Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	793,951	G→A	A112V (GCG→GTG)	cvrA ←	predicted cation/proton antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	793,951	0	G	A	100.0%	175.8 / NA	55	A112V (GCG→GTG)	cvrA	predicted cation/proton antiporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/20);  total (35/20)

AGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGT  >  minE/793893‑794010                                                           |                                                            agagCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACACCGCCATCATGc                                                 >  1:3050228/1‑71 (MQ=255) agagCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACACCGCCATCATGc                                                 >  1:3595611/1‑71 (MQ=255) agagCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACACCGCCATCATGc                                                 >  1:1381118/1‑71 (MQ=255) agagCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACACCGCCATCATGc                                                 >  1:4773038/1‑71 (MQ=255) agagCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACACCGCCATCATGc                                                 >  1:67287/1‑71 (MQ=255)     ccGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACACCGCCATCATGCCgg                                              >  1:2221405/1‑70 (MQ=255)     ccGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACACCGCCATCATGCCGGt                                             >  1:4132675/1‑71 (MQ=255)     ccGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACACCGCCATCATGCCGGt                                             >  1:2445643/1‑71 (MQ=255)     ccGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACACCGCCATCATGCCGGt                                             >  1:1252194/1‑71 (MQ=255)             aGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACACCGCCATCATGCCGGTTAAAc                                        >  1:672014/1‑68 (MQ=255)             aGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACACCGCCATCATGCCGGTTAAAc                                        >  1:1231296/1‑68 (MQ=255)             aGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACACCGCCATCATGCCGGTTAAAc                                        >  1:44506/1‑68 (MQ=255)                gcCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACACCGCCATCATGCCGGTTAAACCAGAgg                                  >  1:1519580/1‑71 (MQ=255)                gcCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACACCGCCATCATGCCGGTTAAACCAGAgg                                  >  1:427601/1‑71 (MQ=255)                gcCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACACCGCCATCATGCCGGTTAAACCAGAgg                                  >  1:18333/1‑71 (MQ=255)                gcCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACACCGCCATCATGCCGGTTAAACCAGAgg                                  >  1:488579/1‑71 (MQ=255)                gcCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACACCGCCATCATGCCGGTTAAACCAGAgg                                  >  1:1224561/1‑71 (MQ=255)                 ccGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACACCGCCATCATGCCGGTTAAACCAGAGGt                                 >  1:1082738/1‑71 (MQ=255)                 ccGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACACCGCCATCATGCCGGTTAAACCAGAGGt                                 >  1:4392754/1‑71 (MQ=255)                 ccGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACACCGCCATCATGCCGGTTAAACCAGAGGt                                 >  1:2596701/1‑71 (MQ=255)                   gATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACACCGCCATCATGCCGGTTAAACCAGAGGTGa                               <  1:2616985/71‑1 (MQ=255)                   gATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACACCGCCATCATGCCGGTTAAACCAGAGGTGa                               <  1:3768114/71‑1 (MQ=255)                     ttAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACACCGCCATCATGCCGGTTAAACCAGAGGTGATa                             <  1:2485502/71‑1 (MQ=255)                     ttAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACACCGCCATCATGCCGGTTAAACCAGAGGTGATa                             <  1:450920/71‑1 (MQ=255)                     ttAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACACCGCCATCATGCCGGTTAAACCAGAGGTGATa                             <  1:677248/71‑1 (MQ=255)                     ttAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACACCGCCATCATGCCGGTTAAACCAGAGGTGATa                             <  1:3883622/71‑1 (MQ=255)                     ttAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACACCGCCATCATGCCGGTTAAACCAGAGGTGATa                             <  1:2061809/71‑1 (MQ=255)                     ttAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACACCGCCATCATGCCGGTTAAACCAGAGGTGATa                             <  1:3786962/71‑1 (MQ=255)                     ttAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACACCGCCATCATGCCGGTTAAACCAGAGGTGATa                             <  1:2073722/71‑1 (MQ=255)                     ttAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACACCGCCATCATGCCGGTTAAACCAGAGGTGATa                             <  1:4905679/71‑1 (MQ=255)                     ttAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACACCGCCATCATGCCGGTTAAACCAGAGGTGATa                             <  1:2359581/71‑1 (MQ=255)                      taataaGCCTTCAATCAAATCAAGATTAAACAGCCACACCGCCATCATGCCGGTTAAACCAGAGGTGAt                              >  1:3750130/1‑69 (MQ=255)                      taataaGCCTTCAATCAAATCAAGATTAAACAGCCACACCGCCATCATGCCGGTTAAACCAGAGGTGAt                              >  1:1758151/1‑69 (MQ=255)                      taataaGCCTTCAATCAAATCAAGATTAAACAGCCACACCGCCATCATGCCGGTTAAACCAGAGGTGAt                              >  1:4692935/1‑69 (MQ=255)                      taataaGCCTTCAATCAAATCAAGATTAAACAGCCACACCGCCATCATGCCGGTTAAACCAGAGGTGAt                              >  1:4889943/1‑69 (MQ=255)                      taataaGCCTTCAATCAAATCAAGATTAAACAGCCACACCGCCATCATGCCGGTTAAACCAGAGGTGAt                              >  1:4611789/1‑69 (MQ=255)                        ataaGCCTTCAATCAAATCAAGATTAAACAGCCACACCGCCATCATGCCGGTTAAACCAGAGGTGATAAg                           <  1:2170105/70‑1 (MQ=255)                                        atcaaGATTAAACAGCCACACCGCCCTCATGCCGGTTAAACCAGAGGCGATAAGCACGCCCAGCGTCGc            >  1:3584792/1‑69 (MQ=255)                                        atcaaGATTAAACAGCCACACCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGc            >  1:682049/1‑69 (MQ=255)                                        atcaaGATTAAACAGCCACACCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGc            >  1:1223227/1‑69 (MQ=255)                                        atcaaGATTAAACAGCCACACCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGc            >  1:4611917/1‑69 (MQ=255)                                        atcaaGATTAAACAGCCACACCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGc            >  1:4606313/1‑69 (MQ=255)                                        atcaaGATTAAACAGCCACACCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGc            >  1:3863728/1‑69 (MQ=255)                                        atcaaGATTAAACAGCCACACCGCCATCATCCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGc            >  1:1624690/1‑69 (MQ=255)                                             gATTAAACAGCCACACCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGAc     <  1:4573513/71‑1 (MQ=255)                                             gATTAAACAGCCACACCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGAc     <  1:4393060/71‑1 (MQ=255)                                             gATTAAACAGCCACACCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGAc     <  1:2442257/71‑1 (MQ=255)                                             gATTAAACAGCCACACCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGAc     <  1:647793/71‑1 (MQ=255)                                             gATTAAACAGCCACACCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGAc     <  1:1441867/71‑1 (MQ=255)                                                tAAACAGCCACACCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGt  >  1:4684307/1‑71 (MQ=255)                                                tAAACAGCCACACCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGt  >  1:2968970/1‑71 (MQ=255)                                                tAAACAGCCACACCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGt  >  1:1424381/1‑71 (MQ=255)                                                 aaaCAGCCACACCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGt  <  1:22026/70‑1 (MQ=255)                                                 aaaCAGCCACACCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGt  <  1:1913228/70‑1 (MQ=255)                                                 aaaCAGCCACACCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGt  <  1:771941/70‑1 (MQ=255)                                                           |                                                            AGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGT  >  minE/793893‑794010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑