| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,861,362 | A→G | 22.2% | Q150R (CAG→CGG) | miaA → | delta(2)‑isopentenylpyrophosphate tRNA‑adenosine transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,861,362 | 0 | A | G | 22.2% | 124.7 / 36.5 | 63 | Q150R (CAG→CGG) | miaA | delta(2)‑isopentenylpyrophosphate tRNA‑adenosine transferase |
| Reads supporting (aligned to +/- strand): ref base A (19/30); new base G (3/11); total (22/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.43e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
GGAAGTACGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGG > minE/2861294‑2861430 | ggAAGTACGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCagg < 1:3579808/71‑1 (MQ=255)ggAAGTACGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCagg < 1:2083939/71‑1 (MQ=255)ggAAGTACGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCag > 1:1716106/1‑70 (MQ=255) gTACGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGt < 1:1277356/71‑1 (MQ=255) cGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTGATTGCATCGTCAACTTCgggagg > 1:1794981/1‑67 (MQ=255) cGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCaggagg > 1:648609/1‑67 (MQ=255) cGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCaggagg > 1:3649504/1‑67 (MQ=255) cGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCaggagg > 1:3775806/1‑67 (MQ=255) gggCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCGGGAGGTAGAt < 1:1353039/71‑1 (MQ=255) gggCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCGGGAGGTAGAt < 1:2854110/71‑1 (MQ=255) ggCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATc < 1:630735/71‑1 (MQ=255) ccAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCg < 1:2323232/71‑1 (MQ=255) aTTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGc > 1:837195/1‑71 (MQ=255) aTTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGc < 1:691062/71‑1 (MQ=255) aTTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGc < 1:600664/71‑1 (MQ=255) aTTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGc < 1:454289/71‑1 (MQ=255) aTTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGc < 1:3969568/71‑1 (MQ=255) ttGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCg > 1:850812/1‑71 (MQ=255) tGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCgg > 1:1591809/1‑71 (MQ=255) cAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCGGGAGGTAGATCCGGTTGCGgcag > 1:914265/1‑70 (MQ=255) cAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCGGGAGGTAGATCCGGTTGCGgcag > 1:103596/1‑70 (MQ=255) cAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGgcagc < 1:2722027/71‑1 (MQ=255) cAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAg > 1:3402350/1‑71 (MQ=255) ggcggcAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGa > 1:932752/1‑71 (MQ=255) gcggcAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGAt < 1:3415336/71‑1 (MQ=255) ggcAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCGGGAGGTAGATCCGGTTGCGGCAGCAAGGATTc < 1:3264685/71‑1 (MQ=255) ggcAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCGGGAGGTAGATCCGGTTGCGGCAGCAAGGATTc < 1:2161522/71‑1 (MQ=255) ggcAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCGGGAGGTAGATCCGGTTGCGGCAGCAAGGATTc < 1:1915406/71‑1 (MQ=255) ggcAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTc < 1:2223624/71‑1 (MQ=255) agagCAAGGTTGGGAGTCATTGCATCGTCAACTTCagg > 1:1013446/1‑38 (MQ=255) agagCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAg > 1:3277016/1‑44 (MQ=255) agagCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAg > 1:2390507/1‑44 (MQ=255) agagCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAg > 1:2562661/1‑44 (MQ=255) agagCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAg > 1:2994432/1‑44 (MQ=255) agagCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAg > 1:2896221/1‑44 (MQ=255) agagCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAg > 1:1527162/1‑44 (MQ=255) agCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCgg < 1:3017098/54‑1 (MQ=255) agCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCgg < 1:251947/54‑1 (MQ=255) agCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCa < 1:43106/71‑1 (MQ=255) gCAAGGTTGGGAGTCATTGCATCGTCAACTTCGGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCaa < 1:1060735/71‑1 (MQ=255) gCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCaa < 1:672493/71‑1 (MQ=255) gCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCaa < 1:484660/71‑1 (MQ=255) gCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCaa < 1:2629316/71‑1 (MQ=255) gCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCaa < 1:2643953/71‑1 (MQ=255) cAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATc > 1:2001740/1‑67 (MQ=255) cAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCaaa > 1:1018449/1‑71 (MQ=255) cAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCaa < 1:4112246/70‑1 (MQ=255) aGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATg < 1:485645/71‑1 (MQ=255) tGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATc > 1:2726115/1‑70 (MQ=255) gAGTCATTGCATCGTCAACTTCGGGAGGTAGATCCGGTTGCGGCAGCaa < 1:2719824/49‑1 (MQ=255) gAGTCATTGCATCGTCAACTTCGGGAGGTAGATCCGGTTGCGGCAGCaa < 1:526457/49‑1 (MQ=255) gAGTCATTGCATCGTCAACTTCGGGAGGTAGATCCGGTTGCGGCAGCaa < 1:2861901/49‑1 (MQ=255) aGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCcaca > 1:1725558/1‑70 (MQ=255) tCATTGCATCGTCAACTTCGGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAg > 1:3704579/1‑71 (MQ=255) tCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCa < 1:391864/57‑1 (MQ=255) tCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACaaa > 1:891472/1‑70 (MQ=255) tCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAg < 1:3538169/71‑1 (MQ=255) tCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAg < 1:1769537/71‑1 (MQ=255) tCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAg > 1:3088215/1‑71 (MQ=255) aTTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAg < 1:3500415/45‑1 (MQ=255) ttGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCcac < 1:2262037/64‑1 (MQ=255) gCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCttt > 1:66218/1‑71 (MQ=255) cATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTc < 1:1382317/71‑1 (MQ=255) aTCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTcc < 1:3623262/71‑1 (MQ=255) tCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACaa < 1:809820/61‑1 (MQ=255) tCAACTTCGGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCa < 1:3302085/46‑1 (MQ=255) tCAACTTCGGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCa < 1:461734/46‑1 (MQ=255) cAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTgg < 1:818426/70‑1 (MQ=255) cAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTgg > 1:2560073/1‑70 (MQ=255) cAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTg < 1:725245/69‑1 (MQ=255) cAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTg < 1:3355850/69‑1 (MQ=255) | GGAAGTACGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGG > minE/2861294‑2861430 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |