BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,670,644	A→G	I232T (ATT→ACT)	yfgL ←	protein assembly complex, lipoprotein component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,670,644	0	A	G	100.0%	456.5 / NA	121	I232T (ATT→ACT)	yfgL	protein assembly complex, lipoprotein component
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (48/73); total (48/73)

Only 100 of 121 total aligned reads displayed.

CAACGACGGGAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATT  >  minE/1670579‑1670702

caacGACGGGAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTa                                                          <  1:242063/68‑1 (MQ=255)

caacGACGGGAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTa                                                          <  1:3574224/68‑1 (MQ=255)

 aacGACGGGAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTa                                                          <  1:272166/67‑1 (MQ=255)

      cgGGAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTaag                                                        >  1:3154952/1‑62 (MQ=255)

      cgGGAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCtgctgc                                                 >  1:808328/1‑71 (MQ=255)

      cgGGAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCtgctgc                                                 >  1:2444503/1‑71 (MQ=255)

      cgGGAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCtgctgc                                                 >  1:2789737/1‑71 (MQ=255)

      cgGGAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCtgctgc                                                 >  1:345409/1‑71 (MQ=255)

      cgGGAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCtgctgc                                                 >  1:1527664/1‑71 (MQ=255)

      cgGGAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCtgctgc                                                 >  1:3669483/1‑71 (MQ=255)

      cgGGAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCtgctgc                                                 >  1:1060008/1‑71 (MQ=255)

            tCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAatc                                           <  1:3834615/71‑1 (MQ=255)

            tCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAatc                                           <  1:2561251/71‑1 (MQ=255)

            tCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAatc                                           <  1:1868327/71‑1 (MQ=255)

             cGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAatca                                          >  1:3615429/1‑71 (MQ=255)

             cGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAatca                                          >  1:3780377/1‑71 (MQ=255)

             cGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAatca                                          >  1:2507924/1‑71 (MQ=255)

             cGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAatca                                          >  1:4241706/1‑71 (MQ=255)

             cGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAatca                                          >  1:2722317/1‑71 (MQ=255)

             cGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAatca                                          >  1:4454308/1‑71 (MQ=255)

             cGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAatca                                          >  1:2884361/1‑71 (MQ=255)

             cGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAatca                                          >  1:2965641/1‑71 (MQ=255)

             cGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAatc                                           <  1:3695635/70‑1 (MQ=255)

             cGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAatc                                           >  1:1413665/1‑70 (MQ=255)

              gtgtCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAatca                                          <  1:3033867/70‑1 (MQ=255)

              gtgtCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAatca                                          <  1:549710/70‑1 (MQ=255)

              gtgtCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAatca                                          <  1:3215415/70‑1 (MQ=255)

              gtgtCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAatca                                          <  1:560874/70‑1 (MQ=255)

              gtgtCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAatca                                          <  1:3274943/70‑1 (MQ=255)

              gtgtCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAatca                                          <  1:3491006/70‑1 (MQ=255)

              gtgtCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAatca                                          <  1:895555/70‑1 (MQ=255)

              gtgtCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAatca                                          <  1:2048864/70‑1 (MQ=255)

              gtgtCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAatca                                          <  1:1993311/70‑1 (MQ=255)

              gtgtCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAatca                                          <  1:1911876/70‑1 (MQ=255)

              gtgtCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAatca                                          <  1:1912989/70‑1 (MQ=255)

                    aCATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTggtc                                   <  1:1439971/71‑3 (MQ=255)

                    aCATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGcc                                   <  1:1259979/71‑1 (MQ=255)

                    aCATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGcc                                   <  1:2233590/71‑1 (MQ=255)

                    aCATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGcc                                   <  1:616036/71‑1 (MQ=255)

                    aCATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGcc                                   <  1:1727540/71‑1 (MQ=255)

                    aCATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGcc                                   <  1:1891974/71‑1 (MQ=255)

                    aCATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGcc                                   <  1:599803/71‑1 (MQ=255)

                     cATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTgg                                     >  1:804425/1‑68 (MQ=255)

                     cATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGccc                                  >  1:4843890/1‑71 (MQ=255)

                     cATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGccc                                  >  1:401823/1‑71 (MQ=255)

                     cATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGccc                                  >  1:1241794/1‑71 (MQ=255)

                     cATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGccc                                  >  1:4281605/1‑71 (MQ=255)

                     cATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGccc                                  >  1:518212/1‑71 (MQ=255)

                     cATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGccc                                  >  1:231274/1‑71 (MQ=255)

                     cATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGccc                                  >  1:663180/1‑71 (MQ=255)

                     cATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGccc                                  >  1:61576/1‑71 (MQ=255)

                     cATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGccc                                  >  1:2008695/1‑71 (MQ=255)

                     cATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGccc                                  >  1:258748/1‑71 (MQ=255)

                     cATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCAAAATCATCTGGacc                                  >  1:3940079/1‑71 (MQ=255)

                     cATCGCTCAGACGGT‑AATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCt                                       >  1:3987687/1‑65 (MQ=255)

                     cATCGCTCACACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGccc                                  >  1:1018075/1‑71 (MQ=255)

                     cATAGCTCAGACGGTCAAATTCGGTAGAACCGGTCGCCTGGGAAGTACgctg                                                     >  1:4789920/1‑52 (MQ=255)

                       tCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGCCCTg                                <  1:113527/71‑1 (MQ=255)

                       tCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGCCCTg                                <  1:4095826/71‑1 (MQ=255)

                       tCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGCCCTg                                <  1:4752996/71‑1 (MQ=255)

                             aGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTa                                                          <  1:2869310/39‑1 (MQ=255)

                             aGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGCCCTGTTCCAt                          >  1:1201685/1‑71 (MQ=255)

                             aGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGCCCTGTTCCAt                          >  1:1050856/1‑71 (MQ=255)

                             aGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGCCCTGTTCCAt                          >  1:2116615/1‑71 (MQ=255)

                             aGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGCCCTGTTCCAt                          >  1:3683635/1‑71 (MQ=255)

                             aGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGCCCTGTTCCAt                          >  1:2930430/1‑71 (MQ=255)

                                cGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAg                       <  1:1534330/71‑1 (MQ=255)

                                cGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAg                       <  1:4500620/71‑1 (MQ=255)

                                 ggTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGc                      <  1:1443290/71‑1 (MQ=255)

                                   tCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAatca                                          <  1:2049014/49‑1 (MQ=255)

                                   tCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGCCCTGTTCCGTCAgagc                    <  1:4094133/71‑4 (MQ=255)

                                   tCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGCCCTGTTCCGTCAGcgc                    <  1:4293523/71‑3 (MQ=255)

                                   tCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGCCCTGTTCCGTCAGcgc                    <  1:2486286/71‑3 (MQ=255)

                                   tCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGCCCTGTTCCGTCAGcgc                    <  1:268628/71‑3 (MQ=255)

                                   tCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGCCCTGTTCCGTCAGcgc                    <  1:1084328/71‑3 (MQ=255)

                                   tCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGCCCTGTTCCGTCAGcgc                    <  1:627315/71‑3 (MQ=255)

                                   tCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGCCCTGTTCCGTCAGcgc                    <  1:21600/71‑3 (MQ=255)

                                   tCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGCCCTGTTCCGTCAGcgc                    <  1:4312092/71‑3 (MQ=255)

                                   tCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGCCCTGTTCCGTCAGcgc                    <  1:1679112/71‑3 (MQ=255)

                                   tCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGCCCTGTTCCGTCAGcgc                    <  1:1653730/71‑3 (MQ=255)

                                   tCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGCCCTGTTCCGTCAGcgc                    <  1:4027190/71‑3 (MQ=255)

                                   tCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGCCCTGTTCCGTCAGcgc                    <  1:4021533/71‑3 (MQ=255)

                                   tCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGCCCTGTTCCGTCAGcgc                    <  1:1630898/71‑3 (MQ=255)

                                   tCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGCCCTGTTCCGTCAGcgc                    <  1:132502/71‑3 (MQ=255)

                                   tCAATATCGGTAGAACCGGTCGCCTGGTAAGTACGCTGCTGCCAAATCATCTGGCCCTGTTCCGTAAGcgc                    <  1:3885377/71‑3 (MQ=255)

                                     aaTTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTg                  >  1:1760104/1‑71 (MQ=255)

                                     aaTTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTg                  >  1:2258798/1‑71 (MQ=255)

                                       tttCGGTAGAACCGGTCGCCTTGGAAGTACGCTGCTGCCAAatca                                          <  1:1968350/45‑1 (MQ=38)

                                         tCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCCTCTGGCCCTGtt                              >  1:2652294/1‑55 (MQ=255)

                                         tCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCt              >  1:3126589/1‑71 (MQ=255)

                                         tCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCt              >  1:1981782/1‑71 (MQ=255)

                                         tCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTCCCAAATCATCTGGCCCTGt                               >  1:3968250/1‑54 (MQ=255)

                                          cGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCt              <  1:3772353/70‑1 (MQ=255)

                                          cGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCt              <  1:2083378/70‑1 (MQ=255)

                                          cGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCt              <  1:51161/70‑1 (MQ=255)

                                          cGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCt              <  1:2740893/70‑1 (MQ=255)

                                          cGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCt              <  1:1965063/70‑1 (MQ=255)

                                          cGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGAACTGCGCt              <  1:4495487/70‑1 (MQ=255)

                                                     gTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCAtt  <  1:1339169/71‑1 (MQ=255)

                                                          cTGGGAAGTACGCTGCTGCCAAATCATCTGGCCCTGTTCCGTCAGcgc                    <  1:727518/48‑3 (MQ=38)

CAACGACGGGAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATT  >  minE/1670579‑1670702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`