Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,211,696	G→A	A461V (GCG→GTG)	rng ←	ribonuclease G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,211,696	0	G	A	100.0%	123.1 / NA	38	A461V (GCG→GTG)	rng	ribonuclease G
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/25);  total (13/25)

TTGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCT  >  minE/2211656‑2211734                                         |                                       ttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCACCAGCGAGTGTGTCTCTTCGc                   <  1:36354/62‑1 (MQ=255) ttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGc                   <  1:1085958/62‑1 (MQ=255) ttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGc                   <  1:994489/62‑1 (MQ=255) ttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGc                   <  1:989336/62‑1 (MQ=255) ttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGc                   <  1:952803/62‑1 (MQ=255) ttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGc                   <  1:793964/62‑1 (MQ=255) ttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGc                   <  1:705402/62‑1 (MQ=255) ttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGc                   <  1:464437/62‑1 (MQ=255) ttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGc                   <  1:378066/62‑1 (MQ=255) ttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGc                   <  1:289506/62‑1 (MQ=255) ttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGc                   <  1:213882/62‑1 (MQ=255) ttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGc                   <  1:1899303/62‑1 (MQ=255) ttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGc                   <  1:1844423/62‑1 (MQ=255) ttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGc                   <  1:1736568/62‑1 (MQ=255) ttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGc                   <  1:1552586/62‑1 (MQ=255) ttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGc                   <  1:1182083/62‑1 (MQ=255) ttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGc                   <  1:1428165/62‑1 (MQ=255) ttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGc                   <  1:1040738/62‑1 (MQ=255) ttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGc                   <  1:1297715/62‑1 (MQ=255) ttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGc                   <  1:1104901/62‑1 (MQ=255) ttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGc                   <  1:1188067/62‑1 (MQ=255) ttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGc                   <  1:1637190/62‑1 (MQ=255) ttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGc                   <  1:1272769/62‑1 (MQ=255)  tGTACTTTAACCTGTTTGCCAACTAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGc                   <  1:83745/61‑1 (MQ=255)  tGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGc                   <  1:1285647/61‑1 (MQ=255)                         gAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGCCTTTCACGGCTTCAGCt  >  1:974184/1‑55 (MQ=255)                         gAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCa     >  1:1842887/1‑52 (MQ=255)                         gAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCt  >  1:1126522/1‑55 (MQ=255)                         gAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCt  >  1:474128/1‑55 (MQ=255)                         gAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCt  >  1:939420/1‑55 (MQ=255)                         gAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCt  >  1:994832/1‑55 (MQ=255)                         gAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCt  >  1:889282/1‑55 (MQ=255)                         gAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCt  >  1:753015/1‑55 (MQ=255)                         gAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCt  >  1:1248157/1‑55 (MQ=255)                         gAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCt  >  1:1279584/1‑55 (MQ=255)                         gAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCt  >  1:253222/1‑55 (MQ=255)                         gAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCt  >  1:1410096/1‑55 (MQ=255)                         gAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCt  >  1:1722299/1‑55 (MQ=255)                                         |                                       TTGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCT  >  minE/2211656‑2211734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑