Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,560,276	C→T	intergenic (‑16/+182)	yibK ← / ← lldD	predicted rRNA methylase/L‑lactate dehydrogenase, FMN‑linked

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,560,276	0	C	T	100.0%	166.7 / NA	51	intergenic (‑16/+182)	yibK/lldD	predicted rRNA methylase/L‑lactate dehydrogenase, FMN‑linked
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (51/0);  total (51/0)

GGCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAG  >  minE/2560223‑2560284                                                      |         ggCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGTGGGGGCa   >  1:1851577/1‑61 (MQ=255) ggCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGTGGGGGCa   >  1:292485/1‑61 (MQ=255) ggCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGTGGGGGCa   >  1:1318155/1‑61 (MQ=255) ggCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGTGGGGGCa   >  1:1504347/1‑61 (MQ=255) ggCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGTGGGGGCa   >  1:682517/1‑61 (MQ=255) ggCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGTGGGGGCAg  >  1:586750/1‑62 (MQ=255) ggCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGTGGGGGCAg  >  1:242985/1‑62 (MQ=255) ggCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGTGGGGGCAg  >  1:329970/1‑62 (MQ=255) ggCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGTGGGGGCAg  >  1:395526/1‑62 (MQ=255) ggCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGTGGGGGCAg  >  1:403378/1‑62 (MQ=255) ggCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGTGGGGGCAg  >  1:434445/1‑62 (MQ=255) ggCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGTGGGGGCAg  >  1:444069/1‑62 (MQ=255) ggCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGTGGGGGCAg  >  1:513804/1‑62 (MQ=255) ggCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGTGGGGGCAg  >  1:520978/1‑62 (MQ=255) ggCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGTGGGGGCAg  >  1:552505/1‑62 (MQ=255) ggCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGTGGGGGCAg  >  1:586016/1‑62 (MQ=255) ggCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGTGGGGGCAg  >  1:1780889/1‑62 (MQ=255) ggCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGTGGGGGCAg  >  1:7280/1‑62 (MQ=255) ggCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGTGGGGGCAg  >  1:730474/1‑62 (MQ=255) ggCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGTGGGGGCAg  >  1:736994/1‑62 (MQ=255) ggCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGTGGGGGCAg  >  1:780257/1‑62 (MQ=255) ggCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGTGGGGGCAg  >  1:816285/1‑62 (MQ=255) ggCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGTGGGGGCAg  >  1:833566/1‑62 (MQ=255) ggCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGTGGGGGCAg  >  1:873540/1‑62 (MQ=255) ggCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGTGGGGGCAg  >  1:891318/1‑62 (MQ=255) ggCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGTGGGGGCAg  >  1:980577/1‑62 (MQ=255) ggCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGTGGGGGCAg  >  1:986162/1‑62 (MQ=255) ggCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGTGGGGGCAg  >  1:1433494/1‑62 (MQ=255) ggCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGTGGGGGCAg  >  1:1059972/1‑62 (MQ=255) ggCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGTGGGGGCAg  >  1:1159130/1‑62 (MQ=255) ggCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGTGGGGGCAg  >  1:1186293/1‑62 (MQ=255) ggCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGTGGGGGCAg  >  1:1221390/1‑62 (MQ=255) ggCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGTGGGGGCAg  >  1:1260000/1‑62 (MQ=255) ggCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGTGGGGGCAg  >  1:1295248/1‑62 (MQ=255) ggCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGTGGGGGCAg  >  1:1351165/1‑62 (MQ=255) ggCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGTGGGGGCAg  >  1:1352286/1‑62 (MQ=255) ggCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGTGGGGGCAg  >  1:1372864/1‑62 (MQ=255) ggCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGTGGGGGCAg  >  1:139677/1‑62 (MQ=255) ggCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGTGGGGGCAg  >  1:1425779/1‑62 (MQ=255) ggCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGTGGGGGCAg  >  1:1819985/1‑62 (MQ=255) ggCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGTGGGGGCAg  >  1:1445298/1‑62 (MQ=255) ggCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGTGGGGGCAg  >  1:1446043/1‑62 (MQ=255) ggCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGTGGGGGCAg  >  1:1501299/1‑62 (MQ=255) ggCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGTGGGGGCAg  >  1:1630804/1‑62 (MQ=255) ggCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGTGGGGGCAg  >  1:1647606/1‑62 (MQ=255) ggCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGTGGGGGCAg  >  1:1672668/1‑62 (MQ=255) ggCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGTGGGGGCAg  >  1:173035/1‑62 (MQ=255) ggCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGTGGGGGCAg  >  1:1737643/1‑62 (MQ=255) ggCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGTGGGGGCAg  >  1:175284/1‑62 (MQ=255) ggCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGTGGGGGCAg  >  1:1771361/1‑62 (MQ=255) ggCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGTGGGGGCAg  >  1:1019471/1‑62 (MQ=255)                                                      |         GGCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAG  >  minE/2560223‑2560284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑