BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	788,731	T→C	N242S (AAC→AGC)	ycgB ←	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	788,731	0	T	C	100.0%	121.9 / NA	33	N242S (AAC→AGC)	ycgB	conserved hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (21/12); total (21/12)

GGATTTCACGCTGCCATGATTCCAGCAGTGGCGCATTTTTCTCCATAAAATAGAGCAGGTTTTCTTGTGGTTCGGACGGATAGCGGCGCGCTTCAGCAACCGTTTTCTCTTCCTCGCGCTTC  >  minE/788672‑788793

ggATTTCACGCTGCCATGATTCCAGCAGTGGCGCATTTTTCTCCATAAAATAGAGCAGGCTTTCTTGTGGt                                                     >  1:4928907/1‑71 (MQ=255)

ggATTTCACGCTGCCATGATTCCAGCAGTGGCGCATTTTTCTCCATAAAATAGAGCAGGCTTTCTTGTGGt                                                     >  1:2309175/1‑71 (MQ=255)

   tttCACGCTGCCATGATTCCAGCAGTGGCGCATTTTTCTCCATAAAATAGAGCAGGCTTTTTTGTGGTTcg                                                  <  1:342853/71‑1 (MQ=255)

   tttCACGCTGCCATGATTCCAGCAGTGGCGCATTTTTCTCCATAAAATAGAGCAGGCTTTCTTGTGGTTcg                                                  <  1:4843161/71‑1 (MQ=255)

   tttCACGCTGCCATGATTCCAGCAGTGGCGCATTTTTCTCCATAAAATAGAGCAGGCTTTCTTGTGGTTcg                                                  <  1:439140/71‑1 (MQ=255)

   tttCACGCTGCCATGATTCCAGCAGTGGCGCATTTTTCTCCATAAAATAGAGCAGGCTTTCTTGTGGTTcg                                                  <  1:3562430/71‑1 (MQ=255)

    ttCACGCTGCCATGATTCCAGCAGTGGCGCATTTTTCTCCATAAAATAGAGCAGGCTTTCTTGTGGTTcg                                                  <  1:100600/70‑1 (MQ=255)

          cTGCCATGATTCCAGCAGTGGCGCATTTTTCTCCATAAAATAGAGCAGGCTTTCTTGTGGTTcggacgga                                            >  1:1243848/1‑70 (MQ=255)

          cTGCCATGATTCCAGCAGTGGCGCATTTTTCTCCATAAAATAGAGCAGGCTTTCTTGTGGTTcggacgga                                            >  1:818273/1‑70 (MQ=255)

          cTGCCATGATTCCAGCAGTGGCGCATTTTTCTCCATAAAATAGAGCAGGCTTTCTTGTGGTTcggacgga                                            >  1:418032/1‑70 (MQ=255)

          cTGCCATGATTCCAGCAGTGGCGCATTTTTCTCCATAAAATAGAGCAGGCTTTCTTGTGGTTcggacgga                                            >  1:4094631/1‑70 (MQ=255)

          cTGCCATGATTCCAGCAGTGGCGCATTTTTCTCCATAAAATAGAGCAGGCTTTCTTGTGGTTcggacgga                                            >  1:1260553/1‑70 (MQ=255)

          cTGCCATGATTCCAGCAGTGGCGCATTTTTCTCCATAAAATAGAGCAGGCTTTCTTGTGGTAcggacgga                                            >  1:3070424/1‑70 (MQ=255)

              cATGATTCCAGCAGTGGCGCATTTTTCTCCATAAAATAGAGCAGGCTTTCTTGTGGTTcg                                                  <  1:376140/60‑1 (MQ=255)

                                 cATTTTTCTCCATAAAATAGAGCAGGCTTTCTTGTGGTTCGGACGGATAGCGGCGCGCTTCAGCAACCGtt                    <  1:1520107/71‑1 (MQ=255)

                                  aTTTTTCTCCATAAAATAGAGCAGGCTTTCTTGTGGTTCGGACGGATAGCGGCGCGCTTCAGCAACCGttt                   >  1:2882206/1‑71 (MQ=255)

                                   tttttCTCCATAAAATAGAGCAGGCTTTCTTGTGGTTCGGACGGATAGCGGCGCGCTTCAGCAACCGtttt                  >  1:4266365/1‑71 (MQ=255)

                                   tttttCTCCATAAAATAGAGCAGGCTTTCTTGTGGTTCGGACGGATAGCGGCGCGCTTCAGCAACCGtttt                  >  1:4074697/1‑71 (MQ=255)

                                   tttttCTCCATAAAATAGAGCAGGCTTTCTTGTGGTTCGGACGGATAGCGGCGCGCTTCAGCAACCGtttt                  >  1:3579126/1‑71 (MQ=255)

                                   tttttCTCCATAAAATAGAGCAGGCTTTCTTGTGGTTCGGACGGATAGCGGCGCGCTTCAGCAACCGtttt                  >  1:2926419/1‑71 (MQ=255)

                                   tttttCTCCATAAAATAGAGCAGGCTTTCTTGTGGTTCGGACGGATAGCGGCGCGCTTCAGCAACCGttt                   >  1:640687/1‑70 (MQ=255)

                                     tttCTCCATAAAATAGAGCAGGCTTTCTTGTGGTTCGGACGGATAGCGGCGCGCTTCAGCAACCGTTTtc                 <  1:4765494/70‑1 (MQ=255)

                                                aaTAGAGCAGGCTTTCTTGTGGTTCGGACGGATAGCGGCGCGCTTCAGCAACCGTTTTCTCTTCCTcgctc     <  1:4791721/71‑3 (MQ=255)

                                                aaTAGAGCAGGCTTTCTTGTGGTTCGGACGGATAGCGGCGCGCTTCAGCAACCGTTTTCTCTTCCTCgcgc     <  1:4746557/71‑1 (MQ=255)

                                                aaTAGAGCAGGCTTTCTTGTGGTTCGGACGGATAGCGGCGCGCTTCAGCAACCGTTTTCTCTTCCTCgcgc     <  1:4860577/71‑1 (MQ=255)

                                                aaTAGAGCAGGCTTTCTTGTGGTTCGGACGGATAGCGGCGCGCTTCAGCAACCGTTTTCTCTTCCTCgcgc     <  1:3355413/71‑1 (MQ=255)

                                                   agagCAGGCTTTCTTGTGGTTCGGACGGATAGCGGCGCGCTTCAGCAACCGTTTTCTCTTCCTCGCGCt    >  1:4414737/1‑69 (MQ=255)

                                                   agagCAGGCTTTCTTGTGGTTCGGACGGATAGCGGCGCGCTTCAGCAACCGTTTTCTCTTCCTCGCGCTTc  >  1:3695273/1‑71 (MQ=255)

                                                   agagCAGGCTTTCTTGTGGTTCGGACGGATAGCGGCGCGCTTCAGCAACCGTTTTCTCTTCCTCGCGCTTc  >  1:1693132/1‑71 (MQ=255)

                                                   agagCAGGCTTTCTTGTGGTTCGGACGGATAGCGGCGCGCTTCAGCAACCGTTTTCTCTTCCTCGCGCTTc  >  1:329942/1‑71 (MQ=255)

                                                   agagCAGGCTTTCTTGTGGTTCGGACGGATAGCGGCGCGCTTCAGCAACCGTTTTCTCTTCCTCGCGCTTc  >  1:3486639/1‑71 (MQ=255)

                                                   agagCAGGCTTTCTTGTGGTTCGGACGGATAGCGGCGCGCTTCAGCAACCGTTTTCTCTTCCTCGCGCTTc  >  1:1481155/1‑71 (MQ=255)

                                                   agagCAGGCTTTCTTGTGGTTCGGACGGATAGCGGCGCGCTTCAGCAACCGTTTTCTCTTCCTCGCGCTTc  >  1:1363711/1‑71 (MQ=255)

GGATTTCACGCTGCCATGATTCCAGCAGTGGCGCATTTTTCTCCATAAAATAGAGCAGGTTTTCTTGTGGTTCGGACGGATAGCGGCGCGCTTCAGCAACCGTTTTCTCTTCCTCGCGCTTC  >  minE/788672‑788793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`