Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,072,048	A→T	intergenic (‑88/‑131)	ygjH ← / → ebgR	conserved hypothetical protein/DNA‑binding transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,072,048	0	A	T	100.0%	125.7 / NA	40	intergenic (‑88/‑131)	ygjH/ebgR	conserved hypothetical protein/DNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (18/22);  total (18/22)

ACTAAACTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGATCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGAAACGCGCTTTATTTACTGAAAACAG  >  minE/2071987‑2072115                                                              |                                                                    aCTAAACTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTc                                                             <  1:3552605/70‑1 (MQ=255) aCTAAACTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTc                                                             <  1:2491014/70‑1 (MQ=255)  cTAAACTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTc                                                             <  1:2496575/69‑1 (MQ=255)     aaCTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTg                                                          <  1:1300930/69‑1 (MQ=255)     aaCTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTg                                                          <  1:4307412/69‑1 (MQ=255)     aaCTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTg                                                          <  1:3244019/69‑1 (MQ=255)     aaCTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTg                                                          <  1:2324012/69‑1 (MQ=255)            gCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTGAGCCGCtgt                                                 >  1:935919/1‑71 (MQ=255)            gCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTGAGCCGCtgt                                                 >  1:64614/1‑71 (MQ=255)            gCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTGAGCCGCtgt                                                 >  1:4559310/1‑71 (MQ=255)            gCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTGAGCCGCtgt                                                 >  1:3893052/1‑71 (MQ=255)            gCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTGAGCCGCtgt                                                 >  1:3127841/1‑71 (MQ=255)            gCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTGAGCCGCtgt                                                 >  1:1866477/1‑71 (MQ=255)            gCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTAGTGAGCCGCtg                                                  >  1:3369998/1‑70 (MQ=255)               aaaGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGt                                                           <  1:1080054/58‑1 (MQ=255)                 aGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTg                                                          <  1:2038042/57‑1 (MQ=255)                           aaTCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACa                                     <  1:2600359/68‑1 (MQ=255)                              ccTGTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTc                                >  1:2458464/1‑70 (MQ=255)                              ccTGTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTc                                >  1:1326891/1‑70 (MQ=255)                              ccTGTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTc                                >  1:588721/1‑70 (MQ=255)                                 gTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTc                                >  1:1575414/1‑67 (MQ=255)                                 gTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCa                               >  1:1562307/1‑68 (MQ=255)                                 gTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCa                               >  1:1418133/1‑68 (MQ=255)                                 gTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCa                               >  1:539397/1‑68 (MQ=255)                                 gTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCa                               >  1:2792233/1‑68 (MQ=255)                                 gTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCa                               >  1:2069703/1‑68 (MQ=255)                                 gTAGCCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCa                               >  1:3211696/1‑68 (MQ=255)                                  tAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGa                           <  1:705172/71‑1 (MQ=255)                                  tAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGa                           <  1:761056/71‑1 (MQ=255)                                  tAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGa                           <  1:3654755/71‑1 (MQ=255)                                  tAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGa                           <  1:3952181/71‑1 (MQ=255)                                  tAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGa                           <  1:2699424/71‑1 (MQ=255)                                    ggCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACa                                     <  1:4101120/59‑1 (MQ=255)                                       tAAAAATGGAGAATGCAGGCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGAAAcgc                      >  1:3046812/1‑71 (MQ=255)                                               gagaATGCAGGCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGAAACGCGCtttatt              <  1:1602914/71‑1 (MQ=255)                                               gagaATGCAGGCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGAAACGCGCtttatt              <  1:4570650/71‑1 (MQ=255)                                               gagaATGCAGGCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGAAACGCGCtttatt              <  1:260189/71‑1 (MQ=255)                                               gagaATGCAGGCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGAAACGCGCtttatt              <  1:749385/71‑1 (MQ=255)                                               gagaATGCAGGCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGAAACGCGCtttatt              <  1:249566/71‑1 (MQ=255)                                                agaATGCAGGCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGAAACGCGCtttattt             <  1:4557772/71‑1 (MQ=255)                                                           gtgtTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGAAACGCGCTTTATTTACTGAAAACAg  <  1:4578698/71‑1 (MQ=255)                                                           gtgtTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGAAACGCGCTTTATTTACTGAAAACAg  <  1:2989998/71‑1 (MQ=255)                                                           gtgtTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGAAACGCGCTTTATTTACTGAAAACAg  <  1:2386286/71‑1 (MQ=255)                                                              |                                                                    ACTAAACTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGATCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGAAACGCGCTTTATTTACTGAAAACAG  >  minE/2071987‑2072115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑