BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	161,367	A→G	N27S (AAC→AGC)	cdaR →	DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	161,367	0	A	G	100.0%	475.1 / NA	126	N27S (AAC→AGC)	cdaR	DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (97/29); total (97/29)

Only 100 of 126 total aligned reads displayed.

CTTGATACCAAAATGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGTATTGGTGAATTGCACG  >  minE/161303‑161432

cTTGATACCAAAATGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAGCGTAAt                                                             >  1:2867318/1‑71 (MQ=255)

cTTGATACCAAAATGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAGCGTAAt                                                             >  1:4600511/1‑71 (MQ=255)

cTTGATACCAAAATGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAGCGTAAt                                                             >  1:3978475/1‑71 (MQ=255)

cTTGATACCAAAATGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAGCGTAAt                                                             >  1:4512588/1‑71 (MQ=255)

cTTGATACCAAAATGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAGCGTAAt                                                             >  1:446774/1‑71 (MQ=255)

cTTGATACCAAAATGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAGCGTAAt                                                             >  1:2772769/1‑71 (MQ=255)

cTTGATACCAAAATGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAGCGTAAt                                                             >  1:2065554/1‑71 (MQ=255)

cTTGATACCAAAATGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAGCGTAAt                                                             >  1:2375725/1‑71 (MQ=255)

 ttGATACCAAAATGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAGCGTaa                                                              >  1:4207978/1‑69 (MQ=255)

 ttGATACCAAAATGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAGCGTaa                                                              >  1:3581956/1‑69 (MQ=255)

 ttGATACCAAAATGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAGCGTaa                                                              >  1:1018553/1‑69 (MQ=255)

 ttGATACCAAAATGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAGCGTaa                                                              >  1:2490833/1‑69 (MQ=255)

 ttGATACCAAAATGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAGCGTaa                                                              >  1:1874743/1‑69 (MQ=255)

 ttGATACCAAAATGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAGCGTaa                                                              >  1:2130232/1‑69 (MQ=255)

 ttGATACCAAAATGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAGCGTaa                                                              >  1:3576491/1‑69 (MQ=255)

 ttGATACCAAAATGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAGCGTaa                                                              >  1:4305992/1‑69 (MQ=255)

 ttGATACCAAAATGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAGCGTaa                                                              >  1:1716020/1‑69 (MQ=255)

 ttGATACCAAAATGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAGCGTaa                                                              >  1:1570508/1‑69 (MQ=255)

 ttGATACCAAAATGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAGCGTaa                                                              >  1:1570166/1‑69 (MQ=255)

 ttGATACCAAAATGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAGCGTaa                                                              >  1:3307842/1‑69 (MQ=255)

 ttGATACCAAAATGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAGCGTaa                                                              >  1:283867/1‑69 (MQ=255)

 ttGATACCAAAATGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAGCGTaa                                                              >  1:1204491/1‑69 (MQ=255)

 ttGATACCAAAATGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAGCGTaa                                                              >  1:340647/1‑69 (MQ=255)

 ttGATACCAAAATGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAGCGTAATg                                                            >  1:4276918/1‑71 (MQ=255)

 ttGATACCAAAATGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAGCGTAATg                                                            >  1:4155838/1‑71 (MQ=255)

 ttGATACCAAAATGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAGCGTAATg                                                            >  1:1811128/1‑71 (MQ=255)

         aaaaTGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAGCGTAATGGATGccc                                                     >  1:3785892/1‑70 (MQ=255)

         aaaaTGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAGCGTAATGGATGccc                                                     >  1:751338/1‑70 (MQ=255)

         aaaaTGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAGCGTAATGGATGccc                                                     >  1:3844044/1‑70 (MQ=255)

         aaaaTGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAGCGTAATGGATGccc                                                     >  1:2496150/1‑70 (MQ=255)

         aaaaTGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAGCGTAATGGATGccc                                                     >  1:4610941/1‑70 (MQ=255)

         aaaaTGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAGCGTAATGGATGcc                                                      >  1:3936814/1‑69 (MQ=255)

         aaaaTGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAGCGTAATGGATGCCCg                                                    >  1:29987/1‑71 (MQ=255)

         aaaaTGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAGCGTAATGGATGCCCg                                                    >  1:3292281/1‑71 (MQ=255)

         aaaaTGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAGCGTAATGGATGCCCg                                                    >  1:3486586/1‑71 (MQ=255)

         aaaaTGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAGCGTAATGGATGCCCg                                                    >  1:775695/1‑71 (MQ=255)

         aaaaTGGCGCAGGATATCGTGGCACGTACCATGCGCATCATAGATACCAATATCAGCGTAATGGATGCCCg                                                    >  1:1899441/1‑71 (MQ=255)

         aaaaAGGCGCAGGATATCGTGGCACGTACAATGCGCATCATCGATACCAATATCAGCGTAATGGATGCCCg                                                    >  1:2585212/1‑71 (MQ=255)

                                  gTACCATGCGCATCATCGATACCAATATCAGCGTAATGGATGCCTGTGGGCGAATTATCGGCAGCGGCGAt                           <  1:1331976/71‑1 (MQ=255)

                                  gTACCATGCGCATCATCGATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGAt                           <  1:2458203/71‑1 (MQ=255)

                                  gTACCATGCGCATCATCGATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGAt                           <  1:85308/71‑1 (MQ=255)

                                  gTACCATGCGCATCATCGATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGAt                           <  1:1213372/71‑1 (MQ=255)

                                  gTACCATGCGCATCATCGATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGAt                           <  1:1385744/71‑1 (MQ=255)

                                  gTACCATGCGCATCATCGATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGAt                           <  1:1402442/71‑1 (MQ=255)

                                  gTACCATGCGCATCATCGATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGAt                           <  1:3480854/71‑1 (MQ=255)

                                    aCCATGCGCATCATCGATACCAATATCAGCGTAATGGATGcc                                                      >  1:2030925/1‑42 (MQ=255)

                                    aCCATGCGCATCATCGATACCAATATCAGCGTAATGGATGCCCGTGGGCGAAt                                           >  1:4062810/1‑53 (MQ=255)

                                    aCCATGCGCATCATCGATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATc                          >  1:4203583/1‑70 (MQ=255)

                                    aCCATGCGCATCATCGATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATc                          >  1:708776/1‑70 (MQ=255)

                                    aCCATGCGCATCATCGATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCg                         >  1:3847424/1‑71 (MQ=255)

                                    aCCATGCGCATCATCGATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCg                         >  1:3913516/1‑71 (MQ=255)

                                    aCCATGCGCATCATCGATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCg                         >  1:3290224/1‑71 (MQ=255)

                                    aCCATGCGCATCATCGATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCg                         >  1:2921378/1‑71 (MQ=255)

                                    aCCATGCGCATCATCGATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCg                         >  1:2363778/1‑71 (MQ=255)

                                    aCCATGCGCATCATCGATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCg                         >  1:4378942/1‑71 (MQ=255)

                                    aCCATGCGCATCATCGATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCg                         >  1:1929946/1‑71 (MQ=255)

                                    aCCATGCGCATCATCGATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCg                         >  1:1754737/1‑71 (MQ=255)

                                    aCCATGCGCATCATCGATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCg                         >  1:1371158/1‑71 (MQ=255)

                                    aCCATGCGCATCATCGATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCg                         >  1:826211/1‑71 (MQ=255)

                                       aTGCGCATCATCGATACCAATATCAGCGTAATGGAt                                                         >  1:171023/1‑36 (MQ=255)

                                       aTGCGCATCATCGATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAg                                  >  1:3083543/1‑59 (MQ=255)

                                       aTGCGCATCATCGATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCg                         >  1:2689101/1‑68 (MQ=255)

                                       aTGCGCATCATCGATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGt                        >  1:4008234/1‑69 (MQ=255)

                                       aTGCGCATCATCGATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGt                        >  1:3990816/1‑69 (MQ=255)

                                       aTGCGCATCATCGATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGt                        >  1:3962999/1‑69 (MQ=255)

                                       aTGCGCATCATCGATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGt                        >  1:4581368/1‑69 (MQ=255)

                                       aTGCGCATCATCGATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGt                        >  1:109440/1‑69 (MQ=255)

                                       aTGCGCATCATCGATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGt                        >  1:3786557/1‑69 (MQ=255)

                                       aTGCGCATCATCGATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGt                        >  1:1160278/1‑69 (MQ=255)

                                       aTGCGCATCATCGATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGt                        >  1:315682/1‑69 (MQ=255)

                                       aTGCGCATCATCGATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGt                        >  1:2647418/1‑69 (MQ=255)

                                       aTGCGCATCATCGATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGt                        >  1:2333521/1‑69 (MQ=255)

                                       aTGCGCATCATCGATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGt                        >  1:1822295/1‑69 (MQ=255)

                                       aTGCGCATCATCGATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGt                        >  1:156312/1‑69 (MQ=255)

                                        tGCGCATCATCGATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAg                     <  1:3934379/71‑1 (MQ=255)

                                        tGCGCATCATCGATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAg                     <  1:729565/71‑1 (MQ=255)

                                        tGCGCATCATCGATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAg                     <  1:4853535/71‑1 (MQ=255)

                                        tGCGCATCATCGATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAg                     <  1:4716122/71‑1 (MQ=255)

                                           gcATCATCGATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGt                  <  1:4834201/71‑1 (MQ=255)

                                            catcatCGATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGt                  <  1:4733108/70‑1 (MQ=255)

                                              tcatcGATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGTAtt               >  1:1121035/1‑71 (MQ=255)

                                              tcatcGATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGTAtt               >  1:826426/1‑71 (MQ=255)

                                              tcatcGATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGTAtt               >  1:1257791/1‑71 (MQ=255)

                                              tcatcGATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGTAtt               >  1:3227658/1‑71 (MQ=255)

                                              tcatcGATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGTAtt               >  1:454876/1‑71 (MQ=255)

                                              tcatcGATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGTAt                >  1:334995/1‑70 (MQ=255)

                                              tcatcGATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGTAt                >  1:1185416/1‑70 (MQ=255)

                                              tcatcGATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGTAt                >  1:2154741/1‑70 (MQ=255)

                                                   gATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGTATTGGTg           <  1:2463047/70‑1 (MQ=255)

                                                   gATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGTATTGGTg           <  1:361335/70‑1 (MQ=255)

                                                   gATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGTATTGGTg           <  1:3136743/70‑1 (MQ=255)

                                                   gATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGTATTGGTg           <  1:4854237/70‑1 (MQ=255)

                                                   gATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGTATTGGTg           <  1:2360427/70‑1 (MQ=255)

                                                   gATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGTATTGGTg           <  1:4322911/70‑1 (MQ=255)

                                                   gATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGTATTGGTg           <  1:4701063/70‑1 (MQ=255)

                                                   gATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGTATTGGTg           <  1:1810504/70‑1 (MQ=255)

                                                   gATACCAATATCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGTATTGGTg           <  1:1667822/70‑1 (MQ=255)

                                                             tCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGTATTGGTGAATTGCACg  >  1:1467518/1‑69 (MQ=255)

                                                             tCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGTATTGGTGAATTGCACg  >  1:3611147/1‑69 (MQ=255)

                                                             tCAGCGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGTATTGGTGAATTGCACg  >  1:1672569/1‑69 (MQ=255)

CTTGATACCAAAATGGCGCAGGATATCGTGGCACGTACCATGCGCATCATCGATACCAATATCAACGTAATGGATGCCCGTGGGCGAATTATCGGCAGCGGCGATCGTGAGCGTATTGGTGAATTGCACG  >  minE/161303‑161432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`