| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,882,226 | G→A | 15.4% | E24E (GAG→GAA) | ytfH → | predicted transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,882,226 | 0 | G | A | 15.4% | 281.0 / 34.0 | 104 | E24E (GAG→GAA) | ytfH | predicted transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (39/49); new base A (11/5); total (50/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.02e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 108 total aligned reads displayed.
TGAGTCAGGTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGTTGGGGGGTGTTGATTCTGGTGGCGCTACGCGAAGGTACTCATCGC > minE/2882156‑2882295 | tGAGTCAGGTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAg > 1:2230263/1‑71 (MQ=255) gAGTCAGGTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAgg > 1:915062/1‑71 (MQ=255) gAGTCAGGTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAgg > 1:769810/1‑71 (MQ=255) gAGTCAGGTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAgg > 1:222475/1‑71 (MQ=255) tCAGGTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGgtgt < 1:1573809/71‑1 (MQ=255) tCAGGTTAGCATGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGgtgt < 1:2397246/71‑1 (MQ=255) cAGGTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGtt > 1:199204/1‑71 (MQ=255) aGGTTAGCCTGTTGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGtt > 1:1213248/1‑70 (MQ=255) ggTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGa < 1:2721755/71‑1 (MQ=255) ggTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGa < 1:3520211/71‑1 (MQ=255) ggTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGa < 1:280441/71‑1 (MQ=255) ggTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAAGTGTTGa < 1:403751/71‑1 (MQ=255) gTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGaa < 1:3932052/71‑1 (MQ=255) gTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGa > 1:1983750/1‑70 (MQ=255) ttAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGCGCCCGTCGCGCGAGGTGTTGaaa < 1:3946189/71‑1 (MQ=255) tAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGaaa > 1:2724786/1‑70 (MQ=255) tAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTAGCGGAACAGTGCCCGTCGCGCGAGGTGTTGaaa > 1:1601838/1‑70 (MQ=255) aGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAac < 1:1809470/70‑1 (MQ=255) aGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAac < 1:2294116/70‑1 (MQ=255) aGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAac < 1:3429861/70‑1 (MQ=255) gCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAacac < 1:1311167/71‑1 (MQ=255) gCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAacac < 1:1306409/71‑1 (MQ=255) ccTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACg < 1:2435045/71‑1 (MQ=255) ccTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACg < 1:2998410/71‑1 (MQ=255) ccTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACg < 1:2439748/71‑1 (MQ=255) tGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAAGTGTTGa > 1:17557/1‑63 (MQ=255) tGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTAGCGCGAAGTGTTGa > 1:1712005/1‑63 (MQ=255) tCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTc < 1:2044648/69‑1 (MQ=255) tCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCAc > 1:1161950/1‑71 (MQ=255) tCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCAc > 1:252743/1‑71 (MQ=255) tCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAAGTGTTGAAACACGTc < 1:2374239/69‑1 (MQ=255) tCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAAGTGTTGAAACACGTCAc > 1:4016478/1‑71 (MQ=255) gCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGaa > 1:2533915/1‑60 (MQ=255) gCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCAcc < 1:226283/70‑1 (MQ=255) gCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCAc > 1:2137151/1‑69 (MQ=255) acaacTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAaca > 1:2947244/1‑60 (MQ=255) acaacTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAaca > 1:3976611/1‑60 (MQ=255) caacTTAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCAcc > 1:1109354/1‑66 (MQ=255) caacTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAacac < 1:1534044/60‑1 (MQ=255) caacTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCg > 1:1118038/1‑71 (MQ=255) caacTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAAGTGTTGAAACACGTCACCAGCCg > 1:2434044/1‑71 (MQ=255) aacTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGaaa > 1:1982128/1‑56 (MQ=255) acTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGtt < 1:3497134/71‑1 (MQ=255) acTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGtt < 1:1327855/71‑1 (MQ=255) aaaGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGTTggg > 1:3908930/1‑70 (MQ=255) aaaGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGTTggg < 1:1068783/70‑1 (MQ=255) aaaGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGTTggg > 1:165529/1‑70 (MQ=255) gagGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGt < 1:680910/63‑1 (MQ=255) gagGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGTTGGGGGgt < 1:3098422/71‑1 (MQ=255) agGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGTTGGGGGgtg > 1:1227180/1‑71 (MQ=255) tAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGc < 1:489356/55‑1 (MQ=255) tAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGtt < 1:1196566/59‑1 (MQ=255) tAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGt < 1:3374685/58‑1 (MQ=255) tAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGt < 1:3592628/58‑1 (MQ=255) tAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGTTGGGGGGTGTTGa < 1:404874/71‑1 (MQ=255) aaTCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGc < 1:1995353/54‑1 (MQ=255) aTCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGt > 1:146697/1‑56 (MQ=255) aTCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGTTgg > 1:1699372/1‑59 (MQ=255) aTCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGTTGGGGGGTGTTGAtt < 1:4082539/71‑1 (MQ=255) tctcTTTGCGGAACAGTGCCCGTCGCGCGAGgtgt > 1:1135250/1‑35 (MQ=255) tctcTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGaaa > 1:2107512/1‑40 (MQ=255) tctcTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGaaa > 1:2358158/1‑40 (MQ=255) ctctTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGaaa > 1:898467/1‑39 (MQ=255) ctctTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGaaa > 1:3012081/1‑39 (MQ=255) ctctTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGaaa > 1:1180934/1‑39 (MQ=255) tctTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGaaa > 1:671144/1‑38 (MQ=255) tctTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGa < 1:234502/36‑1 (MQ=255) tctTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGt < 1:3815246/53‑1 (MQ=255) tctTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGt < 1:2820566/53‑1 (MQ=255) tctTTGCGGAACAGTGCCCGTCGCGCGAAGTGTTGAAacac > 1:3203789/1‑41 (MQ=255) tctTTGCGGAACAGTGCCCGTCGCGCGAAGTGTTGAAaca > 1:2056708/1‑40 (MQ=255) tctTTGCGGAACAGTGCCCGTCGCGCGAAGTGTTGAAACACGTCACCAGCCGt > 1:823981/1‑53 (MQ=255) tctTTGCGGAACAGTGCCCGTCGCGCGAAGTGTTGAAACACGTCACCAGCCGt > 1:2410300/1‑53 (MQ=255) ctTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGtt < 1:685303/53‑1 (MQ=255) ctTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGtt < 1:236753/53‑1 (MQ=255) ctTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGtt < 1:2609206/53‑1 (MQ=255) ctTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGtt < 1:2649540/53‑1 (MQ=255) ctTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGtt < 1:1854781/53‑1 (MQ=255) tttGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGTTGGGGGGTGTTGATTCt < 1:2167992/68‑1 (MQ=255) tttGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGTTGGGGGGTGTCGATTCt < 1:2664542/68‑1 (MQ=255) ttGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGTTGGGGGGTGTTGa < 1:2036298/63‑1 (MQ=255) ttGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGCCACCAGCCGTTgg > 1:3207364/1‑53 (MQ=255) tGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGTTGGGGGGTGTTGATTCtggt < 1:2953137/69‑1 (MQ=255) gCGGAACAGTGCCCGTCGCGCGAAGTGTTGAAACACGTCACCAGCCGTTGGGGGGTGTTGATTCTGGTGgc > 1:102222/1‑71 (MQ=255) aaCAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGTTGGGGGGTGTTGATTCTGGTGGCGCTa > 1:3659178/1‑71 (MQ=255) aCAGTGCCCGTCGCGCGAAGTGTTGAAACACGTCACCAGCCGTTGGGGGGTGTTGATTCTGGTGGCGCTAc < 1:3571563/71‑1 (MQ=255) gTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGTTGGGGGGTGTTGATTCTGGTGGCGCTAcgcg < 1:3143420/71‑1 (MQ=255) gTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGTTGGGGGGTGTTGATTCTGGTGGCGCTAcgcg < 1:4109670/71‑1 (MQ=255) gTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGTTGGGGGGTGTTGATTCTGGTGGCGCTAcgc > 1:3013116/1‑70 (MQ=255) gTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGTTGGGGGGTGTTGATTCTGGTGGCGCTAcgc > 1:3545879/1‑70 (MQ=255) gTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGTTGGGGGGTGTTGATTCTGGTGGCGCTAcgc > 1:725039/1‑70 (MQ=255) gTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGTTGGGGGGTGTTGATTCTGGTGGCGCTAcgc > 1:3861820/1‑70 (MQ=255) cccGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGTTGGGGGGTGTTGATTCTGGTGGCGCTACGCGAAg > 1:808184/1‑71 (MQ=255) cccGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGTTGGGGGGTGTTGATTATGGTGGCGCTACGCGAAg > 1:2823350/1‑71 (MQ=255) ccGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGTTGGGGGGTGTTGATTCTGGTGGCGCTACGCGAAgg > 1:68247/1‑71 (MQ=255) ccGTCGCGCGAAGTGTTGAAACACGTCACCAGCCGTTGGGGGGTGTTGATTCTGGTGGCGCTACGCGAAgg < 1:3810882/71‑1 (MQ=255) gcgcgAGGTGTTGAAACACGTCACCAGCCGTTGGGGGGTGTTGATTCTGGTGGCGCTACGCGAAGGTACt > 1:1610662/1‑70 (MQ=255) gcgcgAGGTGTTGAAACACGTCACCAGCCGTTGGGGGGTGTTGATTCTGGTGGCGCTACGCGAAGGTACt > 1:851061/1‑70 (MQ=255) gcgcgAGGTGTTGAAACACGTCACCAGCCGTTGGGGGGTGTTGATTCTGGTGGCGCTACGCGAAGGTACTc < 1:1812895/71‑1 (MQ=255) aGGTGTTGAAACACGTCACCAGCCGTTGGGGGGTGTTGATTCTGGTGGCGCTACGCGAAGGTACTCATCGc < 1:738554/71‑1 (MQ=255) | TGAGTCAGGTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGTTGGGGGGTGTTGATTCTGGTGGCGCTACGCGAAGGTACTCATCGC > minE/2882156‑2882295 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |