Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	821,218	G→A	L280L (CTG→CTA)	kdsA →	3‑deoxy‑D‑manno‑octulosonate 8‑phosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	821,218	0	G	A	100.0%	86.2 / NA	27	L280L (CTG→CTA)	kdsA	3‑deoxy‑D‑manno‑octulosonate 8‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/27);  total (0/27)

GGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTT  >  minE/821204‑821266               |                                                 ggTTTCGAAGAACTAGATACCAGCAAGTAAT‑ATTTTTGCTTGAAAAATAAAGTATTAGCGtt  <  1:1506097/62‑1 (MQ=255)  gTTTCGAAGAACTAGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGtt  <  1:1701098/62‑1 (MQ=255)  gTTTCGAAGAACTAGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGtt  <  1:985793/62‑1 (MQ=255)  gTTTCGAAGAACTAGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGtt  <  1:886472/62‑1 (MQ=255)  gTTTCGAAGAACTAGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGtt  <  1:741370/62‑1 (MQ=255)  gTTTCGAAGAACTAGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGtt  <  1:699976/62‑1 (MQ=255)  gTTTCGAAGAACTAGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGtt  <  1:4490/62‑1 (MQ=255)  gTTTCGAAGAACTAGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGtt  <  1:422736/62‑1 (MQ=255)  gTTTCGAAGAACTAGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGtt  <  1:390233/62‑1 (MQ=255)  gTTTCGAAGAACTAGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGtt  <  1:279745/62‑1 (MQ=255)  gTTTCGAAGAACTAGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGtt  <  1:1887372/62‑1 (MQ=255)  gTTTCGAAGAACTAGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGtt  <  1:1867884/62‑1 (MQ=255)  gTTTCGAAGAACTAGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGtt  <  1:1766522/62‑1 (MQ=255)  gTTTCGAAGAACTAGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGtt  <  1:1728831/62‑1 (MQ=255)  gTTTCGAAGAACTAGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGtt  <  1:1146184/62‑1 (MQ=255)  gTTTCGAAGAACTAGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGtt  <  1:1661021/62‑1 (MQ=255)  gTTTCGAAGAACTAGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGtt  <  1:1657192/62‑1 (MQ=255)  gTTTCGAAGAACTAGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGtt  <  1:1627822/62‑1 (MQ=255)  gTTTCGAAGAACTAGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGtt  <  1:1560802/62‑1 (MQ=255)  gTTTCGAAGAACTAGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGtt  <  1:1552596/62‑1 (MQ=255)  gTTTCGAAGAACTAGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGtt  <  1:1540180/62‑1 (MQ=255)  gTTTCGAAGAACTAGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGtt  <  1:1394411/62‑1 (MQ=255)  gTTTCGAAGAACTAGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGtt  <  1:1301736/62‑1 (MQ=255)  gTTTCGAAGAACTAGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGtt  <  1:125701/62‑1 (MQ=255)  gTTTCGAAGAACTAGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGtt  <  1:1214095/62‑1 (MQ=255)  gTTTCGAAGAACTAGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGtt  <  1:1213482/62‑1 (MQ=255)   tttCGAAGAACTAGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGtt  <  1:44180/61‑1 (MQ=255)               |                                                 GGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTT  >  minE/821204‑821266

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑