| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 2,658,121 | (TCA)3→2 | coding (960‑962/1506 nt) | glpD ← | sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,658,115 | 0 | T | . | 100.0% | 20.2 / NA | 6 | coding (968/1506 nt) | glpD | sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (6/0); total (6/0) | |||||||||||
| * | minE | 2,658,116 | 0 | C | . | 100.0% | 20.2 / NA | 6 | coding (967/1506 nt) | glpD | sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base . (6/0); total (6/0) | |||||||||||
| * | minE | 2,658,117 | 0 | A | . | 100.0% | 20.2 / NA | 6 | coding (966/1506 nt) | glpD | sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (6/0); total (6/0) | |||||||||||
GCGAGTCGGACTCATCATCACACAGCGGACGCACACCGGAGTAGGTCCAGACGATATCGTCACGG > minE/2658104‑2658168 ||| gcgAGTCGGAC‑‑‑TCATCACACAGCGGACGCACACCGGAGTAGGTCCa > 1:1239427/1‑46 (MQ=255)gcgAGTCGGAC‑‑‑TCATCACACAGCGGACGCACACCGGAGTAGGTCCAGACGATATCGTCACgg > 1:176001/1‑62 (MQ=255)gcgAGTCGGAC‑‑‑TCATCACACAGCGGACGCACACCGGAGTAGGTCCAGACGATATCGTCACgg > 1:176504/1‑62 (MQ=255)gcgAGTCGGAC‑‑‑TCATCACACAGCGGACGCACACCGGAGTAGGTCCAGACGATATCGTCACgg > 1:240773/1‑62 (MQ=255)gcgAGTCGGAC‑‑‑TCATCACACAGCGGACGCACACCGGAGTAGGTCCAGACGATATCGTCACgg > 1:314775/1‑62 (MQ=255)gcgAGTCGGAC‑‑‑TCATCACACAGCGGACGCACACCGGAGTAGGTCCAGACGATATCGTCACg > 1:135587/1‑61 (MQ=255) ||| GCGAGTCGGACTCATCATCACACAGCGGACGCACACCGGAGTAGGTCCAGACGATATCGTCACGG > minE/2658104‑2658168 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |