BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,833,578	T→C	L107L (CTT→CTC)	ygbL →	predicted class II aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,833,578	0	T	C	100.0%	387.3 / NA	102	L107L (CTT→CTC)	ygbL	predicted class II aldolase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (47/55); total (47/55)

Only 100 of 102 total aligned reads displayed.

CGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTCACACCATACGTGGTGATGCGGA  >  minE/1833511‑1833645

cgcTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTcc                                                                  <  1:95803/71‑1 (MQ=255)

cgcTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTcc                                                                  <  1:2843231/71‑1 (MQ=255)

cgcTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTcc                                                                  <  1:302802/71‑1 (MQ=255)

cgcTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTcc                                                                  <  1:3175627/71‑1 (MQ=255)

cgcTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTcc                                                                  <  1:253744/71‑1 (MQ=255)

cgcTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTcc                                                                  <  1:3746341/71‑1 (MQ=255)

cgcTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTcc                                                                  <  1:421747/71‑1 (MQ=255)

cgcTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTcc                                                                  <  1:234262/71‑1 (MQ=255)

cgcTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTcc                                                                  <  1:4419067/71‑1 (MQ=255)

cgcTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTcc                                                                  <  1:2237629/71‑1 (MQ=255)

cgcTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTcc                                                                  <  1:2223231/71‑1 (MQ=255)

cgcTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTcc                                                                  <  1:2134656/71‑1 (MQ=255)

cgcTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTcc                                                                  <  1:4566267/71‑1 (MQ=255)

cgcTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTcc                                                                  <  1:1300038/71‑1 (MQ=255)

cgcTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTcc                                                                  <  1:684066/71‑1 (MQ=255)

cgcTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTcc                                                                  <  1:4923900/71‑1 (MQ=255)

cgcTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTcc                                                                  <  1:896044/71‑1 (MQ=255)

cgcTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTcc                                                                  <  1:4768379/71‑1 (MQ=255)

cgcTGTATCGCAACAATCCGCGCTGTAAAGCGGGGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTcc                                                                  <  1:4882155/71‑1 (MQ=255)

     tATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTcctgcct                                                             >  1:2559962/1‑71 (MQ=255)

     tATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTcctgcct                                                             >  1:1293452/1‑71 (MQ=255)

     tATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTcctgcct                                                             >  1:4694151/1‑71 (MQ=255)

     tATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTcctgcct                                                             >  1:4587064/1‑71 (MQ=255)

      aTCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTcctgcctg                                                            >  1:4640100/1‑71 (MQ=255)

       tCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTCctgcctgc                                                           <  1:2628625/71‑1 (MQ=255)

       tCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTCctgcctgc                                                           <  1:4127756/71‑1 (MQ=255)

       tCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTCctgcctgc                                                           <  1:3200682/71‑1 (MQ=255)

       tCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTCctgcctgc                                                           <  1:1663412/71‑1 (MQ=255)

                tCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTg                                                   >  1:2892444/1‑70 (MQ=255)

                tCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTg                                                   >  1:3074085/1‑70 (MQ=255)

                tCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTg                                                   >  1:821117/1‑70 (MQ=255)

                tCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTg                                                   >  1:1162056/1‑70 (MQ=255)

                tCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTg                                                   >  1:2725791/1‑70 (MQ=255)

                tCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTg                                                   >  1:3312876/1‑70 (MQ=255)

                tCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTg                                                   >  1:3917881/1‑70 (MQ=255)

                tCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTg                                                   >  1:614369/1‑70 (MQ=255)

                tCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTg                                                   >  1:4816768/1‑70 (MQ=255)

                tCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTg                                                   >  1:4549937/1‑70 (MQ=255)

                tCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTg                                                   >  1:456267/1‑70 (MQ=255)

                tCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTg                                                   >  1:4643801/1‑70 (MQ=255)

                tCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTg                                                   >  1:4649642/1‑70 (MQ=255)

                tCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTg                                                   >  1:1399993/1‑70 (MQ=255)

                tCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTg                                                   >  1:1558441/1‑70 (MQ=255)

                tCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTg                                                   >  1:2532007/1‑70 (MQ=255)

                tCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTg                                                   >  1:2565697/1‑70 (MQ=255)

                tCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTg                                                   >  1:2581932/1‑70 (MQ=255)

                tCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTg                                                   >  1:1643763/1‑70 (MQ=255)

                tCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTg                                                   >  1:2594092/1‑70 (MQ=255)

                tCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTg                                                   >  1:1381996/1‑70 (MQ=255)

                tCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTg                                                   >  1:11711/1‑70 (MQ=255)

                tCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTg                                                   >  1:2653794/1‑70 (MQ=255)

                tCCGCGCTGTAAAGCGGTGGTG‑ATTTGCACAGCACATGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTg                                                   >  1:2781521/1‑69 (MQ=255)

                    cgcTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTGGAcag                                              <  1:2131422/71‑1 (MQ=255)

                    cgcTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTGGAcag                                              <  1:2728862/71‑1 (MQ=255)

                    cgcTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTGGAcag                                              <  1:4063156/71‑1 (MQ=255)

                    cgcTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTGGAcag                                              <  1:1501141/71‑1 (MQ=255)

                     gcTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTGGAcag                                              <  1:804026/70‑1 (MQ=255)

                         tAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTGGACagcagc                                          >  1:1191678/1‑70 (MQ=255)

                                 tggtgCATTTGCACAGCACATGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTAtt                                 <  1:3218420/71‑1 (MQ=255)

                                   gtgCATTTGCACAGCACATGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTc                                <  1:3222435/70‑1 (MQ=255)

                                   gtgCATTTGCACAGCACATGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTc                                <  1:2528871/70‑1 (MQ=255)

                                   gtgCATTTGCACAGCACATGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTc                                <  1:4553983/70‑1 (MQ=255)

                                     gCATTTGCACAGCACATGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTc                             >  1:2988715/1‑71 (MQ=255)

                                      cATTTGCACAGCACATGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTGGAcag                                              <  1:3318918/53‑1 (MQ=255)

                                      cATTTGCACAGCACATGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTc                             <  1:2216320/70‑1 (MQ=255)

                                      cATTTGCACAGCACATGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTc                             <  1:2593934/70‑1 (MQ=255)

                                          tGCACAGCACATGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGtt                         >  1:14668/1‑70 (MQ=255)

                                          tGCACAGCACATGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGtt                         >  1:589640/1‑70 (MQ=255)

                                          tGCACAGCACATGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGtt                         >  1:1767081/1‑70 (MQ=255)

                                          tGCACAGCACATGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGtt                         >  1:1015326/1‑70 (MQ=255)

                                          tGCACAGCACATGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGtt                         >  1:4337006/1‑70 (MQ=255)

                                          tGCACAGCACATGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGtt                         >  1:243195/1‑70 (MQ=255)

                                          tGCACAGCACATGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGtt                         >  1:3124488/1‑70 (MQ=255)

                                          tGCACAGCACATGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGtt                         >  1:3118938/1‑70 (MQ=255)

                                          tGCACAGCACATGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGtt                         >  1:2370299/1‑70 (MQ=255)

                                          tGCACAGCACATGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGt                          >  1:2634318/1‑69 (MQ=255)

                                          tGCACAGCACATGGACGACGGCGCTCTCCTGCCTGCAAGGGCTGGACAGCAGCAACGtt                                    >  1:4410681/1‑59 (MQ=255)

                                              cagcacaTGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTCacac                    <  1:3277795/71‑1 (MQ=255)

                                              cagcacaTGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTCacac                    <  1:1621756/71‑1 (MQ=255)

                                              cagcacaTGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTCacac                    <  1:2374591/71‑1 (MQ=255)

                                              cagcacaTGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTCacac                    <  1:4639385/71‑1 (MQ=255)

                                              cagcacaTGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTCacac                    <  1:794852/71‑1 (MQ=255)

                                              cagcacaTGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTCacac                    <  1:4295069/71‑1 (MQ=255)

                                              cagcacaTGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTCacac                    <  1:2744492/71‑1 (MQ=255)

                                              cagcacaTGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTCacac                    <  1:4298061/71‑1 (MQ=255)

                                              cagcacaTGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTCacac                    <  1:2670718/71‑1 (MQ=255)

                                              cagcacaTGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTCacac                    <  1:914289/71‑1 (MQ=255)

                                                     tGGTCGACGGCGCTCTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTCACACCATACgt             <  1:400337/71‑1 (MQ=255)

                                                     tGGTCGACGGCGCTCTCCTGCCTGCAAGAGCTGGACAGCAGCAACGTTATTCGTCCGTTCACACCATACgt             <  1:2635195/71‑1 (MQ=255)

                                                        tCGACGGCGCTCTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTCACACCATACgtggt          <  1:2308228/71‑1 (MQ=255)

                                                        tCGACGGCGCTCTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTCACACCATACgtggt          <  1:1678636/71‑1 (MQ=255)

                                                        tCGACGGCGCTCTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTCACACCATACgtggt          <  1:1822275/71‑1 (MQ=255)

                                                        tCGACGGCGCTCTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTCACACCATACgtggt          <  1:4557757/71‑1 (MQ=255)

                                                          gACGGCGCTCTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTCACACCATACgtggtg         <  1:926475/70‑1 (MQ=255)

                                                          gACGGCGCTCTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTCACACCATACGTGGTGa        <  1:1797017/71‑1 (MQ=255)

                                                          gACGGCGCTCTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTCACACCATACGTGGTGa        <  1:4546274/71‑1 (MQ=255)

                                                                gcTCTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTCACACCATACGTGGTGATGCGGa  >  1:4853236/1‑71 (MQ=255)

                                                                gcTCTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTCACACCATACGTGGTGATGCGGa  >  1:1776894/1‑71 (MQ=255)

                                                                gcTCTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTCACACCATACGTGGTGATGCGGa  >  1:2805856/1‑71 (MQ=255)

                                                                gcTCTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTCACACCATACGTGGTGATGCGGa  >  1:271270/1‑71 (MQ=255)

CGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTCACACCATACGTGGTGATGCGGA  >  minE/1833511‑1833645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`