Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	204,511	C→T	intergenic (+111/‑73)	alaV → / → rrlH	tRNA‑Ala/23S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	204,511	0	C	T	100.0%	47.1 / NA	17	intergenic (+111/‑73)	alaV/rrlH	tRNA‑Ala/23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (1/16);  total (1/16)

GAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGCAAGAAACATCTTCGGGT  >  minE/204497‑204578               |                                                                    gAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGaa              <  1:839955/70‑1 (MQ=255) gAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGaa              <  1:499243/70‑1 (MQ=255) gAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGaa              <  1:4770654/70‑1 (MQ=255) gAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGaa              <  1:4358461/70‑1 (MQ=255) gAAACACTGAACAATGAAAGTTGTTCGTGAGCCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGaa              <  1:4599594/70‑1 (MQ=11)  aaaGACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAAc            <  1:4651130/71‑1 (MQ=11)  aaaCACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGaa              <  1:2020081/69‑1 (MQ=255)  aaaCACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAAc            <  1:3895398/71‑1 (MQ=255)  aaaCACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAAc            <  1:604297/71‑1 (MQ=255)  aaaCACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAAc            <  1:4781840/71‑1 (MQ=255)  aaaCACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAAc            <  1:3252627/71‑1 (MQ=255)  aaaCACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAAc            <  1:2852114/71‑1 (MQ=255)  aaaCACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAAc            <  1:2710962/71‑1 (MQ=255)  aaaCACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAAc            <  1:2345633/71‑1 (MQ=255)  aaaCACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAAc            <  1:1750298/71‑1 (MQ=255)       cTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCtt       >  1:2919056/1‑71 (MQ=255)        tGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGaa              <  1:1235814/63‑1 (MQ=255)            caatGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc         >  1:793553/1‑64 (MQ=255)            caatGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCt        >  1:1356349/1‑65 (MQ=255)            caatGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGt  >  1:3509251/1‑71 (MQ=255)            caatGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGt  >  1:3885044/1‑71 (MQ=255)            caatGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGt  >  1:4093812/1‑71 (MQ=255)            caatGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGt  >  1:4140465/1‑71 (MQ=255)            caatGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGt  >  1:433303/1‑71 (MQ=255)            caatGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGt  >  1:2091433/1‑71 (MQ=255)            caatGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGt  >  1:1418037/1‑71 (MQ=255)               |                                                                    GAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGCAAGAAACATCTTCGGGT  >  minE/204497‑204578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑