Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,634,087	C→T	L75L (CTC→CTT)	acrD →	aminoglycoside/multidrug efflux system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,634,087	0	C	T	100.0%	263.7 / NA	80	L75L (CTC→CTT)	acrD	aminoglycoside/multidrug efflux system
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (44/36);  total (44/36)

GCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCACTTTAAGTTTTAAAGCAGG  >  minE/1634022‑1634152                                                                  |                                                                  gCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTTATGta                                                              <  1:47568/71‑1 (MQ=255) gCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTTATGta                                                              <  1:4006093/71‑1 (MQ=255) gCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTTATGta                                                              <  1:3024552/71‑1 (MQ=255)  cccAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTTATGta                                                              <  1:1207908/70‑1 (MQ=255)      gACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTTATGTATATGt                                                         >  1:975123/1‑71 (MQ=255)      gACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTTATGTATATGt                                                         <  1:883351/71‑1 (MQ=255)      gACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTTATGTATATGt                                                         >  1:772135/1‑71 (MQ=255)      gACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTTATGTATATGt                                                         <  1:4829931/71‑1 (MQ=255)      gACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTTATGTATATGt                                                         <  1:4693197/71‑1 (MQ=255)      gACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTTATGTATATGt                                                         >  1:4094320/1‑71 (MQ=255)      gACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTTATGTATATGt                                                         <  1:3804264/71‑1 (MQ=255)      gACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTTATGTATATGt                                                         <  1:2991656/71‑1 (MQ=255)      gACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTTATGTATATGt                                                         <  1:2859226/71‑1 (MQ=255)         gCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTTATGTATATGTCa                                                       >  1:1183881/1‑70 (MQ=255)         gCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTTATGTATATGTCa                                                       >  1:405198/1‑70 (MQ=255)         gCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTTATGTATATGTCa                                                       >  1:1665558/1‑70 (MQ=255)         gCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTTATGTATATGTCAt                                                      <  1:395668/71‑1 (MQ=255)         gCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTTATGTATATGTCAt                                                      <  1:895345/71‑1 (MQ=255)         gCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTTATGTATATGTCAt                                                      <  1:3895251/71‑1 (MQ=255)         gCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTTATGTATATGTCAt                                                      >  1:3884987/1‑71 (MQ=255)         gCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTTATGTATATGTCAt                                                      >  1:671891/1‑71 (MQ=255)         gCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTTATGTATATGTCAt                                                      <  1:2800273/71‑1 (MQ=255)         gCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTTATGTATATGTCAt                                                      >  1:2949398/1‑71 (MQ=255)         gCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTTATGTATATGTCAt                                                      >  1:3016660/1‑71 (MQ=255)         gCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTTATGTATATGTCAt                                                      <  1:4547427/71‑1 (MQ=255)              aaaaCACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTTATGTATATGt                                                         <  1:1458329/63‑1 (MQ=255)                  cacCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTTATGTATATGTCATCTCagag                                               >  1:1754801/1‑69 (MQ=255)                  cacCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTTATGTATATGTCATCTCagag                                               >  1:1943300/1‑69 (MQ=255)                                ttATCGAGCAAAATATGACCGGCCTCGATAATCTTATGTATATGTCCTCTCATAGCa                                             <  1:4703689/57‑1 (MQ=255)                                ttATCGAGCAAAATATGACCGGCCTCGATAATCTTATGTATATGTCATCTCAGAGCa                                             <  1:3215047/57‑1 (MQ=255)                                ttATCGAGCAAAATATGACCGGCCTCGATAATCTTATGTATATGTCATCTCAGAGCa                                             <  1:1305779/57‑1 (MQ=255)                                ttATCGAGCAAAATATGACCGGCCTCGATAATCTTATGTATATGTCATCTCAGAGCa                                             <  1:4057523/57‑1 (MQ=255)                                ttATCGAGCAAAATATGACCGGCCTCGATAATCTTATGTATATGTCATCTCAGAGCa                                             <  1:1637646/57‑1 (MQ=255)                                ttATCGAGCAAAATATGACCGGCCTCGATAATCTTATGTATATGTCATCTCAGAGCa                                             <  1:3153573/57‑1 (MQ=255)                                ttATCGAGCAAAATATGACCGGCCTCGATAATCTTATGTATATGTCATCTCAGAGCa                                             <  1:3086962/57‑1 (MQ=255)                                ttATCGAGCAAAATATGACCGGCCTCGATAATCTTATGTATATGTCATCTCAGAGCa                                             <  1:1947657/57‑1 (MQ=255)                                     gAGCAAAATATGACCGGCCTCGATAATCTTATGTATATGTCATCTCa                                                  >  1:4705150/1‑47 (MQ=255)                                     gAGCAAAATATGACCGGCCTCGATAATCTTATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATc                          >  1:4274705/1‑71 (MQ=255)                                     gAGCAAAATATGACCGGCCTCGATAATCTTATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATc                          >  1:649417/1‑71 (MQ=255)                                     gAGCAAAATATGACCGGCCTCGATAATCTTATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATc                          >  1:721278/1‑71 (MQ=255)                                          aaaTATGACCGGCCTCGATAATCTTATGTATATGTCATCTCAGAGCa                                             <  1:4638736/47‑1 (MQ=255)                                           aaTATGACCGGCCTCGATAATCTTATGTATATGTCATCTCAGAGCAGTGGCACCg                                    >  1:273891/1‑55 (MQ=255)                                           aaTATGACCGGCCTCGATAATCTTATGTATATGTCATCTCAGAGCAGTGGCACCg                                    >  1:959917/1‑55 (MQ=255)                                            atatGACCGGCCTCGATAATCTTATGTATATGTCATCTCAGAGCa                                             <  1:738021/45‑1 (MQ=255)                                               tGACCGGCCTCGATAATCTTATGTATATGTCAtctc                                                   >  1:939573/1‑36 (MQ=255)                                               tGACCGGCCTCGATAATCTTATGTATATGTCATCTCAGAGCAGTGGCACCgg                                   >  1:3097404/1‑52 (MQ=255)                                               tGACCGGCCTCGATAATCTTATGTATATGTCATCTCAGAGCAGTGGCACCGGt                                  >  1:1122834/1‑53 (MQ=255)                                                    ggCCTCGATAATCTTATGTATATGTCATCTCAGAGCAGGGGCACCg                                    <  1:1225173/46‑1 (MQ=255)                                                     gCCTCGATAATCTTATGTATATGTCATCTCAGAGCa                                             <  1:642819/36‑1 (MQ=255)                                                     gCCTCGATAATCTTATGTATATGTCATCTCAGAGCAGTGGCACCGGTCa                                >  1:3601514/1‑49 (MQ=255)                                                     gCCTCGATAATCTTATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCACTTTAAGttt           >  1:3928543/1‑70 (MQ=255)                                                     gCCTCGATAATCTTATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCACTTTAAGttt           >  1:2723914/1‑70 (MQ=255)                                                     gCCTCGATAATCTTATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCACTTTAAGttt           >  1:2473692/1‑70 (MQ=255)                                                     gCCTCGATAATCTTATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCACTTTAAGttt           >  1:3450586/1‑70 (MQ=255)                                                     gCCTCGATAATCTTATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCACTTTAAGttt           >  1:2433883/1‑70 (MQ=255)                                                     gCCTCGATAATCTTATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCACTTTAAGttt           >  1:4671850/1‑70 (MQ=255)                                                     gCCTCGATAATCTTATGTATATGTCATCTCAGAGCAGTGGCACCCGTCAGGCATCTGTCACTTTAAGttt           >  1:3549761/1‑70 (MQ=255)                                                        tCGATAATCTTATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCACTTTAAGtttt          >  1:4415461/1‑68 (MQ=255)                                                        tCGATAATCTTATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCACTTTAAGTTTTa         >  1:4494806/1‑69 (MQ=255)                                                        tCGATAATCTTATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCACTTTAAGTTTTa         >  1:3347087/1‑69 (MQ=255)                                                        tCGATAATCTTATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCACTTTAAGTTTTa         >  1:4392503/1‑69 (MQ=255)                                                        tCGATAATCTTATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCACTTTAAGTTTTa         >  1:2105315/1‑69 (MQ=255)                                                        tCGATAATCTTATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCACTTTAAGTTTTa         >  1:507986/1‑69 (MQ=255)                                                          gATAATCTTATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCACttt                 <  1:4019091/59‑1 (MQ=255)                                                          gATAATCTTATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCACttt                 <  1:1273016/59‑1 (MQ=255)                                                          gATAATCTTATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCACttt                 <  1:2403597/59‑1 (MQ=255)                                                          gATAATCTTATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCACttt                 <  1:2380195/59‑1 (MQ=255)                                                          gATAATCTTATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCACttt                 <  1:3099560/59‑1 (MQ=255)                                                          gATAATCTTATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCACttt                 <  1:4350557/59‑1 (MQ=255)                                                          gATAATCTTATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCACttt                 <  1:3635355/59‑1 (MQ=255)                                                          gATAATCTTATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCACTTTAAGTTTTAAAg      >  1:135117/1‑70 (MQ=255)                                                          gATAATCTTATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCACTTTAAGTTTTAAAg      >  1:2032353/1‑70 (MQ=255)                                                          gATAATCTTATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCACTTTAAGTTTTAAAg      >  1:2784414/1‑70 (MQ=255)                                                          gATAATCTTATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCACTTTAAGTTTTAAAg      >  1:1413006/1‑70 (MQ=255)                                                          gATAATCTTATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCACTTTAAGTTTTAAAg      >  1:426380/1‑70 (MQ=255)                                                           aTAATCTTATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCACttt                 <  1:4243466/58‑1 (MQ=255)                                                              aTCTTATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCACTTTAAGTTTTAAAGCAgg  >  1:322067/1‑70 (MQ=255)                                                              aTCTTATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCACTTTAAGTTTTAAAGCAgg  >  1:3281188/1‑70 (MQ=255)                                                              aTCTTATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCACTTTAAGTTTTAAAGCAgg  >  1:3297539/1‑70 (MQ=255)                                                              aTCTTATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCACTTTAAGTTTTAAAGCAgg  >  1:4416868/1‑70 (MQ=255)                                                                  |                                                                  GCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCACTTTAAGTTTTAAAGCAGG  >  minE/1634022‑1634152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑