| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 1,889,046 | G→A | P395S (CCC→TCC) | gudX ← | predicted glucarate dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,889,046 | 0 | G | A | 85.7% | 233.4 / 28.1 | 91 | P395S (CCC→TCC) | gudX | predicted glucarate dehydratase |
| Reads supporting (aligned to +/- strand): ref base G (13/0); new base A (27/51); total (40/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.28e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.93e-01 | |||||||||||
TTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGCCC > minE/1888987‑1889113 | tttATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGACGCATCAGGaa > 1:1913389/1‑71 (MQ=255)tttATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGACGCATCAGGaa > 1:652130/1‑71 (MQ=255) tataGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCAt > 1:3803117/1‑63 (MQ=255) tataGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCAt > 1:4158740/1‑63 (MQ=255) tataGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCAt > 1:3341124/1‑63 (MQ=255) tataGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCAt > 1:3283461/1‑63 (MQ=255) tataGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCAt > 1:4027301/1‑63 (MQ=255) tataGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCAt > 1:3839268/1‑63 (MQ=255) tataGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCAt > 1:1031946/1‑63 (MQ=255) tataGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCAt > 1:1344005/1‑63 (MQ=255) tataGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCAt > 1:3612081/1‑63 (MQ=255) tataGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCAt > 1:1266762/1‑63 (MQ=255) tataGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCAt > 1:760144/1‑63 (MQ=255) tataGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCAt > 1:3607541/1‑63 (MQ=255) tataGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCAt > 1:868296/1‑63 (MQ=255) tataGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGACGCATCAGGAACg > 1:2689616/1‑71 (MQ=255) tataGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGACGCATCAGGAACg > 1:2098791/1‑71 (MQ=255) tataGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGACGCATCAGGAACg > 1:483239/1‑71 (MQ=255) tataGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGACGCATCAGGAACg > 1:1592141/1‑71 (MQ=255) tataGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGACGCATCAGGAACg > 1:533497/1‑71 (MQ=255) tataGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGCACGCAACAgg > 1:1417736/1‑67 (MQ=255) aTGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGACGCATCAGGACCGGCAAttttt > 1:1293256/1‑70 (MQ=255) aTGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTc > 1:3912591/1‑71 (MQ=255) aTGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTc > 1:619771/1‑71 (MQ=255) tgCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCg < 1:412012/70‑1 (MQ=255) tgCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCg < 1:356644/70‑1 (MQ=255) tgCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCg < 1:2820897/70‑1 (MQ=255) tgCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCg < 1:3202700/70‑1 (MQ=255) tgCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCg < 1:1087348/70‑1 (MQ=255) ttGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTaa > 1:3954479/1‑71 (MQ=255) cagtccagtTCCATGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGAtt > 1:3951951/1‑71 (MQ=255) cagtccagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCg > 1:4362958/1‑67 (MQ=255) cagtccagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGAtt > 1:2818919/1‑71 (MQ=255) cagtccagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGAtt < 1:2899785/71‑1 (MQ=255) cagtccagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGAtt > 1:3566956/1‑71 (MQ=255) cagtccagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGAtt > 1:4746510/1‑71 (MQ=255) cagtccagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGAtt > 1:32672/1‑71 (MQ=255) cagtccagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGAtt > 1:1139762/1‑71 (MQ=255) cagtccagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGAtt > 1:845749/1‑71 (MQ=255) cagtccagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAG‑GGAttt > 1:3225839/1‑71 (MQ=255) tccagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTg < 1:3190011/71‑1 (MQ=255) tccagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTg < 1:805980/71‑1 (MQ=255) tccagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTg < 1:2171256/71‑1 (MQ=255) tccagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTg < 1:919376/71‑1 (MQ=255) tccagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTg < 1:2946236/71‑1 (MQ=255) tccagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTg < 1:1640246/71‑1 (MQ=255) tccagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTg < 1:1653265/71‑1 (MQ=255) ccagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTg < 1:3773811/70‑1 (MQ=255) cagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCCGCGGATTTTggg > 1:4256665/1‑71 (MQ=255) cagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGAtttt < 1:4716013/68‑1 (MQ=255) cagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGAtttt < 1:4437802/68‑1 (MQ=255) cagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGAtttt < 1:4431958/68‑1 (MQ=255) cagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGAtttt < 1:954317/68‑1 (MQ=255) cagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGAtttt < 1:1790154/68‑1 (MQ=255) cagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGAtttt < 1:1319566/68‑1 (MQ=255) cagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGAtttt < 1:1202272/68‑1 (MQ=255) cagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGAtttt < 1:3151425/68‑1 (MQ=255) cagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGAtttt < 1:3274238/68‑1 (MQ=255) cagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGAtttt < 1:89441/68‑1 (MQ=255) cagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGAtttt < 1:2974499/68‑1 (MQ=255) cagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGAtttt < 1:806512/68‑1 (MQ=255) cagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTggg > 1:2024135/1‑71 (MQ=255) cagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTggg > 1:3256489/1‑71 (MQ=255) ttCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGAtttt < 1:2275652/65‑1 (MQ=255) ttCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCa < 1:3046498/71‑1 (MQ=255) ttCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCa < 1:4651277/71‑1 (MQ=255) ttCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCa < 1:4594402/71‑1 (MQ=255) ttCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCa < 1:524919/71‑1 (MQ=255) ttCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCa < 1:1778374/71‑1 (MQ=255) ttCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCa < 1:247298/71‑1 (MQ=255) ttCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCa < 1:2409934/71‑1 (MQ=255) ttCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCa < 1:686953/71‑1 (MQ=255) ttCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCa < 1:3754457/71‑1 (MQ=255) ttCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCa < 1:2356485/71‑1 (MQ=255) tCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTg < 1:3475880/65‑1 (MQ=255) tCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCa < 1:4404684/70‑1 (MQ=255) ccACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAgg > 1:3053284/1‑71 (MQ=255) ccACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAgg > 1:1480595/1‑71 (MQ=255) ccACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAgg > 1:3066442/1‑71 (MQ=255) cACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGc < 1:4637685/71‑1 (MQ=255) cACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGc < 1:4037983/71‑1 (MQ=255) gCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTg < 1:3599683/60‑1 (MQ=255) ccGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCT‑CAGCGGATTTTGGGTCAGGCGACAATCGccc < 1:1832078/71‑1 (MQ=255) cGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGccc < 1:969392/71‑1 (MQ=255) cGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGccc < 1:3271558/71‑1 (MQ=255) cGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGccc < 1:3155874/71‑1 (MQ=255) cGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGccc < 1:2610174/71‑1 (MQ=255) cGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGccc < 1:2400201/71‑1 (MQ=255) cGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGccc < 1:1821701/71‑1 (MQ=255) cGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGccc < 1:1497951/71‑1 (MQ=255) cGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGccc < 1:1461261/71‑1 (MQ=255) | TTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGCCC > minE/1888987‑1889113 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |