| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 751,711 | 0 | G | T | 38.9% | 78.6 / 46.9 | 54 | G503G (GGC→GGA) | mfd | transcription‑repair coupling factor |
| Reads supporting (aligned to +/- strand): ref base G (14/19); new base T (3/18); total (17/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.82e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACGACACCGGAACATACAGTTTGGCGTCGTTGGCATAGGTGAGCATCAGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGACCACCGGCTG > minE/751647‑751779 | acgacACCGGAACATACAGTTTGGCGTCGTTGGCATAGGTGAGCATCAGATACTCGCCAGTAATGCCAccc > 1:1202331/1‑71 (MQ=255)acgacACCGGAACATACAGTTTGGCGTCGTTGGCATAGGTGAGCATCAGATACTCGCCAGTAATGCCAccc > 1:1672417/1‑71 (MQ=255)acgacACCGGAACATACAGTTTGGCGTCGTTGGCATAGGTGAGCATCAGATACTCGCCAGTAATGCCAcc > 1:3053338/1‑70 (MQ=255) gacACCGGAACATACAGTTTGGCGTCGTTGGCATAGGTGAGCATCAGATACTCGCCAGTAATGCCACCCGc > 1:1002911/1‑71 (MQ=255) gacACCGGAACATACAGTTTGGCGTCGTTGGCATAGGTGAGCATCAGATACTCGCCAGTAATGCCACCCGc < 1:2449378/71‑1 (MQ=255) gacACCGGAACATACAGTTTGGCGTCGTTGGCATAGGTGAGCATCAGATACTAGCCAGTAATGCCACCCGc < 1:1723131/71‑1 (MQ=255) aCAGTTTGGCGTCGTTGGCATAGGTGAGCATCAGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGt > 1:977607/1‑70 (MQ=255) aCAGTTTGGCGTCGTTGGCATAGGTGAGCATCAGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGt > 1:4779803/1‑70 (MQ=255) aCAGTTTGGCGTCGTTGGCATAGGTGAGCATCAGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGt > 1:4026749/1‑70 (MQ=255) aCAGTTTGGCGTCGTTGGCATAGGTGAGCATCAGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGt > 1:361780/1‑70 (MQ=255) aCAGTTTGGCGTCGTTGGCATAGGTGAGCATCAGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGt > 1:2599195/1‑70 (MQ=255) gAGCATCAGATACTCGCCAGTAATTCCACCCGCTTCCAGCTTGGTCa < 1:1076510/47‑1 (MQ=255) gAGCATCAGATACTCGCCAGTAATTCCACCCGCTTCCAGCGTGGTCa < 1:3311472/47‑1 (MQ=255) gAGCATCAGATACTCGCCAGTAATTCCACCCGCTTCCAGCGTGGTCa < 1:1114244/47‑1 (MQ=255) gAGCATCAGATACTCGCCAGTAATTCCACCCGCTTCCAGCGTGGTCa < 1:50375/47‑1 (MQ=255) gAGCATCAGATACTCGCCAGTAATTCCACCCGCTTCCAGCGTGGTCa < 1:1155806/47‑1 (MQ=255) gAGCATCAGATACTCGCCAGTAATTCCACCCGCTTCCAGCGTGGTCa < 1:4647979/47‑1 (MQ=255) gAGCATCAGATACTCGCCAGTAATTCCACCCGCTTCCAGCGTGGTCa < 1:4642229/47‑1 (MQ=255) gAGCATCAGATACTCGCCAGTAATTCCACCCGCTTCCAGCGTGGTCa < 1:4383195/47‑1 (MQ=255) gAGCATCAGATACTCGCCAGTAATTCCACCCGCTTCCAGCGTGGTCa < 1:4166740/47‑1 (MQ=255) gAGCATCAGATACTCGCCAGTAATTCCACCCGCTTCCAGCGTGGTCa < 1:4071779/47‑1 (MQ=255) gAGCATCAGATACTCGCCAGTAATTCCACCCGCTTCCAGCGTGGTCa < 1:2880734/47‑1 (MQ=255) gAGCATCAGATACTCGCCAGTAATTCCACCCGCTTCCAGCGTGGTCa < 1:2804716/47‑1 (MQ=255) gAGCATCAGATACTCGCCAGTAATTCCACCCGCTTCCAGCGTGGTCa < 1:2773948/47‑1 (MQ=255) gAGCATCAGATACTCGCCAGTAATTCCACCCGCTTCCAGCGTGGTCa < 1:2619848/47‑1 (MQ=255) gAGCATCAGATACTCGCCAGTAATTCCACCCGCTTCCAGCGTGGTCa < 1:2615521/47‑1 (MQ=255) gAGCATCAGATACTCGCCAGTAATTCCACCCGCTTCCAGCGTGGTCa < 1:2402989/47‑1 (MQ=255) aGCATCAGATACTCGCCAGTAATTCCACCCGCTTCCAGCGTGGTCa < 1:4334329/46‑1 (MQ=255) gCATCAGATACTCGCCAGTAATTCCACCCGCTTCCAGCGTGGTCAtt > 1:896102/1‑47 (MQ=255) gCATCAGATACTCGCCAGTAATTCCACCCGCTTCCAGCGTGGTCAtt > 1:3283436/1‑47 (MQ=255) gCATCAGATACTCGCCAGTAATTCCACCCGCTTCCAGCGTGGTCAtt > 1:2631215/1‑47 (MQ=255) aTCAGATACTCGCCAGTAATTCCACCCGCTTCCAGCGTGGTCa < 1:4909846/43‑1 (MQ=255) aGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCa < 1:1313560/70‑1 (MQ=255) aGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCa < 1:4341339/70‑1 (MQ=255) aGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCa < 1:2547301/70‑1 (MQ=255) aGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCa < 1:1475085/70‑1 (MQ=255) aGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCa < 1:3974284/70‑1 (MQ=255) aGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCa < 1:3810858/70‑1 (MQ=255) aGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCa < 1:137072/70‑1 (MQ=255) aGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCa < 1:3567725/70‑1 (MQ=255) aGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCa < 1:2836527/70‑1 (MQ=255) aGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCa < 1:1812588/70‑1 (MQ=255) aGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCa < 1:3291193/70‑1 (MQ=255) aTACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGAt > 1:1384383/1‑71 (MQ=255) aTACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGAt > 1:2927602/1‑71 (MQ=255) aTACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGAt > 1:2355635/1‑71 (MQ=255) aTACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGAt > 1:3027410/1‑71 (MQ=255) aTACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGAt > 1:3844292/1‑71 (MQ=255) cTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATgg < 1:2315338/70‑1 (MQ=255) aTGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGACCACCGGCTg < 1:149477/71‑1 (MQ=255) aTGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGACCACCGGCTg < 1:432029/71‑1 (MQ=255) aTGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGACCACCGGCTg < 1:2117456/71‑1 (MQ=255) aTGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGACCACCGGCTg < 1:4124504/71‑1 (MQ=255) aTGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGACCACCGGCTg < 1:1946577/71‑1 (MQ=255) | ACGACACCGGAACATACAGTTTGGCGTCGTTGGCATAGGTGAGCATCAGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGACCACCGGCTG > minE/751647‑751779 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |