Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,359,876	C→T	42.9%	noncoding (1219/2905 nt)	rrlA ←	23S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,359,876	0	C	T	42.9%	7.9 / 23.1	21	noncoding (1219/2905 nt)	rrlA	23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base C (10/2);  new base T (4/5);  total (14/7)
Fisher's exact test for biased strand distribution p-value = 1.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGC  >  minE/2359823‑2359935                                                      |                                                            aTCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTa                                            >  1:181091/1‑71 (MQ=34)    gTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAg                                         <  1:179007/71‑1 (MQ=255)       aCTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAAc                                      >  1:341756/1‑71 (MQ=34)          tATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCt                                   >  1:70113/1‑71 (MQ=34)           aTGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTc                                  >  1:147979/1‑71 (MQ=34)           aTGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTc                                  >  1:355170/1‑71 (MQ=34)           aTGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGc                                    <  1:217680/69‑1 (MQ=255)           aTGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTc                                  >  1:296676/1‑71 (MQ=255)           aTGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTc                                  >  1:155491/1‑71 (MQ=255)              tCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTcccc                               <  1:431366/71‑1 (MQ=11)              tCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTccc                                <  1:417954/70‑1 (MQ=255)                aGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCt                              >  1:41886/1‑70 (MQ=35)                   aTTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTAcc                           >  1:155361/1‑70 (MQ=255)                       gCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaaca                      <  1:16949/71‑1 (MQ=255)                            tCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTcccc                               >  1:106781/1‑57 (MQ=35)                             cTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTAccc                          >  1:115077/1‑61 (MQ=35)                             cTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTAc                            >  1:411074/1‑59 (MQ=35)                                 tACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAgtg            >  1:19492/1‑71 (MQ=255)                                      ccAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAacaaca                   <  1:395830/59‑1 (MQ=38)                                          cATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTAc                            >  1:215772/1‑46 (MQ=11)                                           aTGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGc  <  1:31064/71‑1 (MQ=255)                                                      |                                                            ATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGC  >  minE/2359823‑2359935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑