Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,069,493	G→A	M130I (ATG→ATA)	cfa →	cyclopropane fatty acyl phospholipid synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,069,493	0	G	A	100.0%	312.7 / NA	96	M130I (ATG→ATA)	cfa	cyclopropane fatty acyl phospholipid synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (54/42);  total (54/42)

GTAAAAAACGTGCCTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATAATCTGGAATCTGC  >  minE/1069426‑1069555                                                                    |                                                               gTAAAAAACGTGCCTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATACtt                                                             <  1:4075541/71‑1 (MQ=255) gTAAAAAACGTGCCTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATACtt                                                             <  1:4465871/71‑1 (MQ=255) gTAAAAAACGTGCCTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATACtt                                                             <  1:1827810/71‑1 (MQ=255)  tAAAAAACGTGCCTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATACtt                                                             <  1:2934517/70‑1 (MQ=255)                ggATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATc                                                         >  1:1730707/1‑60 (MQ=255)                ggATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCATGCaa                                              >  1:1950685/1‑71 (MQ=255)                 gATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCATGCAat                                             <  1:3491529/71‑1 (MQ=255)                 gATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCATGCAat                                             <  1:4149180/71‑1 (MQ=255)                 gATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCATGCAat                                             <  1:446635/71‑1 (MQ=255)                 gATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCATGCAat                                             <  1:4857656/71‑1 (MQ=255)                        ggCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCATGCAATATTCCTg                                      >  1:3811278/1‑71 (MQ=255)                        ggCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCATGCAATATTCCTg                                      >  1:2688624/1‑71 (MQ=255)                        ggCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCATGCAATATTCCTg                                      >  1:167416/1‑71 (MQ=255)                        ggCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCATGCAATATTCCTg                                      >  1:3982867/1‑71 (MQ=255)                        ggCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCATGCAATATTCCTg                                      >  1:3557193/1‑71 (MQ=255)                        ggCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCATGCAATATTCCTg                                      >  1:4493742/1‑71 (MQ=255)                        ggCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCATGCAATATTCCTg                                      >  1:2904052/1‑71 (MQ=255)                            aaGAGCATTATGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCATGCAATATTCCTGCGCt                                  <  1:3371432/71‑1 (MQ=255)                            aaGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCATGCAATATTCCTGCGCt                                  <  1:1562539/71‑1 (MQ=255)                            aaGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCATGCAATATTCCTGCGCt                                  <  1:725924/71‑1 (MQ=255)                            aaGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCATGCAATATTCCTGCGCt                                  <  1:234044/71‑1 (MQ=255)                            aaGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCATGCAATATTCCTGCGCt                                  <  1:2184305/71‑1 (MQ=255)                            aaGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCATGCAATATTCCTGCGCt                                  <  1:4240195/71‑1 (MQ=255)                            aaGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCATGCAATATTCCTGCGCt                                  <  1:1152225/71‑1 (MQ=255)                            aaGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCATGCAATATTCCTGCGCt                                  <  1:5894/71‑1 (MQ=255)                            aaGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCATGCAATATTCCTGCGCt                                  <  1:3603371/71‑1 (MQ=255)                            aaGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCATGCAATATTCCTGCGCt                                  <  1:1749032/71‑1 (MQ=255)                            aaGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCATGCAATATTCCTGCGCt                                  <  1:334289/71‑1 (MQ=255)                            aaGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCATGCAATATTCCTGCGCt                                  <  1:4866893/71‑1 (MQ=255)                              gagCATTACGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCATGCAATATTCCTGCGCtt                                 >  1:2247836/1‑70 (MQ=255)                              gagCATTACGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCATGCAATATTCCTGCGCTTa                                >  1:509614/1‑71 (MQ=255)                                 cATTACGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCa                                                   >  1:2916443/1‑49 (MQ=255)                                 cATTACGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCa                                                   >  1:2895696/1‑49 (MQ=255)                                 cATTACGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCa                                                   >  1:2936798/1‑49 (MQ=255)                                 cATTACGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCa                                                   >  1:2768216/1‑49 (MQ=255)                                 cATTACGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCa                                                   >  1:1000367/1‑49 (MQ=255)                                 cATTACGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCa                                                   >  1:2099895/1‑49 (MQ=255)                                 cATTACGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCa                                                   >  1:402434/1‑49 (MQ=255)                                 cATTACGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCa                                                   >  1:1751566/1‑49 (MQ=255)                                 cATTACGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCa                                                   >  1:1022041/1‑49 (MQ=255)                                 cATTACGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCa                                                   >  1:1324668/1‑49 (MQ=255)                                 cATTACGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCa                                                   >  1:4900366/1‑49 (MQ=255)                                 cATTACGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCa                                                   >  1:2619529/1‑49 (MQ=255)                                 cATTACGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCa                                                   >  1:1841935/1‑49 (MQ=255)                                 cATTACGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCa                                                   >  1:2049278/1‑49 (MQ=255)                                 cATTACGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCa                                                   >  1:2115339/1‑49 (MQ=255)                                 cATTACGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCa                                                   >  1:4087126/1‑49 (MQ=255)                                   ttACGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCATg                                                 <  1:3638709/49‑1 (MQ=255)                                   ttACGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCATg                                                 <  1:1104109/49‑1 (MQ=255)                                     aCGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCa                                                   >  1:4714832/1‑45 (MQ=255)                                     aCGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCa                                                   >  1:2877096/1‑45 (MQ=255)                                     aCGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCa                                                   >  1:148584/1‑45 (MQ=255)                                     aCGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCa                                                   >  1:1475903/1‑45 (MQ=255)                                     aCGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCa                                                   >  1:1356679/1‑45 (MQ=255)                                     aCGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCATGCa                                               >  1:4762087/1‑49 (MQ=255)                                     aCGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCATGCa                                               >  1:2955081/1‑49 (MQ=255)                                     aCGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCATGCa                                               >  1:204162/1‑49 (MQ=255)                                     aCGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCATGCa                                               >  1:3378195/1‑49 (MQ=255)                                     aCGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCATGCa                                               >  1:2401453/1‑49 (MQ=255)                                     aCGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCATGCa                                               >  1:2433152/1‑49 (MQ=255)                                     aCGATTTGGGTAATGACTTGTTCAGCCGCATACTTGATCCCTTCATGCAata                                            >  1:239852/1‑52 (MQ=255)                                               tAATGACTTGTTCAGCCGCATACTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATa               <  1:1152399/71‑1 (MQ=255)                                               tAATGACTTGTTCAGCCGCATACTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATa               <  1:386412/71‑1 (MQ=255)                                               tAATGACTTGTTCAGCCGCATACTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATa               <  1:3606907/71‑1 (MQ=255)                                                aaTGACTTGTTCAGCCGCATACTTGATCCCTTCATGCAATATTCCTGCGCTTACt                              >  1:3723223/1‑55 (MQ=255)                                                    aCTTGTTCAGCCGCATACTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATAATCTg          >  1:1038200/1‑71 (MQ=255)                                                    aCTTGTTCAGCCGCATACTTGATACCTTCATGCAATATTCATGCGCTTACTGGAAAg                        >  1:1686165/1‑57 (MQ=255)                                                      ttGTTCAGCCGCATACTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATAATCTgg         >  1:3802441/1‑70 (MQ=255)                                                      ttGTTCAGCCGCATACTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATAATCTgg         >  1:1877109/1‑70 (MQ=255)                                                      ttGTTCAGCCGCATACTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATAATCTgg         >  1:3350758/1‑70 (MQ=255)                                                      ttGTTCAGCCGCATACTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATAATCTgg         >  1:1368532/1‑70 (MQ=255)                                                           cAGCCGCATACTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATAATCTGGAATCTg   >  1:904076/1‑71 (MQ=255)                                                           cAGCCGCATACTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATAATCTGGAATCTg   >  1:4570582/1‑71 (MQ=255)                                                           cAGCCGCATACTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATAATCTGGAATCTg   >  1:1976269/1‑71 (MQ=255)                                                           cAGCCGCATACTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATAATCTGGAATCTg   >  1:582583/1‑71 (MQ=255)                                                           cAGCCGCATACTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATAATCTGGAATCTg   >  1:721781/1‑71 (MQ=255)                                                           cAGCCGCATACTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATAATCTGGAATCTg   >  1:3016378/1‑71 (MQ=255)                                                           cAGCCGCATACTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATAATCT‑GAATCTGc  >  1:4240229/1‑71 (MQ=255)                                                           cAGCCGCATACTTGATCCCTTCATGCAATATTCCTGCGCTTACAGGAAAGATGCCGATAATCTGGAATCTg   >  1:603387/1‑71 (MQ=255)                                                                gCATACTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGc                    <  1:1648080/49‑1 (MQ=255)                                                                gCATACTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGc                    <  1:4323873/49‑1 (MQ=255)                                                                gCATACTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGc                    <  1:2001507/49‑1 (MQ=255)                                                                gCATACTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGc                    <  1:4352232/49‑1 (MQ=255)                                                                gCATACTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGc                    <  1:4299494/49‑1 (MQ=255)                                                                gCATACTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGc                    <  1:2114172/49‑1 (MQ=255)                                                                gCATACTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGc                    <  1:4056733/49‑1 (MQ=255)                                                                gCATACTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGc                    <  1:2652796/49‑1 (MQ=255)                                                                gCATACTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGc                    <  1:2893767/49‑1 (MQ=255)                                                                gCATACTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGc                    <  1:3052854/49‑1 (MQ=255)                                                                gCATACTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGc                    <  1:3545551/49‑1 (MQ=255)                                                                 cATACTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGc                    <  1:3478253/48‑1 (MQ=255)                                                                 cATACTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATAATCTgg         <  1:2076511/59‑1 (MQ=255)                                                                 cATACTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATAATCTgg         <  1:4384028/59‑1 (MQ=255)                                                                 cATACTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATAATCTgg         <  1:48577/59‑1 (MQ=255)                                                                 cATACTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATAATCTgg         <  1:1608796/59‑1 (MQ=255)                                                                 cATACTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATAATCTgg         <  1:3926602/59‑1 (MQ=255)                                                                    |                                                               GTAAAAAACGTGCCTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATAATCTGGAATCTGC  >  minE/1069426‑1069555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑