Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 473,506 G→A K356K (AAG→AAA)  mngA → fused 2‑O‑a‑mannosyl‑D‑glycerate specific PTS enzyme IIABC components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE473,5060GA100.0% 337.3 / NA 102K356K (AAG→AAA) mngAfused 2‑O‑a‑mannosyl‑D‑glycerate specific PTS enzyme IIABC components
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (102/0);  total (102/0)

Only 100 of 102 total aligned reads displayed.

TTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAGCTGGG  >  minE/473450‑473511
                                                        |     
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggt  >  1:915946/1‑61 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:368187/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:538044/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:518079/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:516260/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:501178/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:487664/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:475890/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:464469/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:460947/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:409988/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:394856/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:390753/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:385755/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:55992/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:332062/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:258742/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:254675/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:253512/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:244348/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:243014/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:200967/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:1884928/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:1027485/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:1866720/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:721969/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:971596/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:944552/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:924671/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:923510/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:865091/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:852955/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:842824/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:797552/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:770984/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:758768/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:73204/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:1879662/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:688956/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:686142/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:673030/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:651230/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:63575/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:628955/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:622075/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:61349/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:604769/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:603264/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:1286912/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:1524033/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:1522422/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:1486813/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:1459431/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:1425512/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:1405080/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:138608/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:1358633/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:1356120/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:1338214/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:1307294/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:1838922/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:1190506/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:1187986/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:118393/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:1169421/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:113174/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:1129495/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:1108309/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:1094679/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:1069626/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:1062682/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:1033304/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:1690537/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:1859478/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:1830654/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:1802574/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:1800338/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:1798351/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:1742470/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:1711589/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:1695136/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:1525201/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:1684783/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:1680525/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:1678978/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:1658915/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:1652397/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:1634666/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:158106/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:1573862/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:1544619/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:1534128/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:1526136/1‑62 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTgg   >  1:1360865/1‑61 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTgg   >  1:1250947/1‑61 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTgg   >  1:1793324/1‑61 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTgg   >  1:192206/1‑61 (MQ=255)
ttCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTgg   >  1:551720/1‑61 (MQ=255)
ttCGGGCTACAAGATCTGATTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAACTggg  >  1:258937/1‑62 (MQ=255)
ttCGGGCAACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGAGGATGTACCGCAAACTggg  >  1:769272/1‑62 (MQ=38)
                                                        |     
TTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAGCTGGG  >  minE/473450‑473511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑