MQ=255) cAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAgg < 1:3802365/70‑1 (MQ=255) aaaGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGt > 1:3374379/1‑70 (MQ=255) aaGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGATGTAATCTGTGTCTCCGTGTGTAACAGGTaa < 1:2179977/71‑1 (MQ=255) aaGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTaa < 1:3720901/71‑1 (MQ=255) aaGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTaa < 1:3145118/71‑1 (MQ=255) aaGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTa > 1:3919694/1‑70 (MQ=255) aGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTaa > 1:2164491/1‑70 (MQ=255) aGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTaa > 1:2266686/1‑70 (MQ=255) gTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTaaaa > 1:3567560/1‑71 (MQ=255) gTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGATaa < 1:1493917/69‑1 (MQ=255) gTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGATaa < 1:3915530/69‑1 (MQ=255) tAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAAc > 1:560750/1‑71 (MQ=255) tAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAAc > 1:1903904/1‑71 (MQ=255) cAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAg < 1:1201714/71‑1 (MQ=255) tCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGtt > 1:3454358/1‑71 (MQ=255) aGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTcc < 1:421988/71‑1 (MQ=255) gCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCg < 1:3429421/71‑1 (MQ=255) gCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCg < 1:1741910/71‑1 (MQ=255) cGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCgg > 1:2109430/1‑71 (MQ=255) gTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCggg > 1:827155/1‑71 (MQ=255) tAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGa < 1:1256400/61‑1 (MQ=255) tAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCgggg > 1:4106869/1‑71 (MQ=255) aaaGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGt > 1:647431/1‑71 (MQ=255) aaGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGtt > 1:3383736/1‑71 (MQ=255) aaGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGtt > 1:3685400/1‑71 (MQ=255) aaGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGtt > 1:947044/1‑71 (MQ=255) aGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCAGTGTGTAACAGATAAAACCAGAAGTTCCGGGGttt < 1:495479/71‑1 (MQ=255) gATTCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGttt > 1:3583551/1‑70 (MQ=255) gATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTg < 1:1591948/71‑1 (MQ=255) gATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTg < 1:440693/71‑1 (MQ=255) gATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTg < 1:1273289/71‑1 (MQ=255) aTGCGTTGAGCCATGAGATGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCa < 1:140733/54‑1 (MQ=255) tGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGa < 1:1014667/70‑1 (MQ=255) gCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGttt > 1:1894582/1‑67 (MQ=255) gCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGttt > 1:1889562/1‑67 (MQ=255) cGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGata < 1:1554260/70‑1 (MQ=255) gTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATg < 1:2189862/71‑1 (MQ=255) ttGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGa < 1:3376335/51‑1 (MQ=255) ttGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTcc < 1:1217492/57‑1 (MQ=255) tGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTATCAGGTAAAACCAGa < 1:3733963/50‑1 (MQ=255) tGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGga > 1:3077568/1‑71 (MQ=255) tGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGATAAAACCAGAAGTTCCGGGGTTTGATATGga > 1:3107274/1‑71 (MQ=255) gAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTaa > 1:1127709/1‑42 (MQ=255) gAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTaa > 1:2460453/1‑42 (MQ=255) gAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGata > 1:2600621/1‑66 (MQ=255) gAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGgat > 1:2987069/1‑71 (MQ=255) aGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGgatg < 1:628174/71‑1 (MQ=255) gCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGgatg < 1:2756184/70‑1 (MQ=255) ccATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTaaa < 1:265552/40‑1 (MQ=255) aTGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTaaaa > 1:2615847/1‑39 (MQ=255) aTGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGt > 1:75100/1‑55 (MQ=255) aTGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGgatgat > 1:703671/1‑69 (MQ=255) tGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGt < 1:730694/54‑1 (MQ=255) gagaGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGttt < 1:2168731/55‑1 (MQ=255) agagGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGAc > 1:3442868/1‑71 (MQ=255) gagGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGAtgatga < 1:2160173/67‑1 (MQ=255) gagGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGAtgatga < 1:3821391/67‑1 (MQ=255) agGTAATCTGTGTCTCCGTGTGTAACAGATAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACtt > 1:1547854/1‑71 (MQ=255) aTCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAgg < 1:337744/70‑1 (MQ=255) aTCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAgg < 1:464356/70‑1 (MQ=255) aTCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAgg < 1:2550373/70‑1 (MQ=255) aTCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGCGTTTGata > 1:2187010/1‑51 (MQ=255) aTCTGTGTCTCCGTGTGTAACAGATAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAgg > 1:1840555/1‑70 (MQ=255) tCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGga < 1:846995/54‑1 (MQ=255) tgtgTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCAtt > 1:912810/1‑71 (MQ=255) tgtgTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCAtt > 1:3280280/1‑71 (MQ=255) tgtCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCg < 1:3896805/36‑1 (MQ=255) tgtCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCg < 1:3840279/36‑1 (MQ=255) tgtCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCAtt < 1:924771/69‑1 (MQ=255) tgtCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTcc < 1:2648890/71‑1 (MQ=255) tgtCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTc < 1:926481/70‑1 (MQ=255) tgtCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTc < 1:1498838/70‑1 (MQ=255) tgtCTCCGTGTGTAACAGATAAAACCAGAAGTTCCg < 1:2792588/36‑1 (MQ=255) tctcCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCcag < 1:3857954/71‑1 (MQ=255) tcCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATgg < 1:234538/45‑1 (MQ=255) tcCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATgg < 1:1971763/45‑1 (MQ=255) cGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCcagca < 1:1618425/69‑1 (MQ=255) cGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCcagca < 1:301938/69‑1 (MQ=255) cGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCcagca < 1:258826/69‑1 (MQ=255) cGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGt < 1:1624360/71‑1 (MQ=255) gtgtgtAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATg > 1:1729309/1‑41 (MQ=255) gtgtgtAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATg > 1:928507/1‑41 (MQ=255) gtgtgtAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGga > 1:2944410/1‑43 (MQ=255) tAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGt < 1:1763193/65‑1 (MQ=255) tAACAGATAAAACCAGAAGTTCCGGGGTTTCATATGGATGATgag < 1:1099966/45‑1 (MQ=255) aCAGGTAAAACCAGAAGTTCCGGGGTTTGATATGgat > 1:3003813/1‑37 (MQ=255) aCAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCa > 1:2372716/1‑49 (MQ=255) aCAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCctgctg < 1:3501080/71‑1 (MQ=255) aGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGt > 1:2858566/1‑71 (MQ=255) aGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGt < 1:2131088/71‑1 (MQ=255) ggTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAgg < 1:696295/48‑1 (MQ=255) ggTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTgg > 1:3037947/1‑69 (MQ=255) gTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGt < 1:319544/59‑1 (MQ=255) gTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTga < 1:1936047/71‑1 (MQ=255) | GCAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGA > minE/2826666‑2826806 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |