Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 2,565,280 G→T R62R (CGG→CGT)  yibT → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,565,2800GT99.8% 1805.4 / ‑6.0 562R62R (CGG→CGT) yibThypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (561/0);  total (562/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 562 total aligned reads displayed.

CGGCTTTATCGGCCGAAGCAGGTAGAGGGGCAGTAAGCAGACGTTGCTTACTTTTGAAAGGCTT  >  minE/2565269‑2565332
           |                                                    
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTATGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:222961/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAgg     >  1:234206/3‑60 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGc    >  1:1007711/3‑61 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGc    >  1:827567/3‑61 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGc    >  1:590248/3‑61 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGc    >  1:578258/3‑61 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGc    >  1:254466/3‑61 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGc    >  1:551108/3‑61 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGc    >  1:961440/3‑61 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:702547/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:711342/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:720898/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:517497/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:691187/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:680192/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:669194/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:732439/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:65246/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:645797/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:637413/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:624465/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:616236/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:611477/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:60166/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:56487/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:560026/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:539608/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:529710/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:1012573/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:863204/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:98199/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:97680/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:967695/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:949454/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:942130/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:936566/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:920342/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:915196/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:899703/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:879496/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:871106/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:734553/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:855336/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:850811/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:83925/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:819973/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:810777/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:789020/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:780129/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:76600/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:760134/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:750688/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:1128313/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:268421/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:260257/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:246278/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:19432/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:184592/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:176791/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:169882/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:157319/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:147761/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:130765/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:115735/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:488885/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:1119399/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:1109590/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:1103944/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:1096540/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:1089770/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:1085284/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:1070524/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:1066697/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:1058498/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:1034651/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:1024840/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:281251/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:503682/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:481718/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:465458/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:446452/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:431939/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:423213/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:414956/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:401217/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:391678/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:384370/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:373997/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:367326/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:357117/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:347092/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:340727/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:331719/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:328189/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:320890/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:308721/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:292093/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:288203/3‑62 (MQ=255)
cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:278860/3‑62 (MQ=255)
cagCTTTATCCTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt   >  1:987432/3‑62 (MQ=255)
           |                                                    
CGGCTTTATCGGCCGAAGCAGGTAGAGGGGCAGTAAGCAGACGTTGCTTACTTTTGAAAGGCTT  >  minE/2565269‑2565332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑