Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	505,424	T→C	G128G (GGT→GGC)	ybhE →	6‑phosphogluconolactonase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	505,424	0	T	C	100.0%	139.2 / NA	38	G128G (GGT→GGC)	ybhE	6‑phosphogluconolactonase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (5/33);  total (5/33)

CGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCG  >  minE/505366‑505489                                                           |                                                                  cgcgTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGCTGCCATTCCGcc                                                       <  1:4917966/71‑1 (MQ=255) cgcgTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGCTGCCATTCCGcc                                                       <  1:4662851/71‑1 (MQ=255)       tGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGCTGCCATTCCGcc                                                       <  1:3400886/65‑1 (MQ=255)       tGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGCTGCCATTCCGcc                                                       <  1:4315342/65‑1 (MQ=255)       tGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGCTGCCATTCCGcc                                                       <  1:4031363/65‑1 (MQ=255)       tGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGCTGCCATTCCGcc                                                       <  1:2294248/65‑1 (MQ=255)       tGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGCTGCCATTCCGCCAATAtc                                                 >  1:838281/1‑71 (MQ=255)       tGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGCTGCCATTCCGCCAATAtc                                                 >  1:3577657/1‑71 (MQ=255)        ggAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGCTGCCATTCCGcc                                                       <  1:3029947/64‑1 (MQ=255)         taaGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGCTGCCATTCCGCCAATAtctc                                               <  1:1818357/70‑1 (MQ=255)         gAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGCTGCCATTCCGCCAATAtctc                                               <  1:1856577/71‑1 (MQ=255)         gAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGCTGCCATTCCGCCAATAtctc                                               <  1:2907878/71‑1 (MQ=255)         gAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGCTGCCATTCCGCCAATAtctc                                               <  1:2240042/71‑1 (MQ=255)         gAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGCTGCCATTCCGCCAATAtctc                                               <  1:4097800/71‑1 (MQ=255)         gAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGCTGCCATTCCGCCAATAtctc                                               <  1:1990072/71‑1 (MQ=255)         gAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGCTGCCATTCCGCCAATAtctc                                               <  1:4154927/71‑1 (MQ=255)         gAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGCTGCCATTCCGCCAATAtctc                                               <  1:728170/71‑1 (MQ=255)         gAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGCTGCCATTCCGCCAATAtctc                                               <  1:1358738/71‑1 (MQ=255)         gAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGCTGCCATTCCGCCAATAtctc                                               <  1:1005542/71‑1 (MQ=255)               ggCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGCTGCCATTCCGCCAATAtctc                                               <  1:1247236/65‑1 (MQ=255)                 ccTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGCTGCCATTCCGCCAat                                                    <  1:1601017/58‑1 (MQ=255)                   tGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGCTGCCATTcc                                                          >  1:3205062/1‑50 (MQ=255)                   tGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGCTGCCATTCCGCCAatat                                                  >  1:716091/1‑58 (MQ=255)                   tGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGCTGCCATTCCGCCAatat                                                  >  1:4063910/1‑58 (MQ=255)                     ccAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGCTGCCATTCCGCCAATATCTCACCGGACAACCg                                   <  1:1786657/71‑1 (MQ=255)                             cgtcgtCGATGTGGTCGAGGGGCTGGACGGCTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCtgt                           <  1:3673094/71‑1 (MQ=255)                             cgtcgtCGATGTGGTCGAGGGGCTGGACGGCTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCtgt                           <  1:60895/71‑1 (MQ=255)                             cgtcgtCGATGTGGTCGAGGGGCTGGACGGCTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCtgt                           <  1:2010326/71‑1 (MQ=255)                             cgtcgtCGATGTGGTCGAGGGGCTGGACGGCTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCtgt                           <  1:4155230/71‑1 (MQ=255)                                 gtcgATGTGGTCGAGGGGCTGGACGGCTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGt                       <  1:1308977/71‑1 (MQ=255)                                 gtcgATGTGGTCGAGGGGCTGGACGGCTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGt                       <  1:3239708/71‑1 (MQ=255)                                           tCGAGGGGCTGGACGGCTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTa             <  1:431456/71‑1 (MQ=255)                                           tCGAGGGGCTGGACGGCTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTa             <  1:1802508/71‑1 (MQ=255)                                           tCGAGGGGCTGGACGGCTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTa             <  1:1633096/71‑1 (MQ=255)                                           tCGAGGGGCTGGACGGCTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTa             <  1:2521539/71‑1 (MQ=255)                                           tCGAGGGGCTGGACGGCTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTa             <  1:3067738/71‑1 (MQ=255)                                                      gACGGCTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCg  <  1:1809295/71‑1 (MQ=255)                                                      gACGGCTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCg  <  1:2533347/71‑1 (MQ=255)                                                           |                                                                  CGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCG  >  minE/505366‑505489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑