BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,391,735	T→C	K370E (AAA→GAA)	fruA ←	fused fructose‑specific PTS enzyme IIB'BC components

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,391,735	0	T	C	100.0%	436.4 / NA	115	K370E (AAA→GAA)	fruA	fused fructose‑specific PTS enzyme IIB'BC components
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (57/58); total (57/58)

Only 100 of 115 total aligned reads displayed.

AACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGATC  >  minE/1391667‑1391793

aaCCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCAc                                                          <  1:1477635/71‑1 (MQ=255)

 aCCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCAc                                                          <  1:4662189/70‑1 (MQ=255)

 aCCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCAc                                                          <  1:4178033/70‑1 (MQ=255)

     cATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATc                                                     <  1:4840254/71‑1 (MQ=255)

     cATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATc                                                     <  1:1588285/71‑1 (MQ=255)

      aTTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATc                                                     <  1:1319917/70‑1 (MQ=255)

         cGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACGGATCAgg                                                  >  1:4799986/1‑70 (MQ=255)

         cGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAgg                                                  >  1:1725399/1‑70 (MQ=255)

         cGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAgg                                                  <  1:445999/70‑1 (MQ=255)

         cGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAgg                                                  >  1:3490317/1‑70 (MQ=255)

         cGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAgg                                                  >  1:3775068/1‑70 (MQ=255)

              tCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGatc                                            <  1:1814485/71‑1 (MQ=255)

              tCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGatc                                            <  1:3222088/71‑1 (MQ=255)

              tCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGatc                                            <  1:4797456/71‑1 (MQ=255)

                cccATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGatcat                                          >  1:4046485/1‑71 (MQ=255)

                cccATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGatcat                                          >  1:3259291/1‑71 (MQ=255)

                cccATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGatcat                                          >  1:96168/1‑71 (MQ=255)

                cccATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGatcat                                          >  1:1066350/1‑71 (MQ=255)

                cccATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGatcat                                          >  1:1698672/1‑71 (MQ=255)

                cccATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGatca                                           >  1:170167/1‑70 (MQ=255)

                cccATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGatca                                           >  1:1663023/1‑70 (MQ=255)

                accATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGatcat                                          >  1:1551957/2‑71 (MQ=255)

                   aTGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGATCATCGc                                       <  1:2746105/71‑1 (MQ=255)

                      gTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGATCATCGCCa                                     <  1:3946156/70‑1 (MQ=255)

                      gTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGATCATCGCCa                                     <  1:2637784/70‑1 (MQ=255)

                      gTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGATCATCGCCa                                     <  1:1556373/70‑1 (MQ=255)

                      gTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGATCATCGCCa                                     <  1:2412219/70‑1 (MQ=255)

                      gTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGATCATCGCCa                                     <  1:4111015/70‑1 (MQ=255)

                      gTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGATCATCGCCa                                     <  1:4762534/70‑1 (MQ=255)

                       tCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGATCATCGCCa                                     <  1:4105641/69‑1 (MQ=255)

                       tCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGATCATCGCCa                                     <  1:4636657/69‑1 (MQ=255)

                       tCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGATCATCGCCa                                     <  1:773444/69‑1 (MQ=255)

                         tGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAg                                               <  1:1874464/57‑1 (MQ=255)

                         tGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAg                                               <  1:3831898/57‑1 (MQ=255)

                         tGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAg                                               <  1:1377142/57‑1 (MQ=255)

                         tGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAg                                               <  1:127031/57‑1 (MQ=255)

                         tGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAg                                               <  1:494133/57‑1 (MQ=255)

                         tGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAg                                               <  1:306538/57‑1 (MQ=255)

                         tGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAg                                               <  1:2798215/57‑1 (MQ=255)

                         tGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAg                                               <  1:881638/57‑1 (MQ=255)

                         tGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAg                                               <  1:2433672/57‑1 (MQ=255)

                         tGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAg                                               <  1:2682780/57‑1 (MQ=255)

                         tGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAg                                               <  1:2478056/57‑1 (MQ=255)

                         tGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAg                                               <  1:2215807/57‑1 (MQ=255)

                         tGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAg                                               <  1:2662748/57‑1 (MQ=255)

                          gCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAg                                               <  1:2723944/56‑1 (MQ=255)

                          gCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAg                                               <  1:2703947/56‑1 (MQ=255)

                          gCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAg                                               <  1:3266689/56‑1 (MQ=255)

                           gaGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGatc                                            >  1:50747/2‑58 (MQ=255)

                           cAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGatc                                            >  1:4317850/1‑58 (MQ=255)

                           cAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGatc                                            >  1:3936877/1‑58 (MQ=255)

                           cAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGatc                                            >  1:3802964/1‑58 (MQ=255)

                           cAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGatc                                            >  1:4055491/1‑58 (MQ=255)

                           cAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGatc                                            >  1:4100911/1‑58 (MQ=255)

                           cAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGatc                                            >  1:3364225/1‑58 (MQ=255)

                           cAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGatc                                            >  1:2533473/1‑58 (MQ=255)

                           cAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGatc                                            >  1:1398759/1‑58 (MQ=255)

                           cAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGatc                                            >  1:214531/1‑58 (MQ=255)

                           cAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGatc                                            >  1:2057054/1‑58 (MQ=255)

                           cAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGatc                                            >  1:4818022/1‑58 (MQ=255)

                           cAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGatc                                            >  1:4823687/1‑58 (MQ=255)

                           cAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGat                                             >  1:950494/1‑57 (MQ=255)

                           cAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGat                                             >  1:4446049/1‑57 (MQ=255)

                           cAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGat                                             >  1:212085/1‑57 (MQ=255)

                           cAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGat                                             >  1:2374617/1‑57 (MQ=255)

                           cAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGat                                             >  1:1208864/1‑57 (MQ=255)

                           cAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGat                                             >  1:2940576/1‑57 (MQ=255)

                           cAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGat                                             >  1:2946188/1‑57 (MQ=255)

                           cAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGat                                             >  1:2446173/1‑57 (MQ=255)

                           cAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGat                                             >  1:3150287/1‑57 (MQ=255)

                           cAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGat                                             >  1:182942/1‑57 (MQ=255)

                           cAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGATCATCGCCAgacc                                 <  1:4830300/69‑1 (MQ=255)

                           cAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGATCATCGCCAgacc                                 <  1:1995508/69‑1 (MQ=255)

                           cAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGATCATCGCCAgacc                                 <  1:3104402/69‑1 (MQ=255)

                           cAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGATCATCGCCAgacc                                 <  1:4326510/69‑1 (MQ=255)

                           cAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGATCATCGCCAgacc                                 <  1:3409110/69‑1 (MQ=255)

                           cAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGATCATCGCCAgacc                                 <  1:2343122/69‑1 (MQ=255)

                           cAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGATCATCGCCAgacc                                 <  1:3768168/69‑1 (MQ=255)

                                    aGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGATCATCGCCAGACCGACCACCAGAc                      >  1:3308919/1‑71 (MQ=255)

                                    aGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGATCATCGCCAGACCGACCACCAGAc                      >  1:2885362/1‑71 (MQ=255)

                                      tCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGATCATCGc                                       <  1:71820/52‑1 (MQ=255)

                                       cAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTg                    >  1:1936241/1‑70 (MQ=255)

                                              tCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAg            >  1:817794/1‑71 (MQ=255)

                                              tCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAg            >  1:3870401/1‑71 (MQ=255)

                                               cGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGc           >  1:832037/1‑71 (MQ=255)

                                               cGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGc           >  1:888850/1‑71 (MQ=255)

                                               cGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGc           >  1:1929475/1‑71 (MQ=255)

                                               cGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGc           >  1:2738630/1‑71 (MQ=255)

                                               cGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGc           >  1:1160921/1‑71 (MQ=255)

                                               cGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGc           >  1:2402199/1‑71 (MQ=255)

                                               cGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGc           >  1:323067/1‑71 (MQ=255)

                                               cGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGc           >  1:3178114/1‑71 (MQ=255)

                                               cGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGc           >  1:312361/1‑71 (MQ=255)

                                               cGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGc           >  1:2733109/1‑71 (MQ=255)

                                                 agaATGCCAGCAACTGGTTCACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCgg         <  1:4930559/71‑1 (MQ=255)

                                                 agaATGCCAGCAACTGGTTCACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCgg         <  1:4762526/71‑1 (MQ=255)

                                                 agaATGCCAGCAACTGGTTCACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCgg         <  1:1539773/71‑1 (MQ=255)

                                                 agaATGCCAGCAACTGGTTCACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCgg         <  1:3787096/71‑1 (MQ=255)

                                                        cAGCAACTGGTTCACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATTATc  <  1:1021110/71‑1 (MQ=255)

                                                        cAGCAACTGGTTCACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGATc  <  1:3856473/71‑1 (MQ=255)

AACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGATC  >  minE/1391667‑1391793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`