Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 1,657,320 A→G S531G (AGT→GGT)  ppk → polyphosphate kinase, component of RNA degradosome

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,657,3200AG100.0% 250.1 / NA 67S531G (AGT→GGT) ppkpolyphosphate kinase, component of RNA degradosome
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (40/27);  total (40/27)

CTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGTTGATCGTCTGT  >  minE/1657266‑1657377
                                                      |                                                         
cTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCGGTGGTATCa                                                  <  1:56028/64‑1 (MQ=255)
cTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCGGTGGTATCa                                                  <  1:1341127/64‑1 (MQ=255)
cTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCGGTGGTATCa                                                  <  1:3103230/64‑1 (MQ=255)
cTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCGGTGGTATCa                                                  <  1:3872178/64‑1 (MQ=255)
cTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCGGTGGTATCACCCTg                                             >  1:2367534/1‑69 (MQ=255)
cTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCGGTGGTATCACCCTg                                             >  1:307944/1‑69 (MQ=255)
cTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCGGTGGTATCACCCTg                                             >  1:3102239/1‑69 (MQ=255)
cTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCGGTGGTATCACCCTg                                             >  1:2167126/1‑69 (MQ=255)
cTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCGGTGGTATCACCCTg                                             >  1:1115534/1‑69 (MQ=255)
cTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCGGTGGTATCACCCTg                                             >  1:3534258/1‑69 (MQ=255)
cTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCGGTGGTATCACCCTg                                             >  1:2820679/1‑69 (MQ=255)
cTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCGGTGGTATCACCCTg                                             >  1:4578810/1‑69 (MQ=255)
cTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCGGTGGTATCACCCTg                                             >  1:4597003/1‑69 (MQ=255)
cTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCGGTGGTATCACCCTg                                             >  1:4786776/1‑69 (MQ=255)
cTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCGGTGGTATCACCCTg                                             >  1:4851434/1‑69 (MQ=255)
cTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCGGTGGTATCACCCTg                                             >  1:112824/1‑69 (MQ=255)
  aTTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCGGTGGTATCa                                                  <  1:3746912/62‑1 (MQ=255)
  aTTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCGGTGGTATCAcc                                                >  1:3432524/1‑64 (MQ=255)
  aTTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCGGTGGTATCAcc                                                >  1:4144696/1‑64 (MQ=255)
  aTTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCGGTGGTATCAcc                                                >  1:2757623/1‑64 (MQ=255)
  aTTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCGGTGGTATCAcc                                                >  1:1357628/1‑64 (MQ=255)
  aTTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCGGTGGTATCAcc                                                >  1:1250318/1‑64 (MQ=255)
      tATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCGGTGGTATCACCCTGAAGCTaaa                                     >  1:3150893/1‑71 (MQ=255)
                  gACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCGGTGGTATCACCCTGAAGCTAAATAACCTTGTCg                          <  1:4171571/70‑1 (MQ=255)
                   aCCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCGGTGGTATCa                                                  <  1:2955203/45‑1 (MQ=255)
                   aCCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCGGTGGTATCACCCTGAAGCTAAATAACCTTGTCg                          <  1:698945/69‑1 (MQ=255)
                    ccGCGAGATCGCCAACGCGCAGCAAGGGCTGCCGGGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATa                       <  1:3972151/71‑1 (MQ=255)
                    ccGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCGGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATa                       <  1:2674178/71‑1 (MQ=255)
                    ccGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCGGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATa                       <  1:1899877/71‑1 (MQ=255)
                    ccGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCGGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATa                       <  1:1307088/71‑1 (MQ=255)
                    ccGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCGGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATa                       <  1:2956789/71‑1 (MQ=255)
                           aTCGCCAACGCGCAGCAAGGGCTGCCCGGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCt                <  1:3229464/71‑1 (MQ=255)
                           aTCGCCAACGCGCAGCAAGGGCTGCCCGGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCt                <  1:607156/71‑1 (MQ=255)
                           aTCGCCAACGCGCAGCAAGGGCTGCCCGGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCt                <  1:308456/71‑1 (MQ=255)
                           aTCGCCAACGCGCAGCAAGGGCTGCCCGGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCt                <  1:1518523/71‑1 (MQ=255)
                           aTCGCCAACGCGCAGCAAGGGCTGCCCGGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCt                <  1:4563910/71‑1 (MQ=255)
                           aTCGCCAACGCGCAGCAAGGGCTGCCCGGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCt                <  1:1728980/71‑1 (MQ=255)
                           aTCGCCAACGCGCAGCAAGGGCTGCCCGGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCt                <  1:4097452/71‑1 (MQ=255)
                           aTCGCCAACGCGCAGCAAGGGCTGCCCGGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCt                <  1:3968559/71‑1 (MQ=255)
                            tCGCCAACGCGCAGCAAGGGCTGCCCGGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCt                <  1:2178701/70‑1 (MQ=255)
                            tCGCCAACGCGCAGCAAGGGCTGCCCGGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCt                <  1:4006859/70‑1 (MQ=255)
                             cGCCAACGCGCAGCAAGGGCTGCCCGGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTgg              >  1:791866/1‑71 (MQ=255)
                             cGCCAACGCGCAGCAAGGGCTGCCCGGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTgg              >  1:931370/1‑71 (MQ=255)
                             cGCCAACGCGCAGCAAGGGCTGCCCGGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTgg              >  1:3288805/1‑71 (MQ=255)
                             cGCCAACGCGCAGCAAGGGCTGCCCGGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTgg              >  1:1849857/1‑71 (MQ=255)
                             cGCCAACGCGCAGCAAGGGCTGCCCGGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTgg              >  1:35171/1‑71 (MQ=255)
                             cGCCAACGCGCAGCAAGGGCTGCCCGGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTgg              >  1:3488309/1‑71 (MQ=255)
                                  aCGCGCAGCAAGGGCTGCCCGGTGGTATCACCCTGAAGCTAAATAACCTTGTCg                          <  1:4787075/54‑1 (MQ=255)
                                    gcgcAGCAAGGGCTGCCCGGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGtt            >  1:603581/1‑66 (MQ=255)
                                    gcgcAGCAAGGGCTGCCCGGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGtt            >  1:3768267/1‑66 (MQ=255)
                                    gcgcAGCAAGGGCTGCCCGGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGtt            >  1:3733742/1‑66 (MQ=255)
                                    gcgcAGCAAGGGCTGCCCGGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGtt            >  1:4482041/1‑66 (MQ=255)
                                    gcgcAGCAAGGGCTGCCCGGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGtt            >  1:194465/1‑66 (MQ=255)
                                    gcgcAGCAAGCGCTGCCCGGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGt             >  1:2531222/1‑65 (MQ=255)
                                         gcaAGGGCTGCCCGGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGTTGATCGTCTGt  <  1:2134514/71‑1 (MQ=255)
                                         gcaAGGGCTGCCCGGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGTTGATAGTCTGt  <  1:8207/71‑1 (MQ=255)
                                          caAGGGCTGCCCGGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGTTGATCGTCTGt  <  1:2284754/70‑1 (MQ=255)
                                                cTGCCCGGTGGTATCACCCTGAAGCTAAATACCCTTGTCGATAAAGGCCTGGt             >  1:3757281/1‑53 (MQ=255)
                                                cTGCCCGGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGt             >  1:4720566/1‑53 (MQ=255)
                                                cTGCCCGGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGt             >  1:1587730/1‑53 (MQ=255)
                                                cTGCCCGGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGt             >  1:3338941/1‑53 (MQ=255)
                                                cTGCCCGGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGt             >  1:4860179/1‑53 (MQ=255)
                                                cTGCCCGGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGt             >  1:3813645/1‑53 (MQ=255)
                                                cTGCCCGGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGt             >  1:620123/1‑53 (MQ=255)
                                                cTGCCCGGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGt             >  1:2168261/1‑53 (MQ=255)
                                                cTGCCCGGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGt             >  1:716639/1‑53 (MQ=255)
                                                cTGCCCGGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGt             >  1:1760075/1‑53 (MQ=255)
                                                      |                                                         
CTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGTTGATCGTCTGT  >  minE/1657266‑1657377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑