Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,358,189	G→T	S311R (AGC→AGA)	yehB ←	predicted outer membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,358,189	0	G	T	100.0%	196.4 / NA	61	S311R (AGC→AGA)	yehB	predicted outer membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (28/33);  total (28/33)

GCTGCATAAGGCACCAGATAGGTGGTTACCGAGCCGTCCGCCTCTTTCACGCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAATCGCGAACGGGCCAGGAGG  >  minE/1358139‑1358254                                                   |                                                                  gCTGCATAAGGCACCAGATAGGTGGTTACCGAGCCGTCCGCCTCTTTCCCTCTGACATCAAGATCt                                                    >  1:1402444/1‑66 (MQ=255) gCTGCATAAGGCACCAGATAGGTGGTTACCGAGCCGTCCGCCTCTTTCACTCTGACATCa                                                          >  1:2413082/1‑60 (MQ=255) gCTGCATAAGGCACCAGATAGGTGGTTACCGAGCCGTCCGCCTCTTTCACTCTGACATCAAGATCTGCTc                                                >  1:1507248/1‑70 (MQ=255)           gCACCAGATAGGTGGTTACCGAGCCGTCCGCCTCTTTCACTCTGACATCAAGATCTGCTCCACCACCGGc                                      >  1:4009442/1‑70 (MQ=255)           gCACCAGATAGGTGGTTACCGAGCCGTCCGCCTCTTTCACTCTGACATCAAGATCTGCTCCACCACCGGc                                      >  1:2306092/1‑70 (MQ=255)           gCACCAGATAGGTGGTTACCGAGCCGTCCGCCTCTTTCACTCTGACATCAAGATCTGCTCCACCACCGGc                                      >  1:1915584/1‑70 (MQ=255)                 gATAGGTGGTTACCGAGCCGTCCGCCTCTTTCACTCTGACATCAAGATCTGCTCCACCACCGGCCAACTGc                               <  1:2757203/71‑1 (MQ=255)                   tAGGTGGTTACCGAGCCGTCCGCCTCTTTCACTCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCa                              >  1:2229830/1‑70 (MQ=255)                   tAGGTGGTTACCGAGCCGTCCGCCTCTTTCACTCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCa                              >  1:2910387/1‑70 (MQ=255)                   tAGGTGGTTACCGAGCCGTCCGCCTCTTTCACTCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCa                              >  1:4509201/1‑70 (MQ=255)                   tAGGTGGTTACCGAGCCGTCCGCCTCTTTCACTCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCa                              >  1:1756796/1‑70 (MQ=255)                   tAGGTGGTTACCGAGCCGTCCGCCTCTTTAACTCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCa                              >  1:1753005/1‑70 (MQ=255)                    aGGTGGTTACCGAGCCGTCCGCCTCTTTCCCTCTGa                                                               >  1:2076603/1‑36 (MQ=255)                    aGGTGGTTACCGAGCCGTCCGCCTCTTTCACTCTGACATCAAg                                                        >  1:4175855/1‑43 (MQ=255)                    aGGTGGTTACCGAGCCGTCCGCCTCTTTCACTCTGACATCAAGa                                                       >  1:2324970/1‑44 (MQ=255)                    aGGTGGTTACCGAGCCGTCCGCCTCTTTCACTCTGACATCAAGa                                                       >  1:3680395/1‑44 (MQ=255)                    aGGTGGTTACCGAGCCGTCCGCCTCTTTCACTCTGACATCAAGAt                                                      >  1:360455/1‑45 (MQ=255)                    aGGTGGTTACCGAGCCGTCCGCCTCTTTCACTCTGACATCAAGAt                                                      >  1:3293341/1‑45 (MQ=255)                    aGGTGGTTACCGAGCCGTCCGCCTCTTTCACTCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCaaa                            >  1:1867147/1‑71 (MQ=255)                    aGGTGGTTACCGAGCCGTCCGCCTCTTTCACTCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCaaa                            >  1:4409765/1‑71 (MQ=255)                    aGGTGGTTACCGAGCCGTCCGCCTCTTTCACTCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCaa                             >  1:4589137/1‑70 (MQ=255)                       tggtTACCGAGCCGTCCGCCTCTTTCACTCTGACATCAAGATCTGCTCCACCACCTGCCAACTGCAAATCt                         >  1:2253735/1‑71 (MQ=255)                       tggtTACCGAGCCGTCCGCCTCTTTCACTCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATc                          >  1:4246063/1‑70 (MQ=255)                       tggtTACCGAGCCGTCCGCCTCTTTCACTCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCt                         <  1:1852475/71‑1 (MQ=255)                       tggtTACCGAGCCGTCCGCCTCTTTCACTCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCt                         <  1:2478117/71‑1 (MQ=255)                       tggtTACCGAGCCGTCCGCCTCTTTCACTCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCt                         <  1:4192847/71‑1 (MQ=255)                       tggtTACCGAGCCGTCCGCCTCTTTCACTCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCt                         <  1:3372641/71‑1 (MQ=255)                       tggtTACCGAGCCGTCCGCCTCTTTCACTCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCt                         >  1:3852272/1‑71 (MQ=255)                       tggtTACCGAGCCGTCCGCCTCTTTCACTCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCt                         <  1:2889024/71‑1 (MQ=255)                       tggtTACCGAGCCGTCCGCCTCTTTCACTCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCt                         >  1:100372/1‑71 (MQ=255)                         gtTACCGAGCCGTCCGCCTCTTTCACTCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTg                        >  1:2586200/1‑70 (MQ=255)                         gtTACCGAGCCGTCCGCCTCTTTCACTCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTg                        >  1:26290/1‑70 (MQ=255)                         gtTACCGAGCCGTCCGCCTCTTTCACTCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTg                        >  1:3481342/1‑70 (MQ=255)                            aCCGAGCCGTCCGCCTCTTTCACTCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGTaa                     <  1:1387426/70‑1 (MQ=255)                            aCCGAGCCGTCCGCCTCTTTCACTCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGTaa                     <  1:4594220/70‑1 (MQ=255)                            aCCGAGCCGTCCGCCTCTTTCACTCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGTaa                     <  1:3929194/70‑1 (MQ=255)                            aCCGAGCCGTCCGCCTCTTTCACTCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGTaa                     <  1:4271578/70‑1 (MQ=255)                                   cGTCCGCCTCTTTCACTCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAATCGCGAAc             <  1:1784558/71‑1 (MQ=255)                                   cGTCCGCCTCTTTCACTCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAATCGCGAAc             <  1:794220/71‑1 (MQ=255)                                   cGTCCGCCTCTTTCACTCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAATCGCGAAc             <  1:2196498/71‑1 (MQ=255)                                   agTCCGCCTCTTTCACTCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAATCGCGAAc             <  1:1639844/70‑1 (MQ=255)                                          ctctTTCACTCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAATCGCGAACGGGCCag      <  1:652948/71‑1 (MQ=255)                                          ctctTTCACTCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAATCGCGAACGGGCCag      <  1:3142942/71‑1 (MQ=255)                                          ctctTTCACTCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAATCGCGAACGGGCCag      <  1:3109793/71‑1 (MQ=255)                                          ctctTTCACTCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAATCGCGAACGGGCCag      <  1:3831565/71‑1 (MQ=255)                                          ctctTTCACTCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAATCGCGAACGGGCCag      <  1:4537946/71‑1 (MQ=255)                                          ctctTTCACTCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAATCGCGAACGGGCCag      <  1:2202057/71‑1 (MQ=255)                                          ctctTTCACTCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAATCGCGAACGGGCCag      <  1:4186622/71‑1 (MQ=255)                                          ctctTTCACTCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAATCGCGAACGGGCCag      <  1:1815270/71‑1 (MQ=255)                                             tttCACTCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAATcgcg                <  1:4190856/58‑1 (MQ=255)                                             tttCACTCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAATcgcg                <  1:1524860/58‑1 (MQ=255)                                             tttCACTCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAATcgcg                <  1:1980177/58‑1 (MQ=255)                                             tttCACTCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAATcgcg                <  1:2483594/58‑1 (MQ=255)                                             tttCACTCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAATcgcg                <  1:2718121/58‑1 (MQ=255)                                             tttCACTCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAATcgcg                <  1:2800417/58‑1 (MQ=255)                                             tttCACTCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAATcgcg                <  1:3071366/58‑1 (MQ=255)                                               tCACTCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAATCGCGaa              >  1:1556446/1‑58 (MQ=255)                                               tCACTCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAATCGCGAACGGGCCaggagg  <  1:929954/70‑1 (MQ=255)                                               tCACTCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAATCGCGAACGGGCCaggagg  <  1:3262642/70‑1 (MQ=255)                                               tCACTCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAATCGCGAACGGGCCaggagg  <  1:4318939/70‑1 (MQ=255)                                               tCACTCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAATCGCGAACGGGCCaggagg  <  1:315731/70‑1 (MQ=255)                                                   |                                                                  GCTGCATAAGGCACCAGATAGGTGGTTACCGAGCCGTCCGCCTCTTTCACGCTGACATCAAGATCTGCTCCACCACCGGCCAACTGCAAATCTGTAATCGCGAACGGGCCAGGAGG  >  minE/1358139‑1358254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑