Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,730,912	G→T	intergenic (‑181/‑40)	slyX ← / → fkpA	conserved hypothetical protein/FKBP‑type peptidyl‑prolyl cis‑trans isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,730,912	0	G	T	96.9%	94.1 / ‑2.7	32	intergenic (‑181/‑40)	slyX/fkpA	conserved hypothetical protein/FKBP‑type peptidyl‑prolyl cis‑trans isomerase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (31/0);  total (32/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

ACAACGCTTTATAGTACCCTTCTGATAATAGTTAACCCTGGGGTGAGATGCCCCGATCC  >  minE/2730882‑2730940                               |                             aCAACGCTTTATAGTACCCTTCTGTTAATATTTAACCCTGGGGTGAGATGCCCCGATcc  >  1:119494/1‑59 (MQ=255) aCAACGCTTTATAGTACCCTTCTGATAATATTTAACCCTGGGGTGAGATGCCCCGATcc  >  1:1801971/1‑59 (MQ=255) aCAACGCTTTATAGTACCCTTCTGATAATATTTAACCCTGGGGTGAGATGCCCCGATcc  >  1:991269/1‑59 (MQ=255) aCAACGCTTTATAGTACCCTTCTGATAATATTTAACCCTGGGGTGAGATGCCCCGATcc  >  1:91659/1‑59 (MQ=255) aCAACGCTTTATAGTACCCTTCTGATAATATTTAACCCTGGGGTGAGATGCCCCGATcc  >  1:87615/1‑59 (MQ=255) aCAACGCTTTATAGTACCCTTCTGATAATATTTAACCCTGGGGTGAGATGCCCCGATcc  >  1:771290/1‑59 (MQ=255) aCAACGCTTTATAGTACCCTTCTGATAATATTTAACCCTGGGGTGAGATGCCCCGATcc  >  1:699854/1‑59 (MQ=255) aCAACGCTTTATAGTACCCTTCTGATAATATTTAACCCTGGGGTGAGATGCCCCGATcc  >  1:63743/1‑59 (MQ=255) aCAACGCTTTATAGTACCCTTCTGATAATATTTAACCCTGGGGTGAGATGCCCCGATcc  >  1:633198/1‑59 (MQ=255) aCAACGCTTTATAGTACCCTTCTGATAATATTTAACCCTGGGGTGAGATGCCCCGATcc  >  1:546477/1‑59 (MQ=255) aCAACGCTTTATAGTACCCTTCTGATAATATTTAACCCTGGGGTGAGATGCCCCGATcc  >  1:516764/1‑59 (MQ=255) aCAACGCTTTATAGTACCCTTCTGATAATATTTAACCCTGGGGTGAGATGCCCCGATcc  >  1:496395/1‑59 (MQ=255) aCAACGCTTTATAGTACCCTTCTGATAATATTTAACCCTGGGGTGAGATGCCCCGATcc  >  1:453853/1‑59 (MQ=255) aCAACGCTTTATAGTACCCTTCTGATAATATTTAACCCTGGGGTGAGATGCCCCGATcc  >  1:25842/1‑59 (MQ=255) aCAACGCTTTATAGTACCCTTCTGATAATATTTAACCCTGGGGTGAGATGCCCCGATcc  >  1:215267/1‑59 (MQ=255) aCAACGCTTTATAGTACCCTTCTGATAATATTTAACCCTGGGGTGAGATGCCCCGATcc  >  1:21268/1‑59 (MQ=255) aCAACGCTTTATAGTACCCTTCTGATAATATTTAACCCTGGGGTGAGATGCCCCGATcc  >  1:1787441/1‑59 (MQ=255) aCAACGCTTTATAGTACCCTTCTGATAATATTTAACCCTGGGGTGAGATGCCCCGATcc  >  1:1782281/1‑59 (MQ=255) aCAACGCTTTATAGTACCCTTCTGATAATATTTAACCCTGGGGTGAGATGCCCCGATcc  >  1:1775809/1‑59 (MQ=255) aCAACGCTTTATAGTACCCTTCTGATAATATTTAACCCTGGGGTGAGATGCCCCGATcc  >  1:1676571/1‑59 (MQ=255) aCAACGCTTTATAGTACCCTTCTGATAATATTTAACCCTGGGGTGAGATGCCCCGATcc  >  1:1612503/1‑59 (MQ=255) aCAACGCTTTATAGTACCCTTCTGATAATATTTAACCCTGGGGTGAGATGCCCCGATcc  >  1:1607856/1‑59 (MQ=255) aCAACGCTTTATAGTACCCTTCTGATAATATTTAACCCTGGGGTGAGATGCCCCGATcc  >  1:1599500/1‑59 (MQ=255) aCAACGCTTTATAGTACCCTTCTGATAATATTTAACCCTGGGGTGAGATGCCCCGATcc  >  1:156701/1‑59 (MQ=255) aCAACGCTTTATAGTACCCTTCTGATAATATTTAACCCTGGGGTGAGATGCCCCGATcc  >  1:1469492/1‑59 (MQ=255) aCAACGCTTTATAGTACCCTTCTGATAATATTTAACCCTGGGGTGAGATGCCCCGATcc  >  1:1290278/1‑59 (MQ=255) aCAACGCTTTATAGTACCCTTCTGATAATATTTAACCCTGGGGTGAGATGCCCCGATcc  >  1:1258890/1‑59 (MQ=255) aCAACGCTTTATAGTACCCTTCTGATAATATTTAACCCTGGGGTGAGATGCCCCGATcc  >  1:1245571/1‑59 (MQ=255) aCAACGCTTTATAGTACCCTTCTGATAATATTTAACCCTGGGGTGAGATGCCCCGATcc  >  1:1101786/1‑59 (MQ=255) aCAACGCTTTATAGTACCCTTCTGATAATATTTAACCCTGGGGTGAGATGCCCCGATcc  >  1:1053377/1‑59 (MQ=255) aCAACGCTTTATAGTACCCTTCTGATAATATTTAACCCTGGGGTGAGATGCCACGATcc  >  1:1010889/1‑59 (MQ=255) aCAACGCTTTATAGTACCCTTCTGATAATAGTTAACCCTGGGGTGAGATGCCCCGATcc  >  1:1894646/1‑59 (MQ=255)                               |                             ACAACGCTTTATAGTACCCTTCTGATAATAGTTAACCCTGGGGTGAGATGCCCCGATCC  >  minE/2730882‑2730940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑