| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 2,952,505 | (G)8→9 | noncoding (72/87 nt) | leuP ← | tRNA‑Leu |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,952,497 | 1 | . | G | 81.2% | 36.0 / 4.9 | 16 | noncoding (80/87 nt) | leuP | tRNA‑Leu |
| Reads supporting (aligned to +/- strand): ref base . (1/2); new base G (6/7); total (7/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01 | |||||||||||
GCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑A‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACC > minE/2952428‑2952559 | gcgcGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑A‑g < 1:3349012/71‑1 (MQ=255)gcgcGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑A‑g < 1:1726933/71‑1 (MQ=255)gcgcGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑A‑g < 1:2972384/71‑1 (MQ=255) cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑A‑g < 1:3918885/70‑1 (MQ=255) tACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑a > 1:94068/1‑63 (MQ=255) tACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑a > 1:1773986/1‑63 (MQ=255) tACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑a > 1:4238408/1‑63 (MQ=255) tACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑a > 1:2180775/1‑63 (MQ=255) ccAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑A‑ggggggg < 1:1230459/68‑1 (MQ=255) ccAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑A‑ggggggg < 1:247419/68‑1 (MQ=255) tCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑A‑g > 1:879678/1‑57 (MQ=255) tCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑A‑g > 1:392835/1‑57 (MQ=255) tCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑A‑g > 1:3963061/1‑57 (MQ=255) tCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑A‑g > 1:3026063/1‑57 (MQ=255) tCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑A‑g > 1:2716454/1‑57 (MQ=255) tCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑A‑g > 1:72576/1‑57 (MQ=255) tCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑A‑g > 1:2056311/1‑57 (MQ=255) tCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑A‑g > 1:1939735/1‑57 (MQ=255) tCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑A‑g > 1:883631/1‑57 (MQ=255) tCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑A‑g > 1:1029015/1‑57 (MQ=255) ccGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGGACTTGaa > 1:1362171/1‑71 (MQ=255) ttCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGGACTTGAACCCCCAc < 1:2395265/70‑1 (MQ=255) aTCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGGACTTGAACCCCCAc < 1:3559298/59‑1 (MQ=37) aTCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGGACTTGAACCCCCACGTCCGTAAg > 1:1093642/1‑68 (MQ=255) aTCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGGACTTGAACCCCCACGTCCGTAAGaa > 1:3613259/1‑70 (MQ=255) aTCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGGACTTGAACCCCCACGTCCGTAAGaa > 1:271549/1‑70 (MQ=255) aTCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGGACTTGAACCCCCACGTCCGTAAGa > 1:3094204/1‑69 (MQ=255) aTCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑A‑gggggggg > 1:1185032/1‑44 (MQ=255) cTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGGACTTGAACCCCCAc < 1:3729535/57‑1 (MQ=25) ttACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGGACTTGAACCCCCAc < 1:4448720/56‑1 (MQ=25) gCCTCGTTTGGTGCG‑AGGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgcg > 1:111233/1‑71 (MQ=255) gCCGCGTTTGGTGCGTG‑GGGGGGGGACTTGAACCCCCACGTCCTTAAGaa < 1:628071/50‑1 (MQ=14) gTTTGGTGCG‑AGGGGGGGGGACTTGAACCCCCACGTCCGTAAg < 1:35436/43‑1 (MQ=11) gTTTGGTGCG‑AGGGGGGGGGACTTGAACCCCCACGTCCGTAAGaa < 1:426931/45‑1 (MQ=11) gTTTGGTGCG‑AGGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAac < 1:2410704/52‑1 (MQ=14) ttGGTGCGTG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAac < 1:3062683/50‑1 (MQ=11) tGGTGCG‑A‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAcc > 1:4322517/1‑70 (MQ=35) | GCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑A‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACC > minE/2952428‑2952559 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |