| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,467,267 | 0 | C | T | 36.0% | 49.0 / 49.1 | 50 | A244V (GCA→GTA) | atpA | F1 sector of membrane‑bound ATP synthase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base C (8/24); new base T (0/18); total (8/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.87e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTGATCATTTACGATGACCTGTC > minE/2467197‑2467327 | acCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTgcgc < 1:2134523/71‑1 (MQ=255) tACCTGGCACCGTATGCCGGTTGCGTAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTg < 1:3226003/66‑1 (MQ=255) tACCTGGCACCGTATGCCGGTTGCGTAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTg < 1:989111/66‑1 (MQ=255) tACCTGGCACCGTATGCCGGTTGCGTAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTg < 1:979331/66‑1 (MQ=255) tACCTGGCACCGTATGCCGGTTGCGTAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTg < 1:706733/66‑1 (MQ=255) tACCTGGCACCGTATGCCGGTTGCGTAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTg < 1:4868218/66‑1 (MQ=255) tACCTGGCACCGTATGCCGGTTGCGTAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTg < 1:4563500/66‑1 (MQ=255) tACCTGGCACCGTATGCCGGTTGCGTAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTg < 1:4347071/66‑1 (MQ=255) tACCTGGCACCGTATGCCGGTTGCGTAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTg < 1:4341307/66‑1 (MQ=255) tACCTGGCACCGTATGCCGGTTGCGTAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTg < 1:3008784/66‑1 (MQ=255) tACCTGGCACCGTATGCCGGTTGCGTAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTg < 1:2660159/66‑1 (MQ=255) tACCTGGCACCGTATGCCGGTTGCGTAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTg < 1:2542850/66‑1 (MQ=255) tACCTGGCACCGTATGCCGGTTGCGTAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTg < 1:2437666/66‑1 (MQ=255) tACCTGGCACCGTATGCCGGTTGCGTAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTg < 1:1728518/66‑1 (MQ=255) tACCTGGCACCGTATGCCGGTTGCGTAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTg < 1:2443425/66‑1 (MQ=255) tACCTGGCACCGTATGCCGGTTGCGTAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTg < 1:1486615/66‑1 (MQ=255) tACCTGGCACCGTATGCCGGTTGCGTAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTg < 1:1642407/66‑1 (MQ=255) tACCTGGCACCGTATGCCGGTTGCGTAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGAGCTg < 1:3180580/66‑1 (MQ=255) cTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTGATCATTTa < 1:4443399/71‑1 (MQ=255) cTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTGATCATTTa < 1:3945580/71‑1 (MQ=255) cTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTGATCATTTa < 1:2813947/71‑1 (MQ=255) cTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTGATCATTTa < 1:663369/71‑1 (MQ=255) cTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTGATCATTTa < 1:1165230/71‑1 (MQ=255) tGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTGATCATTTa < 1:321735/70‑1 (MQ=255) tGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTGATCATTTa < 1:3105614/70‑1 (MQ=255) gCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTGATCATTTAc < 1:2742767/69‑1 (MQ=255) gCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTGATCATTTAc < 1:2731593/69‑1 (MQ=255) gCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTGATCATTTAc < 1:4218849/69‑1 (MQ=255) gCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTGATCATTTAc < 1:2166410/69‑1 (MQ=255) gCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTGATCATTTAc < 1:4424797/69‑1 (MQ=255) gCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGATACGCTGATCATTTAc < 1:873812/69‑1 (MQ=255) agTATGCCGGTTGGGTAATGGGCGAATACTTCCGTGACCGCGGTGAAGTTGCGCTg < 1:2278738/55‑1 (MQ=255) tATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTGa < 1:1478077/55‑1 (MQ=255) tATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTGa < 1:782357/55‑1 (MQ=255) tATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTGa < 1:894383/55‑1 (MQ=255) tATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTGa < 1:4915962/55‑1 (MQ=255) tATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTGa < 1:1847318/55‑1 (MQ=255) tATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTGa < 1:4152405/55‑1 (MQ=255) tATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTGa < 1:4018917/55‑1 (MQ=255) tATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTGa < 1:3268450/55‑1 (MQ=255) tATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTGa < 1:3032318/55‑1 (MQ=255) tATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTGa < 1:2248643/55‑1 (MQ=255) aTGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTGa < 1:667803/54‑1 (MQ=255) ccGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTGATCATTTACGATGACCTGTc > 1:4351282/1‑70 (MQ=255) ccGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTGATCATTTACGATGACCTGTc > 1:2146320/1‑70 (MQ=255) ccGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTGATCATTTACGATGACCTGTc > 1:4138375/1‑70 (MQ=255) ccGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTGATCATTTACGATGACCTGTc > 1:3588145/1‑70 (MQ=255) ccGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTGATCATTTACGATGACCTGTc > 1:3380802/1‑70 (MQ=255) ccGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTGATCATTTACGATGACCTGTc > 1:3060482/1‑70 (MQ=255) ccGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTGATCATTTACGATGACCTGTc > 1:896705/1‑70 (MQ=255) ccGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTGATCATTTACGATGACCTGTc > 1:2931581/1‑70 (MQ=255) | ACCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTGATCATTTACGATGACCTGTC > minE/2467197‑2467327 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |