Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	22,075	+T	coding (2007/2817 nt)	ileS →	isoleucyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	22,071	1	.	T	100.0%	86.9 / NA	29	G668V (GGT→GTT)	ileS	isoleucyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (0/29);  total (0/29)

ACACCGCGCGCTTCCTGCTGGCAAACCTGAACGG‑TTTTGATCCAGCAAAAGATATGGTGAA  >  minE/22038‑22098                                   |                            acacCGCGCTCTTCCTGCTGGCAAACCTGAACGGTTTTTGATCCAGCAAAAGATATGGTGaa  <  1:669550/62‑1 (MQ=255) acacCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTTGATCCAGCAAAAGATATTGTGaa  <  1:650349/62‑1 (MQ=255) acacCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTTGATCCAGCAAAAGATATGGTGaa  <  1:1675477/62‑1 (MQ=255) acacCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTTGATCCAGCAAAAGATATGGTGaa  <  1:956936/62‑1 (MQ=255) acacCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTTGATCCAGCAAAAGATATGGTGaa  <  1:771072/62‑1 (MQ=255) acacCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTTGATCCAGCAAAAGATATGGTGaa  <  1:762695/62‑1 (MQ=255) acacCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTTGATCCAGCAAAAGATATGGTGaa  <  1:538569/62‑1 (MQ=255) acacCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTTGATCCAGCAAAAGATATGGTGaa  <  1:498539/62‑1 (MQ=255) acacCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTTGATCCAGCAAAAGATATGGTGaa  <  1:446596/62‑1 (MQ=255) acacCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTTGATCCAGCAAAAGATATGGTGaa  <  1:412010/62‑1 (MQ=255) acacCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTTGATCCAGCAAAAGATATGGTGaa  <  1:1847841/62‑1 (MQ=255) acacCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTTGATCCAGCAAAAGATATGGTGaa  <  1:182511/62‑1 (MQ=255) acacCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTTGATCCAGCAAAAGATATGGTGaa  <  1:1725470/62‑1 (MQ=255) acacCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTTGATCCAGCAAAAGATATGGTGaa  <  1:1691451/62‑1 (MQ=255) acacCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTTGATCCAGCAAAAGATATGGTGaa  <  1:1685598/62‑1 (MQ=255) acacCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTTGATCCAGCAAAAGATATGGTGaa  <  1:110251/62‑1 (MQ=255) acacCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTTGATCCAGCAAAAGATATGGTGaa  <  1:1650650/62‑1 (MQ=255) acacCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTTGATCCAGCAAAAGATATGGTGaa  <  1:1613106/62‑1 (MQ=255) acacCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTTGATCCAGCAAAAGATATGGTGaa  <  1:1482933/62‑1 (MQ=255) acacCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTTGATCCAGCAAAAGATATGGTGaa  <  1:1372774/62‑1 (MQ=255) acacCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTTGATCCAGCAAAAGATATGGTGaa  <  1:1323028/62‑1 (MQ=255) acacCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTTGATCCAGCAAAAGATATGGTGaa  <  1:1321593/62‑1 (MQ=255) acacCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTTGATCCAGCAAAAGATATGGTGaa  <  1:118493/62‑1 (MQ=255) acacCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTTGATCCAGCAAAAGATATGGTGaa  <  1:1170933/62‑1 (MQ=255) acacCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTTGATCCAGCAAAAGATATGGTGaa  <  1:1158427/62‑1 (MQ=255) acacCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTTGATCCAGCAAAAGATATGGTGaa  <  1:1155876/62‑1 (MQ=255)  cacCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTTGATCCAGCAAAAGATATGGTGaa  <  1:1637698/61‑1 (MQ=255)  cacCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTTGATCCAGCAAAAGATATGGTGaa  <  1:1545618/61‑1 (MQ=255)                       aaaCCTGAACGGTTTTTGATCCAGCAAAAGATATGGTGaa  <  1:1468319/40‑1 (MQ=38)                                   |                            ACACCGCGCGCTTCCTGCTGGCAAACCTGAACGG‑TTTTGATCCAGCAAAAGATATGGTGAA  >  minE/22038‑22098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑