Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | minE | 460,687 | 0 | G | T | 39.3% | 113.1 / 79.3 | 84 | T79T (ACC→ACA) | gltA | citrate synthase |
Reads supporting (aligned to +/- strand): ref base G (14/37); new base T (0/33); total (14/70) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 5.59e-04 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.25e-01 | |||||||||||
Rejected as consensus: Frequency below/above cutoff threshold. |
GTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCAAAATACCTTCATCACCATCAATAAAAG > minE/460618‑460751 | gTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCg < 1:3893050/70‑1 (MQ=255) gTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCg < 1:4536433/70‑1 (MQ=255) gTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCg < 1:4850024/70‑1 (MQ=255) tCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGt < 1:1006650/71‑1 (MQ=255) tCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGt < 1:341734/71‑1 (MQ=255) tCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGt < 1:2802170/71‑1 (MQ=255) tCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGt < 1:4518723/71‑1 (MQ=255) tCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGt < 1:2325135/71‑1 (MQ=255) tCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGt < 1:1024438/71‑1 (MQ=255) ttCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAg < 1:3628715/69‑1 (MQ=255) ttCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAg < 1:2179323/69‑1 (MQ=255) ttCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAg < 1:3200425/69‑1 (MQ=255) ttCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAg < 1:4165846/69‑1 (MQ=255) ttCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAg < 1:3017261/69‑1 (MQ=255) ttCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAg < 1:2941349/69‑1 (MQ=255) ttCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAg < 1:2075907/69‑1 (MQ=255) ttCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAg < 1:2009439/69‑1 (MQ=255) agGATGTAACAAACTTGCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCaa < 1:2895807/71‑1 (MQ=255) agGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTgatcga > 1:2775213/1‑49 (MQ=255) agGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTgatcga > 1:281416/1‑49 (MQ=255) agGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTgatcga > 1:2809797/1‑49 (MQ=255) agGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTgatcga > 1:4027819/1‑49 (MQ=255) agGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTgatcga > 1:836456/1‑49 (MQ=255) agGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTgatcga > 1:270480/1‑49 (MQ=255) agGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTgatcga > 1:4405844/1‑49 (MQ=255) agGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTgatcga > 1:913579/1‑49 (MQ=255) agGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTgatcga > 1:1833985/1‑49 (MQ=255) agGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTgatcga > 1:1786772/1‑49 (MQ=255) agGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTgatcga > 1:668493/1‑49 (MQ=255) agGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCaa < 1:905516/71‑1 (MQ=255) agGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCaa < 1:2296151/71‑1 (MQ=255) agGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCaa < 1:3504066/71‑1 (MQ=255) agGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCaa < 1:565414/71‑1 (MQ=255) ggATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCaa < 1:4262059/70‑1 (MQ=255) aCAAACTTCCAGGTAGTTAGAATCTGTCGCCAGCTGATCGATCGGGAAACcgc < 1:834369/53‑1 (MQ=255) aCAAACTTCCAGGTAGTTAGAATCTGTCGCCAGCTGATCGATCGGGAAACcgc < 1:3655453/53‑1 (MQ=255) aCAAACTTCCAGGTAGTTAGAATCTGTCGCCAGCTGATCGATCGGGAAACcgc < 1:3741473/53‑1 (MQ=255) aCAAACTTCCAGGTAGTTAGAATCTGTCGCCAGCTGATCGATCGGGAAACcgc < 1:1008504/53‑1 (MQ=255) aCAAACTTCCAGGTAGTTAGAATCTGTCGCCAGCTGATCGATCGGGAAACcgc < 1:819743/53‑1 (MQ=255) aCAAACTTCCAGGTAGTTAGAATCTGTCGCCAGCTGATCGATCGGGAAACcgc < 1:3960997/53‑1 (MQ=255) aCAAACTTCCAGGTAGTTAGAATCTGTCGCCAGCTGATCGATCGGGAAACcgc < 1:4087875/53‑1 (MQ=255) aCAAACTTCCAGGTAGTTAGAATCTGTCGCCAGCTGATCGATCGGGAAACcgc < 1:4140112/53‑1 (MQ=255) aCAAACTTCCAGGTAGTTAGAATCTGTCGCCAGCTGATCGATCGGGAAACcgc < 1:870237/53‑1 (MQ=255) aCAAACTTCCAGGTAGTTAGAATCTGTCGCCAGCTGATCGATCGGGAAACcgc < 1:4795856/53‑1 (MQ=255) aCAAACTTCCAGGTAGTTAGAATCTGTCGCCAGCTGATCGATCGGGAAACcgc < 1:4645289/53‑1 (MQ=255) aCAAACTTCCAGGTAGTTAGAATCTGTCGCCAGCTGATCGATCGGGAAACcgc < 1:4627196/53‑1 (MQ=255) aCAAACTTCCAGGTAGTTAGAATCTGTCGCCAGCTGATCGATCGGGAAACcgc < 1:942248/53‑1 (MQ=255) aCAAACTTCCAGGTAGTTAGAATCTGTCGCCAGCTGATCGATCGGGAAACcgc < 1:4268334/53‑1 (MQ=255) aCAAACTTCCAGGTAGTTAGAATCTGTCGCCAGCTGATCGATCGGGAAACcgc < 1:896165/53‑1 (MQ=255) aCAAACTTCCAGGTAGTTAGAATCTGTCGCCAGCTGATCGATCGGGAAACcgc < 1:449470/53‑1 (MQ=255) aCAAACTTCCAGGTAGTTAGAATCTGTCGCCAGCTGATCGATCGGGAAACcgc < 1:241045/53‑1 (MQ=255) aCAAACTTCCAGGTAGTTAGAATCTGTCGCCAGCTGATCGATCGGGAAACcgc < 1:1046426/53‑1 (MQ=255) aCAAACTTCCAGGTAGTTAGAATCTGTCGCCAGCTGATCGATCGGGAAACcgc < 1:1399144/53‑1 (MQ=255) aCAAACTTCCAGGTAGTTAGAATCTGTCGCCAGCTGATCGATCGGGAAACcgc < 1:1458364/53‑1 (MQ=255) aCAAACTTCCAGGTAGTTAGAATCTGTCGCCAGCTGATCGATCGGGAAACcgc < 1:1513779/53‑1 (MQ=255) aCAAACTTCCAGGTAGTTAGAATCTGTCGCCAGCTGATCGATCGGGAAACcgc < 1:1639355/53‑1 (MQ=255) aCAAACTTCCAGGTAGTTAGAATCTGTCGCCAGCTGATCGATCGGGAAACcgc < 1:1658330/53‑1 (MQ=255) aCAAACTTCCAGGTAGTTAGAATCTGTCGCCAGCTGATCGATCGGGAAACcgc < 1:1800171/53‑1 (MQ=255) aCAAACTTCCAGGTAGTTAGAATCTGTCGCCAGCTGATCGATCGGGAAACcgc < 1:2164462/53‑1 (MQ=255) aCAAACTTCCAGGTAGTTAGAATCTGTCGCCAGCTGATCGATCGGGAAACcgc < 1:2975992/53‑1 (MQ=255) aCAAACTTCCAGGTAGTTAGAATCTGTCGCCAGCTGATCGATCGGGAAACcgc < 1:3117565/53‑1 (MQ=255) aCAAACTTCCAGGTAGTTAGAATCTGTCGCCAGCTGATCGATCGGGAAACcgc < 1:3277669/53‑1 (MQ=255) aCAAACTTCCAGGTAGTTAGAATCTGTCGCCAGCTGATCGATCGGGAAACcgc < 1:31223/53‑1 (MQ=255) aCAAACTTCCAGGTAGTTAGAATCTGTCGACAGCTGATCGATCGGGAAACcgc < 1:3115148/53‑1 (MQ=255) aCAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCaaaa > 1:1406714/1‑65 (MQ=255) aCAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCaaaa > 1:2605822/1‑65 (MQ=255) aCAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCaaaa > 1:533828/1‑65 (MQ=255) cAAACTTCCAGGTAGTTAGAATCTGTCGCCAGCTGATCGATCGGGAAACcgc < 1:4259178/52‑1 (MQ=255) aaaCTTCCAGGTAGTTAGAATCTGTCGCCAGCTGATCGATCGGGAAACcgc < 1:3587375/51‑1 (MQ=255) aaaCTTCCAGGTAGTTAGAATCTGTCGCCAGCTGATCGATCGGGAAACcgc < 1:823338/51‑1 (MQ=255) tAGTTAGAATCGGTCGCCAGCTGATCGATCGTGAAACCGCGGTGCAGCAAAATACCTTCATCACCATc < 1:2578684/68‑1 (MQ=255) tAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCAAAATACCTTCATCACCATc < 1:3893899/68‑1 (MQ=255) tAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCAAAATACCTTCATCACCATc < 1:3600517/68‑1 (MQ=255) tAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCAAAATACCTTCATCACCATc < 1:1603910/68‑1 (MQ=255) tAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCAAAATACCTTCATCACCATc < 1:3311383/68‑1 (MQ=255) tAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCAAAATACCTTCATCACCATc < 1:3774604/68‑1 (MQ=255) tAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCAAAATACCTTCATCACCATc < 1:145409/68‑1 (MQ=255) tAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCAAAATACCTTCATCACCATc < 1:113859/68‑1 (MQ=255) tAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCAAAATACCTTCATCACCATc < 1:330898/68‑1 (MQ=255) tAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCAAAATACCTTCATCACCATc < 1:462972/68‑1 (MQ=255) tAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCAAAATACCTTCATCACCATc < 1:2170333/68‑1 (MQ=255) tAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCAAAATACCTTCATCACCATc < 1:2328706/68‑1 (MQ=255) tAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCAAAATACCTTCATCACCATc < 1:3604096/68‑1 (MQ=255) tAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCAAAATACCTTCATCACCATc < 1:437609/68‑1 (MQ=255) tAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCAAAATACCTTCATCACCATc < 1:2794740/68‑1 (MQ=255) tAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCAAAATACCTTCATCACCATc < 1:4263925/68‑1 (MQ=255) tAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCAAAATACCTTCATCACCATc < 1:4172283/68‑1 (MQ=255) ggTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCAAAATACCTTCATCACCATCAATAAAAg > 1:1657266/1‑65 (MQ=255) | GTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCAAAATACCTTCATCACCATCAATAAAAG > minE/460618‑460751 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |