Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,335,701	A→G	E29E (GAA→GAG)	fdoH →	formate dehydrogenase‑O, Fe‑S subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,335,701	0	A	G	100.0%	98.6 / NA	27	E29E (GAA→GAG)	fdoH	formate dehydrogenase‑O, Fe‑S subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/0);  total (27/0)

CTAACGGTCTGACCCCCGCGCCTCAGGCGCGGGACTTCCAGGAAGAAGTGGCGAAACTCATC  >  minE/2335655‑2335716                                               |                cTAACGGTCTGACCCCCGCGCCTCAGGCGCGGGACTTCCAGGAAGAGGTGGCGAAAc       >  1:1012612/1‑57 (MQ=255) cTAACGGTCTGACCCCCGCGCCTCAGGCGCGGGACTTCCAGGAAGAGGTGGCGAAACTCATc  >  1:1730402/1‑62 (MQ=255) cTAACGGTCTGACCCCCGCGCCTCAGGCGCGGGACTTCCAGGAAGAGGTGGCGAAACTCATc  >  1:954112/1‑62 (MQ=255) cTAACGGTCTGACCCCCGCGCCTCAGGCGCGGGACTTCCAGGAAGAGGTGGCGAAACTCATc  >  1:934232/1‑62 (MQ=255) cTAACGGTCTGACCCCCGCGCCTCAGGCGCGGGACTTCCAGGAAGAGGTGGCGAAACTCATc  >  1:923747/1‑62 (MQ=255) cTAACGGTCTGACCCCCGCGCCTCAGGCGCGGGACTTCCAGGAAGAGGTGGCGAAACTCATc  >  1:907605/1‑62 (MQ=255) cTAACGGTCTGACCCCCGCGCCTCAGGCGCGGGACTTCCAGGAAGAGGTGGCGAAACTCATc  >  1:898240/1‑62 (MQ=255) cTAACGGTCTGACCCCCGCGCCTCAGGCGCGGGACTTCCAGGAAGAGGTGGCGAAACTCATc  >  1:830481/1‑62 (MQ=255) cTAACGGTCTGACCCCCGCGCCTCAGGCGCGGGACTTCCAGGAAGAGGTGGCGAAACTCATc  >  1:776052/1‑62 (MQ=255) cTAACGGTCTGACCCCCGCGCCTCAGGCGCGGGACTTCCAGGAAGAGGTGGCGAAACTCATc  >  1:309141/1‑62 (MQ=255) cTAACGGTCTGACCCCCGCGCCTCAGGCGCGGGACTTCCAGGAAGAGGTGGCGAAACTCATc  >  1:264233/1‑62 (MQ=255) cTAACGGTCTGACCCCCGCGCCTCAGGCGCGGGACTTCCAGGAAGAGGTGGCGAAACTCATc  >  1:1763381/1‑62 (MQ=255) cTAACGGTCTGACCCCCGCGCCTCAGGCGCGGGACTTCCAGGAAGAGGTGGCGAAACTCATc  >  1:1758337/1‑62 (MQ=255) cTAACGGTCTGACCCCCGCGCCTCAGGCGCGGGACTTCCAGGAAGAGGTGGCGAAACTCATc  >  1:1731024/1‑62 (MQ=255) cTAACGGTCTGACCCCCGCGCCTCAGGCGCGGGACTTCCAGGAAGAGGTGGCGAAACTCATc  >  1:100344/1‑62 (MQ=255) cTAACGGTCTGACCCCCGCGCCTCAGGCGCGGGACTTCCAGGAAGAGGTGGCGAAACTCATc  >  1:1632169/1‑62 (MQ=255) cTAACGGTCTGACCCCCGCGCCTCAGGCGCGGGACTTCCAGGAAGAGGTGGCGAAACTCATc  >  1:1427694/1‑62 (MQ=255) cTAACGGTCTGACCCCCGCGCCTCAGGCGCGGGACTTCCAGGAAGAGGTGGCGAAACTCATc  >  1:142367/1‑62 (MQ=255) cTAACGGTCTGACCCCCGCGCCTCAGGCGCGGGACTTCCAGGAAGAGGTGGCGAAACTCATc  >  1:1364282/1‑62 (MQ=255) cTAACGGTCTGACCCCCGCGCCTCAGGCGCGGGACTTCCAGGAAGAGGTGGCGAAACTCATc  >  1:1363564/1‑62 (MQ=255) cTAACGGTCTGACCCCCGCGCCTCAGGCGCGGGACTTCCAGGAAGAGGTGGCGAAACTCATc  >  1:126480/1‑62 (MQ=255) cTAACGGTCTGACCCCCGCGCCTCAGGCGCGGGACTTCCAGGAAGAGGTGGCGAAACTCATc  >  1:1159724/1‑62 (MQ=255) cTAACGGTCTGACCCCCGCGCCTCAGGCGCGGGACTTCCAGGAAGAGGTGGCGAAACTCATc  >  1:1113644/1‑62 (MQ=255) cTAACGGTCTGACCCCCGCGCCTCAGGCGCGGGACTTCCAGGAAGAGGTGGCGAAACTCATc  >  1:109613/1‑62 (MQ=255) cTAACGGTCTGACCCCCGCGCCTCAGGCGCGGGACTTCCAGGAAGAGGTGGCGAAACTCATc  >  1:1051389/1‑62 (MQ=255) cTAACGGTCTGACCCCCGCGCCTCAGGCGCGGGACTTCCAGGAAGAGGTGGCGAAACTCATc  >  1:1011887/1‑62 (MQ=255) cTAACGGTCTGACCCCCGCGCCTCAGGAGCGGGACTTCCAGGAAGAGGTGGCGAAACTCATc  >  1:340976/1‑62 (MQ=39)                                               |                CTAACGGTCTGACCCCCGCGCCTCAGGCGCGGGACTTCCAGGAAGAAGTGGCGAAACTCATC  >  minE/2335655‑2335716

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑