Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 586,692 G→A L324L (CTG→TTG)  cydD ← fused cysteine transporter subunits and membrane component and ATP‑binding component of ABC superfamily

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE586,6920GA100.0% 405.4 / NA 121L324L (CTG→TTG) cydDfused cysteine transporter subunits and membrane component and ATP‑binding component of ABC superfamily
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/121);  total (0/121)

Only 100 of 121 total aligned reads displayed.

TGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGCCTG  >  minE/586655‑586716
                                     |                        
tGCGGATGTGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:501177/62‑1 (MQ=255)
tGCGGATGGGCGAGCTGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:1865034/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:28487/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:53327/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:532485/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:532280/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:460637/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:442099/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:364050/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:344339/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:307430/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:290590/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:544509/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:278459/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:267432/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:249665/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:101752/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:214159/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:1881623/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:1841702/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:1835836/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:1770379/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:755589/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:967749/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:912706/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:860348/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:848851/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:846624/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:837024/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:827426/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:794901/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:791851/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:755766/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:234978/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:750644/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:73207/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:695292/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:672128/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:656928/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:607582/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:594622/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:585686/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:578241/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:556985/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:118726/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:1462754/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:141787/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:1407433/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:1379400/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:1721898/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:1336424/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:1314313/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:1279142/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:1276910/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:1253246/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:1209143/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:1352025/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:1156401/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:1130022/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:1115460/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:107573/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:1075726/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:1075024/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:1052311/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:1052283/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:1044514/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:1031568/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:1621762/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:1531301/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:1568606/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:1530884/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:1569894/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:1500425/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:1571530/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:1636588/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:1673947/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:168760/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:1472042/62‑1 (MQ=255)
tGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:1688431/62‑1 (MQ=255)
ggCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:167524/61‑1 (MQ=255)
 gCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:745135/61‑1 (MQ=255)
 gCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:83589/61‑1 (MQ=255)
 gCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:920409/61‑1 (MQ=255)
 gCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:957397/61‑1 (MQ=255)
 gCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:1139372/61‑1 (MQ=255)
 gCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:751893/61‑1 (MQ=255)
 gCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:472169/61‑1 (MQ=255)
 gCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:672442/61‑1 (MQ=255)
 gCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:642821/61‑1 (MQ=255)
 gCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:1491253/61‑1 (MQ=255)
 gCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:362820/61‑1 (MQ=255)
 gCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:37008/61‑1 (MQ=255)
 gCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:1359845/61‑1 (MQ=255)
 gCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:1463371/61‑1 (MQ=255)
       gggCGAGCGGGGTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:421923/55‑1 (MQ=255)
               ggggTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:428048/47‑1 (MQ=255)
                gggTTTCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:1480810/46‑1 (MQ=255)
                   tttCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:339203/43‑1 (MQ=255)
                    ttCCATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:1160737/42‑1 (MQ=255)
                      ccATAAACGTTTTCAAACTGTCAGCTGCACCAACAGCCTg  <  1:1121288/40‑1 (MQ=255)
                                     |                        
TGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGCCTG  >  minE/586655‑586716

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑