| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 486,916 | A→G | intergenic (+72/‑93) | ybgF → / → lysT | hypothetical protein/tRNA‑Lys |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 486,916 | 0 | A | G | 99.7% | 1020.8 / ‑6.2 | 349 | intergenic (+72/‑93) | ybgF/lysT | hypothetical protein/tRNA‑Lys |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (347/0); total (349/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
Only 100 of 349 total aligned reads displayed.
TGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCG > minE/486906‑486967 | tGAGTGATCTTCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:611657/1‑62 (MQ=255)tGAGTGATCTGCATCGAATTTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATgccgcag > 1:242176/1‑60 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAg > 1:815961/1‑47 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAatatat > 1:1117790/1‑55 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAatatat > 1:597748/1‑55 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATgccgcc > 1:772497/1‑61 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATgccgcc > 1:195306/1‑61 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATgccgcc > 1:863544/1‑61 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATgcc > 1:375562/1‑58 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATgc > 1:576232/1‑57 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATg > 1:340802/1‑56 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:679569/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:647220/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:671142/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:735900/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:690699/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:703611/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:723995/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:494603/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:640708/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:624318/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:592091/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:583286/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:567429/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:55588/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:540161/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:5264/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:520525/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:509497/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:451015/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:877434/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:997514/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:987977/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:973502/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:964555/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:949880/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:926945/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:909501/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:904578/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:900102/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:892975/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:891154/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:744455/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:849681/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:842280/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:835339/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:826184/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:824458/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:821566/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:803167/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:796520/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:784080/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:761634/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:116450/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:231899/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:205877/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:1857/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:181020/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:163698/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:156800/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:150616/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:132567/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:129193/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:123506/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:117698/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:235479/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:1133829/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:1123646/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:1107962/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:1101369/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:1093731/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:1084092/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:1062591/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:1048032/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:1046534/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:1032303/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:1026018/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:352450/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:457417/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:1011208/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:443151/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:442029/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:436249/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:42329/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:414597/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:405266/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:390969/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:381150/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:358514/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:481959/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:346244/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:320910/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:315218/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:306679/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:299323/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:292786/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:289428/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:279652/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:273296/1‑62 (MQ=255)tGAGTGATCTGCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGccgccg > 1:26122/1‑62 (MQ=255) | TGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCG > minE/486906‑486967 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |