Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 872,997 G→A G63G (GGG→GGA)  cysB → DNA‑binding transcriptional dual regulator, O‑acetyl‑L‑serine‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE872,9970GA100.0% 728.9 / NA 215G63G (GGG→GGA) cysBDNA‑binding transcriptional dual regulator, O‑acetyl‑L‑serine‑binding
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/215);  total (0/215)

Only 100 of 215 total aligned reads displayed.

TCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCG  >  minE/872956‑873016
                                         |                   
tCCCTAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:1077788/61‑1 (MQ=255)
tCCCGATGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:1823842/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGTAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:666227/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGATAAGAAATAATTCGTAtcg  <  1:1485496/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAACTAATTCGTAtcg  <  1:64924/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:276297/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:530182/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:513805/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:499409/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:486429/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:403581/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:355215/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:333199/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:323769/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:315232/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:302734/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:560881/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:265223/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:257353/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:251857/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:240730/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:233089/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:227957/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:209188/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:196483/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:194669/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:1886520/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:77114/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:96049/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:935872/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:88952/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:870241/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:850950/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:82667/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:821887/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:818639/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:804910/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:800850/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:782105/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:549238/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:767977/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:73194/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:716101/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:710027/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:697241/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:690167/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:635287/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:585805/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:567213/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:1877936/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:1166207/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:137880/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:1347864/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:1312457/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:1292090/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:1268614/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:1257201/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:1248195/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:1235639/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:1218843/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:1186662/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:1333932/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:1148240/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:1116499/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:1106824/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:1095246/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:1073193/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:1065559/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:1052031/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:1028947/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:1015540/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:1634732/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:1856688/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:1809682/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:1704180/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:1689802/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:1686267/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:1675630/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:1668426/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:1641862/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:1591174/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:1586706/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:1537934/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:1425062/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:1447319/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:1490673/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:1500625/61‑1 (MQ=255)
tCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:1555830/61‑1 (MQ=255)
tCCCGAAGCGGAAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:1663987/61‑1 (MQ=255)
tCCCGAAGCGGAAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:607111/61‑1 (MQ=255)
 cccGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:1282888/60‑1 (MQ=255)
 cccGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:78589/60‑1 (MQ=255)
 cccGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:1169729/60‑1 (MQ=255)
 cccGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:1013698/60‑1 (MQ=255)
 cccGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:1525125/60‑1 (MQ=255)
 cccGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:1751245/60‑1 (MQ=255)
    gAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:1410177/57‑1 (MQ=255)
        cGGCAAGCACCTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:1159348/53‑1 (MQ=255)
                 ccTGACGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:1396041/44‑1 (MQ=255)
                     aCGCAGGTAACGCCAGCAGGACAAGAAATAATTCGTAtcg  <  1:929938/40‑1 (MQ=255)
                                         |                   
TCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCG  >  minE/872956‑873016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑