Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,109,347	C→T	L88F (CTC→TTC)	ydiS →	predicted oxidoreductase, FAD/NAD(P)‑binding domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,109,347	0	C	T	100.0%	153.4 / NA	47	L88F (CTC→TTC)	ydiS	predicted oxidoreductase, FAD/NAD(P)‑binding domain
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/13);  total (34/13)

AGCGCCGGTAGAACGCAAGGTCACACGCGAGAAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCCTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAACACGCATCTTATACCGTATTGCGTAATCGTCT  >  minE/1109280‑1109414                                                                    |                                                                    aGCGCCGGTAGAACGCAAGGTCACACGCGAGAAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCTTc                                                                   >  1:1310767/1‑70 (MQ=255) aGCGCCGGTAGAACGCAAGGTCACACGCGAGAAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCTTc                                                                   >  1:3183529/1‑70 (MQ=255)       ggTAGAACGCAAGGTCACACGCGAGAAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCTTCGATTTTc                                                            <  1:1805864/71‑1 (MQ=255)        gTAGAACGCAAGGTCACACGCGAGAAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCTTCGATTTTCa                                                           >  1:466994/1‑71 (MQ=255)              cGCAAGGTCACACGCGAGAAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCTTCGATTTTCACcgc                                                       >  1:1512581/1‑69 (MQ=255)              cGCAAGGTCACACGCGAGAAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCTTCGATTTTCACcgc                                                       >  1:2258067/1‑69 (MQ=255)              cGCAAGGTCACACGCGAGAAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCTTCGATTTTCACcgc                                                       >  1:2454776/1‑69 (MQ=255)              cGCAAGGTCACACGCGAGAAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCTTCGATTTTCACcgc                                                       >  1:2473856/1‑69 (MQ=255)              cGCAAGGTCACACGCGAGAAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCTTCGATTTTCACcgc                                                       >  1:4220655/1‑69 (MQ=255)              cGCAAGGTCACACGCGAGAAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCTTCGATTTTCACcgc                                                       >  1:3040127/1‑69 (MQ=255)              cGCAAGGTCACACGCGAGAAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCTTCGATTTTCACcgc                                                       >  1:4915690/1‑69 (MQ=255)              cGCAAGGTCACACGCGAGAAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCTTCGATTTTCACcgc                                                       >  1:4442826/1‑69 (MQ=255)                cAAGGTCACACGCGAGAAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCTTCGATTTTCACCGCGag                                                    >  1:3313967/1‑70 (MQ=255)                cAAGGTCACACGCGAGAAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCTTCGATTTTCACCGCGag                                                    >  1:4206060/1‑70 (MQ=255)                cAAGGTCACACGCGAGAAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCTTCGATTTTCACCGCGag                                                    >  1:230542/1‑70 (MQ=255)                cAAGGTCACACGCGAGAAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCTTCGATTTTCACCGCGag                                                    >  1:4789113/1‑70 (MQ=255)                            cgAGAAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCTTCGATTTTCACCGCGAGCAGCCAGATGTTc                                       >  1:1709907/1‑71 (MQ=255)                               gaAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCTTCGATTTTCACCGCGAGCAGCCAGATGTTCCGc                                    <  1:3580762/71‑1 (MQ=255)                               gaAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCTTCGATTTTCACCGCGAGCAGCCAGATGTTCCGc                                    <  1:1396272/71‑1 (MQ=255)                                 aaaTCTCCTTCTTAACCGAAGAAAGCGCCGTTACCTTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCaa                                  <  1:4705835/71‑1 (MQ=255)                                 aaaTCTCCTTCTTAACCGAAGAAAGCGCCGTTACCTTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCaa                                  <  1:2519895/71‑1 (MQ=255)                                   aTCTCCTTCTTAACCGAAGAAAGCGCCGTTACCTTCGAtt                                                               >  1:179013/1‑40 (MQ=255)                                   aTCTCCTTCTTAACCGAAGAAAGCGCCGTTACCTTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAaca                                >  1:4860606/1‑71 (MQ=255)                                   aTCTCCTTCTTAACCGAAGAAAGCGCCGTTACCTTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAaca                                >  1:4338210/1‑71 (MQ=255)                                   aTCTCCTTCTTAACCGAAGAAAGCGCCGTTACCTTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAaca                                >  1:510029/1‑71 (MQ=255)                                   aTCTCCTTCTTAACCGAAGAAAGCGCCGTTACCTTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAaca                                >  1:789233/1‑71 (MQ=255)                                   aTCTCCTTCTTAACCGAAGAAAGCGCCGTTACCTTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAaca                                >  1:946784/1‑71 (MQ=255)                                   aTCTCCTTCTTAACCGAAGAAAGCGCCGTTACCTTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAaca                                >  1:3169809/1‑71 (MQ=255)                                   aTCTCCTTCTTAACCGAAGAAAGCGCCGTTACCTTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAaca                                >  1:3099213/1‑71 (MQ=255)                                   aTCTCCTTCTTAACCGAAGAAAGCGCCGTTACCTTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAaca                                >  1:2571301/1‑71 (MQ=255)                                   aTCTCCTTCTTAACCGAAGAAAGCGCCGTTACCTTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAaca                                >  1:296954/1‑71 (MQ=255)                                     ctcCTTCTTAACCGAAGAAAGCGCCGTTACCTTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAACACg                              >  1:2985476/1‑71 (MQ=255)                                     ctcCTTCTTAACCGAAGAAAGCGCCGTTACCTTCGATTTTCACCGCGAGAAGCCAGATGTTCCGCAACACg                              >  1:412992/1‑71 (MQ=255)                                         ttcttAACCGAATAAAGCGCCGTTACCTTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAACACGCATc                          >  1:281925/1‑71 (MQ=255)                                         ttcttAACCGAAGAAAGCGCCGTTACCTTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAACACGCATc                          >  1:1060855/1‑71 (MQ=255)                                         ttcttAACCGAAGAAAGCGCCGTTACCTTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAACACGCATc                          >  1:37751/1‑71 (MQ=255)                                         ttcttAACCGAAGAAAGCGCCGTTACCTTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAACACGCATc                          >  1:4521788/1‑71 (MQ=255)                                         ttcttAACCGAAGAAAGCGCCGTTACCTTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAACACGCATc                          >  1:3558426/1‑71 (MQ=255)                                           cttAACCGAAGAAAGCGCCGTTACCTTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAACACGCATCt                         <  1:4545292/70‑1 (MQ=255)                                           cttAACCGAAGAAAGCGCCGTTACCTTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAACACGCATCt                         <  1:1981416/70‑1 (MQ=255)                                           cttAACCGAAGAAAGCGCCGTTACCTTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAACACGCATCt                         <  1:2533821/70‑1 (MQ=255)                                           cttAACCGAAGAAAGCGCCGTTACCTTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAACACGCATCt                         <  1:2711019/70‑1 (MQ=255)                                           cttAACCGAAGAAAGCGCCGTTACCTTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAACACGCATCt                         <  1:2914728/70‑1 (MQ=255)                                           cttAACCGAAGAAAGCGCCGTTACCTTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAACACGCATCt                         <  1:462865/70‑1 (MQ=255)                                           cttAACCGAAGAAAGCGCCGTTACCTTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAACACGCATCt                         <  1:2973842/70‑1 (MQ=255)                                           cttAACCGAAGAAAGCGCCGTTACCTTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAACACCCATCt                         <  1:4475266/70‑1 (MQ=255)                                                                 aCCTTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAACACGCATCTTATACCGTATTGCGTAATCGTCt  >  1:3732472/1‑71 (MQ=255)                                                                    |                                                                    AGCGCCGGTAGAACGCAAGGTCACACGCGAGAAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCCTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAACACGCATCTTATACCGTATTGCGTAATCGTCT  >  minE/1109280‑1109414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑