Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,530,607	G→A	T69I (ACT→ATT)	yfcA ←	conserved inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,530,607	0	G	A	100.0%	115.7 / NA	36	T69I (ACT→ATT)	yfcA	conserved inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/21);  total (15/21)

CATGGCGATATTGAGTTTCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAGTAGCGGAAATAGAGCCGCCGCAGGCTTGCAGTTTATTGGTTGCCAGCGCATT  >  minE/1530546‑1530659                                                              |                                                     cATGGCGATATTGAGTTTCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAATAGCGGaaa                                             >  1:2210337/1‑71 (MQ=255) cATGGCGATATTGAGTTTCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAATAGCGGaaa                                             >  1:3148272/1‑71 (MQ=255) cATGGCGATATTGAGTTTCTGATCGATTAAACTAACCACTTTGCGGCGAATAAAGTAGATAATAGCGGaaa                                             >  1:739745/1‑71 (MQ=255)  aTGGCGATATTGAGTTTCTGATCGCTTAAACTAACCACTTTGCGGCTAATAAAGTAGATAATAGCGGaaa                                             <  1:1255595/70‑1 (MQ=255)  aTGGCGATATTGAGTTTCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAATAGCGGaaa                                             <  1:1247641/70‑1 (MQ=255)  aTGGCGATATTGAGTTTCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAATAGCGGaaa                                             <  1:821287/70‑1 (MQ=255)  aTGGCGATATTGAGTTTCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAATAGCGGaaa                                             <  1:607608/70‑1 (MQ=255)  aTGGCGATATTGAGTTTCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAATAGCGGaaa                                             <  1:521307/70‑1 (MQ=255)  aTGGCGATATTGAGTTTCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAATAGCGGaaa                                             <  1:192800/70‑1 (MQ=255)  aTGGCGATATTGAGTTTCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAATAGCGGaaa                                             <  1:465254/70‑1 (MQ=255)  aTGGCGATATTGAGTTTCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAATAGCGGaaa                                             <  1:274549/70‑1 (MQ=255)     gCGATATTGAGTTTCTTATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAATAGCGGAAATaga                                         <  1:2004793/71‑1 (MQ=255)      cGATATTGAGTTTCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAATAGCGGAAATagag                                        >  1:909012/1‑71 (MQ=255)      cGATATTGAGTTTCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAATAGCGGAAATagag                                        >  1:4076495/1‑71 (MQ=255)        atatTGAGTTTCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAATAGCGGAAATAGAgcc                                      >  1:1533550/1‑71 (MQ=255)        atatTGAGTTTCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAATAGCGGAAATAGAgc                                       >  1:4001528/1‑70 (MQ=255)              aGTTTCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAATAGCGGAAATAGAGCcgccgc                                 <  1:716815/70‑1 (MQ=255)                  tCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAATAGCGGAAATAGAGCCGCCGCAGGc                             >  1:1446588/1‑70 (MQ=255)                  tCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAATAGCGGAAATAGAGCCGCCGCAGGCt                            >  1:338239/1‑71 (MQ=255)                  tCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAATAGCGGAAATAGAGCCGCCGCAGGCt                            >  1:3543942/1‑71 (MQ=255)                  tCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAATAGCGGAAATAGAGCCGCCGCAGGCt                            >  1:3947933/1‑71 (MQ=255)                  tCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAATAGCGGAAATAGAGCCGCCGCAGGCt                            >  1:4217846/1‑71 (MQ=255)                  tCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAATAGCGGAAATAGAGCCGCCGCAGGCt                            >  1:4652523/1‑71 (MQ=255)                  tCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAATAGCGGAAATAGAGCCGCCGCAGGCt                            >  1:4815413/1‑71 (MQ=255)                  tCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAATAGCGGAAATAGAGCCGCCGCAGGCt                            >  1:1875137/1‑71 (MQ=255)                      aTCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAATAGCGGAAATAGAGCCGCCGCAGGCTTg                          <  1:3326685/69‑1 (MQ=255)                      aTCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAATAGCGGAAATAGAGCCGCCGCAGGCTTg                          <  1:114891/69‑1 (MQ=255)                      aTCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAATAGCGGAAATAGAGCCGCCGCAGGCTTg                          <  1:4564089/69‑1 (MQ=255)                      aTCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAATAGCGGAAATAGAGCCGCCGCAGGCTTg                          <  1:2914858/69‑1 (MQ=255)                      aTCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAATAGCGGAAATAGAGCCGCCGCAGGCTTg                          <  1:2949241/69‑1 (MQ=255)                       tCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAATAGCGGAAATAGAGCCGCCGCAGGCTTg                          <  1:1503639/68‑1 (MQ=255)                              aaCTAACCACTTTGCGGCGAATAAAGTAGATAATAGCGGAAATAGGGCCGCCGCAGGCTTGCAGTTTATTg                <  1:3063858/71‑1 (MQ=255)                              aaCTAACCACTTTGCGGCGAATAAAGTAGATAATAGCGGAAATAGAGCCGCCGCAGGCTTGCAGTTTATTg                <  1:2307538/71‑1 (MQ=255)                              aaCTAACCACTTTGCGGCGAATAAAGTAGATAATAGCGGAAATAGAGCCGCCGCAGGCTTGCAGTTTATTg                <  1:4150617/71‑1 (MQ=255)                                           gcggcgAATAAAGTAGATAATAGCGGAAATAGAGCCGCCGCAGGCTTGCAGTTTATTGGTTGCCAgcg      <  1:4545301/68‑1 (MQ=255)                                            cggcgAATAAAGTAGATAATAGCGGAAATAGAGCCGCCGCAGGCTTGCAGTTTATTGGTTGCCAGCGCAtt  <  1:2337662/71‑1 (MQ=255)                                                              |                                                     CATGGCGATATTGAGTTTCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAGTAGCGGAAATAGAGCCGCCGCAGGCTTGCAGTTTATTGGTTGCCAGCGCATT  >  minE/1530546‑1530659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑