Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	784,369	A→G	N324D (AAC→GAC)	umuC →	DNA polymerase V, subunit C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	784,369	0	A	G	100.0%	64.8 / NA	18	N324D (AAC→GAC)	umuC	DNA polymerase V, subunit C
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/0);  total (18/0)

ACGGCAATAGCGCGTCGGTAAAACTGCTGACGCCCACTCAGGACAGCAGGGATATCATTAACG  >  minE/784310‑784372                                                            |    aCGGCAATAGCGCGTCGGTAAAACTGCTGACGCCCACTCAGGACAGCAGGGATATCATTGAc   >  1:1596912/1‑62 (MQ=255) aCGGCAATAGCGCGTCGGTAAAACTGCTGACGCCCACTCAGGACAGCAGGGATATCATTGAc   >  1:478687/1‑62 (MQ=255) aCGGCAATAGCGCGTCGGTAAAACTGCTGACGCCCACTCAGGACAGCAGGGATATCATTGAc   >  1:29472/1‑62 (MQ=255) aCGGCAATAGCGCGTCGGTAAAACTGCTGACGCCCACTCAGGACAGCAGGGATATCATTGAc   >  1:28204/1‑62 (MQ=255) aCGGCAATAGCGCGTCGGTAAAACTGCTGACGCCCACTCAGGACAGCAGGGATATCATTGAc   >  1:210832/1‑62 (MQ=255) aCGGCAATAGCGCGTCGGTAAAACTGCTGACGCCCACTCAGGACAGCAGGGATATCATTGAc   >  1:1861010/1‑62 (MQ=255) aCGGCAATAGCGCGTCGGTAAAACTGCTGACGCCCACTCAGGACAGCAGGGATATCATTGAc   >  1:1782713/1‑62 (MQ=255) aCGGCAATAGCGCGTCGGTAAAACTGCTGACGCCCACTCAGGACAGCAGGGATATCATTGAc   >  1:1771060/1‑62 (MQ=255) aCGGCAATAGCGCGTCGGTAAAACTGCTGACGCCCACTCAGGACAGCAGGGATATCATTGAc   >  1:1068818/1‑62 (MQ=255) aCGGCAATAGCGCGTCGGTAAAACTGCTGACGCCCACTCAGGACAGCAGGGATATCATTGAc   >  1:1595207/1‑62 (MQ=255) aCGGCAATAGCGCGTCGGTAAAACTGCTGACGCCCACTCAGGACAGCAGGGATATCATTGAc   >  1:1567624/1‑62 (MQ=255) aCGGCAATAGCGCGTCGGTAAAACTGCTGACGCCCACTCAGGACAGCAGGGATATCATTGAc   >  1:1547476/1‑62 (MQ=255) aCGGCAATAGCGCGTCGGTAAAACTGCTGACGCCCACTCAGGACAGCAGGGATATCATTGAc   >  1:1537661/1‑62 (MQ=255) aCGGCAATAGCGCGTCGGTAAAACTGCTGACGCCCACTCAGGACAGCAGGGATATCATTGAc   >  1:1333696/1‑62 (MQ=255) aCGGCAATAGCGCGTCGGTAAAACTGCTGACGCCCACTCAGGACAGCAGGGATATCATTGAc   >  1:1329044/1‑62 (MQ=255) aCGGCAATAGCGCGTCGGTAAAACTGCTGACGCCCACTCAGGACAGCAGGGATATCATTGAc   >  1:1290141/1‑62 (MQ=255) aCGGCAATAGCGCGTCGGTAAAACTGCTGACGCCCACTCAGGACAGCAGGGATATCATTGAc   >  1:1284304/1‑62 (MQ=255)  cGGCAATAGCGCGTCGGTAAAACTGCTGACGCCCACTCAGGACAGCAGGGATATCATTGACg  >  1:1895999/1‑62 (MQ=255)                                                            |    ACGGCAATAGCGCGTCGGTAAAACTGCTGACGCCCACTCAGGACAGCAGGGATATCATTAACG  >  minE/784310‑784372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑