BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	518,289	G→A	D105N (GAT→AAT)	bioC →	predicted methltransferase, enzyme of biotin synthesis

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	518,289	0	G	A	100.0%	365.0 / NA	110	D105N (GAT→AAT)	bioC	predicted methltransferase, enzyme of biotin synthesis
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (55/55); total (55/55)

Only 100 of 110 total aligned reads displayed.

AGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCAC  >  minE/518224‑518338

aGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCAATCtt                                              >  1:3701688/1‑71 (MQ=255)

   aTGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCAATCTTGCa                                           <  1:1716209/71‑1 (MQ=255)

    tGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCAATCTTGCAt                                          >  1:777208/1‑71 (MQ=255)

    tGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCAATCTTGCAt                                          >  1:4662558/1‑71 (MQ=255)

    tGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCAATCTTGCAt                                          >  1:3970050/1‑71 (MQ=255)

    tGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCAATCTTGCAt                                          >  1:183235/1‑71 (MQ=255)

                ttATCTGGCGGGAGATATCGAATCCGTGCCGTTAGCGACTGCGACGTTCAATCt                                               <  1:1082167/54‑1 (MQ=39)

                ttATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCAATCt                                               <  1:2616134/54‑1 (MQ=255)

                ttATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCAATCt                                               <  1:2426360/54‑1 (MQ=255)

                ttATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCAATCt                                               <  1:2878805/54‑1 (MQ=255)

                ttATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCAATCt                                               <  1:3204581/54‑1 (MQ=255)

                ttATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCAATCt                                               <  1:3435874/54‑1 (MQ=255)

                ttATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCAATCt                                               <  1:4337427/54‑1 (MQ=255)

                ttATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCAATCt                                               <  1:1587681/54‑1 (MQ=255)

                ttATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCAATCt                                               <  1:1403871/54‑1 (MQ=255)

                ttATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCAATCt                                               <  1:1175988/54‑1 (MQ=255)

                 tATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCAATCt                                               <  1:4600837/53‑1 (MQ=255)

                  aTCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCgc                             <  1:3918551/70‑1 (MQ=255)

                  aTCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCgc                             <  1:143530/70‑1 (MQ=255)

                  aTCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCgc                             <  1:2274835/70‑1 (MQ=255)

                  aTCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCgc                             <  1:3050929/70‑1 (MQ=255)

                  aTCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCgc                             <  1:2719402/70‑1 (MQ=255)

                  aTCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCgc                             <  1:2624256/70‑1 (MQ=255)

                   tCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCgc                             <  1:4581745/69‑1 (MQ=255)

                   tCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCgc                             <  1:875549/69‑1 (MQ=255)

                     tGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCgcagtg                         >  1:4122470/1‑71 (MQ=255)

                     tGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCgcagtg                         >  1:4647826/1‑71 (MQ=255)

                     tGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCgcagtg                         >  1:476889/1‑71 (MQ=255)

                     tGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCgcagtg                         >  1:927143/1‑71 (MQ=255)

                             gaTATCGAATCCCTGCCGTTAGCGACTGCGACGTTCAAt                                                 >  1:4746848/1‑39 (MQ=255)

                             gaTATCGAATCCCTGCCGTTAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAgtggtg                 >  1:1059006/1‑71 (MQ=255)

                             gaTATCGAATCCCTGCCGTTAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAgtggtg                 >  1:3671030/1‑71 (MQ=255)

                             gaTATCGAATCCCTGCCGTTAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAgtggtg                 >  1:3409882/1‑71 (MQ=255)

                                 tCGAATCCCTGCCGTTAGCGACTGCGACGTTCAATCTTGCATGGAGCa                                    <  1:541495/48‑1 (MQ=255)

                                 tCGAATCCCTGCCGTTAGCGACTGCGACGTTCAATCTTGCATGGAGCa                                    <  1:4603838/48‑1 (MQ=255)

                                 tCGAATCCCTGCCGTTAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCgg              >  1:2084650/1‑70 (MQ=255)

                                 tCGAATCCCTGCCGTTAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCgg              >  1:1134482/1‑70 (MQ=255)

                                 tCGAATCCCTGCCGTTAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCgg              >  1:1723574/1‑70 (MQ=255)

                                 tCGAATCCCTGCCGTTAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCgg              >  1:419223/1‑70 (MQ=255)

                                 tCGAATCCCTGCCGTTAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCgg              >  1:2035465/1‑70 (MQ=255)

                                 tCGAATCCCTGCCGTTAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCgg              >  1:2622662/1‑70 (MQ=255)

                                 tCGAATCCCTGCCGTTAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCg               >  1:2557616/1‑69 (MQ=255)

                                      tCCCTGCCGTTAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAAtt         >  1:3693850/1‑70 (MQ=255)

                                      tCCCTGCCGTTAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAAtt         >  1:923864/1‑70 (MQ=255)

                                      tCCCTGCCGTTAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAAtt         >  1:1273360/1‑70 (MQ=255)

                                      tCCCTGCCGTTAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAAtt         >  1:3769084/1‑70 (MQ=255)

                                      tCCCTGCCGTTAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAAtt         >  1:4517248/1‑70 (MQ=255)

                                      tCCCTGCCGTTAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAAtt         >  1:2127055/1‑70 (MQ=255)

                                      tCCCTGCCGTTAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAAtt         >  1:1725112/1‑70 (MQ=255)

                                      tCCCTGCCGTTAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAAtt         >  1:2667753/1‑70 (MQ=255)

                                      tCCCTGCCGTTAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAAtt         >  1:272668/1‑70 (MQ=255)

                                            ccGTTAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATcc    >  1:352206/1‑69 (MQ=255)

                                            ccGTTAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCa   <  1:4397267/70‑1 (MQ=255)

                                            ccGTTAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCa   <  1:4191183/70‑1 (MQ=255)

                                            ccGTTAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCa   <  1:2533207/70‑1 (MQ=255)

                                            ccGTTAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCa   <  1:913929/70‑1 (MQ=255)

                                            ccGTTAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCa   >  1:1575612/1‑70 (MQ=255)

                                            ccGTTAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCAc  >  1:2354828/1‑71 (MQ=255)

                                            ccGTTAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCAc  >  1:2521404/1‑71 (MQ=255)

                                            ccGTTAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCAc  >  1:2485926/1‑71 (MQ=255)

                                            ccGTTAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCAc  >  1:2471146/1‑71 (MQ=255)

                                            ccGTTAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCAc  >  1:2893585/1‑71 (MQ=255)

                                            ccGTTAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCAc  >  1:311055/1‑71 (MQ=255)

                                            ccGTTAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCAc  >  1:3355452/1‑71 (MQ=255)

                                            ccGTTAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCAc  >  1:3244789/1‑71 (MQ=255)

                                            ccGTTAGCGACTGCGACCTTCAATCATGCATGGAGCAATCTCGCAGACCAGAGGTGCGGTAATTTATCCAc  >  1:2235550/1‑71 (MQ=255)

                                             cGTTAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCa   <  1:371631/69‑1 (MQ=255)

                                               ttAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATc     <  1:125161/65‑1 (MQ=255)

                                               ttAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATc     <  1:843614/65‑1 (MQ=255)

                                               ttAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATc     <  1:687672/65‑1 (MQ=255)

                                               ttAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATc     <  1:4883971/65‑1 (MQ=255)

                                               ttAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATc     <  1:3429089/65‑1 (MQ=255)

                                               ttAGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATc     <  1:85634/65‑1 (MQ=255)

                                                 aGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCa   >  1:3688285/1‑65 (MQ=255)

                                                 aGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCa   >  1:2949229/1‑65 (MQ=255)

                                                 aGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCa   >  1:3083209/1‑65 (MQ=255)

                                                 aGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCa   >  1:4348174/1‑65 (MQ=255)

                                                 aGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCa   >  1:55841/1‑65 (MQ=255)

                                                 aGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCa   >  1:2811172/1‑65 (MQ=255)

                                                 aGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCa   >  1:7011/1‑65 (MQ=255)

                                                 aGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCa   >  1:708027/1‑65 (MQ=255)

                                                 aGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCa   >  1:3606179/1‑65 (MQ=255)

                                                 aGCGACTGCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCa   >  1:3633546/1‑65 (MQ=255)

                                                        gCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTa            <  1:4107781/49‑1 (MQ=255)

                                                        gCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTa            <  1:416562/49‑1 (MQ=255)

                                                        gCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTa            <  1:3460077/49‑1 (MQ=255)

                                                        gCGACGTTCAATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTa            <  1:3358475/49‑1 (MQ=255)

                                                              ttCAATGTTGCATGGAGCAATCTCGCAGTGCAgtggt                  <  1:4392013/37‑1 (MQ=38)

                                                              ttCAATCTTGCATGGAGCAATCTCGCAGTGCAgtggt                  <  1:132056/37‑1 (MQ=255)

                                                              ttCAATCTTGCATGGAGCAATCTCGCAGTGCAgtggt                  <  1:1462564/37‑1 (MQ=255)

                                                              ttCAATCTTGCATGGAGCAATCTCGCAGTGCAgtggt                  <  1:2443129/37‑1 (MQ=255)

                                                              ttCAATCTTGCATGGAGCAATCTCGCAGTGCAgtggt                  <  1:1882422/37‑1 (MQ=255)

                                                              ttCAATCTTGCATGGAGCAATCTCGCAGTGCAgtggt                  <  1:1983661/37‑1 (MQ=255)

                                                              ttCAATCTTGCATGGAGCAATCTCGCAGTGCAgtggt                  <  1:2701904/37‑1 (MQ=255)

                                                              ttCAATCTTGCATGGAGCAATCTCGCAGTGCAgtggt                  <  1:3866388/37‑1 (MQ=255)

                                                              ttCAATCTTGCATGGAGCAATCTCGCAGTGCAgtggt                  <  1:3525834/37‑1 (MQ=255)

                                                              ttCAATCTTGCATGGAGCAATCTCGCAGTGCAgtggt                  <  1:3123874/37‑1 (MQ=255)

                                                              ttCAATCTTGCATGGAGCAATCTCGCAGTGCAgtggt                  <  1:3098001/37‑1 (MQ=255)

                                                              ttCAATCTTGCATGGAGCAATCTCGCAGTGCAgtggt                  <  1:2285376/37‑1 (MQ=255)

                                                              ttCAATCTTGCATGGAGCAATCTCGCAGTGCAgtggt                  <  1:2314541/37‑1 (MQ=255)

AGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCAC  >  minE/518224‑518338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`