Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,427,554	A→G	D309D (GAT→GAC)	rffE ←	UDP‑N‑acetyl glucosamine‑2‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,427,554	0	A	G	100.0%	33.7 / NA	10	D309D (GAT→GAC)	rffE	UDP‑N‑acetyl glucosamine‑2‑epimerase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/10);  total (0/10)

GACGCTCAGTGGTATCGCGCATCACCAGCACAGGTTTCCCCAGCGAAGGCGC  >  minE/2427541‑2427592              |                                       gACGTTCGGTGGTGTCGCGCATCACCAGGACCGGCTTGCCGAGCGATGgcgc  <  1:1332451/52‑1 (MQ=255) gACGTTCGGTGGTGTCGCGCATCACCAGGACCGGCTTGCCGAGCGATGgcgc  <  1:236259/52‑1 (MQ=255) gACGTTCGGTGGTGTCGCGCATCACCAGGACCGGCTTGCCGAGCGATGgcgc  <  1:240261/52‑1 (MQ=255) gACGTTCGGTGGTGTCGCGCATCACCAGGACCGGCTTGCCGAGCGATGgcgc  <  1:279156/52‑1 (MQ=255) gACGTTCGGTGGTGTCGCGCATCACCAGGACCGGCTTGCCGAGCGATGgcgc  <  1:292840/52‑1 (MQ=255) gACGTTCGGTGGTGTCGCGCATCACCAGGACCGGCTTGCCGAGCGATGgcgc  <  1:317166/52‑1 (MQ=255) gACGTTCGGTGGTGTCGCGCATCACCAGGACCGGCTTGCCGAGCGATGgcgc  <  1:619459/52‑1 (MQ=255) gACGTTCGGTGGTGTCGCGCATCACCAGGACCGGCTTGCCGAGCGATGgcgc  <  1:775737/52‑1 (MQ=255) gACGTTCGGTGGTGTCGCGCATCACCAGGACCGGCTTGCCGAGCGATGgcgc  <  1:786082/52‑1 (MQ=255) gACGTTCGGTGGTGTCGCGCATCACCAGGACCGGCTTGCCGAGCGATGgcgc  <  1:916499/52‑1 (MQ=255)              |                                       GACGCTCAGTGGTATCGCGCATCACCAGCACAGGTTTCCCCAGCGAAGGCGC  >  minE/2427541‑2427592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑