Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 2,402,822 T→C E70G (GAG→GGG)  yigA ← conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,402,8220TC100.0% 424.3 / NA 113E70G (GAG→GGG) yigAconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (74/39);  total (74/39)

Only 100 of 113 total aligned reads displayed.

GGCGATAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCTCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGACCAACGAAAC  >  minE/2402766‑2402880
                                                        |                                                          
ggCGATAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCa                                                      >  1:2281403/1‑63 (MQ=255)
ggCGATAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGAACATg                                               >  1:3902165/1‑70 (MQ=255)
ggCGATAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGAACATg                                               >  1:2038909/1‑70 (MQ=255)
ggCGATAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGAACATg                                               <  1:207017/70‑1 (MQ=255)
ggCGATAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGAACATg                                               >  1:2306010/1‑70 (MQ=255)
ggCGATAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGAACATg                                               <  1:253291/70‑1 (MQ=255)
ggCGATAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGAACATg                                               >  1:2591869/1‑70 (MQ=255)
ggCGATAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGAACATg                                               >  1:3003409/1‑70 (MQ=255)
ggCGATAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGAACATg                                               <  1:3858058/70‑1 (MQ=255)
ggCGATAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGAACATg                                               >  1:1096695/1‑70 (MQ=255)
ggCGATAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGAACATg                                               <  1:3987616/70‑1 (MQ=255)
ggCGATAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGAACATg                                               <  1:4320836/70‑1 (MQ=255)
ggCGATAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGAACATg                                               >  1:449323/1‑70 (MQ=255)
ggCGATAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGAACATg                                               <  1:4619150/70‑1 (MQ=255)
ggCGATAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGAACATg                                               <  1:4752689/70‑1 (MQ=255)
ggCGATAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGAACATg                                               <  1:604102/70‑1 (MQ=255)
ggCGATAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGAACATg                                               <  1:700590/70‑1 (MQ=255)
ggCGATAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGAACATg                                               <  1:859143/70‑1 (MQ=255)
ggCGATAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGAACATg                                               >  1:1151307/1‑70 (MQ=255)
ggCGATAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCAAGAACATg                                               >  1:4768221/1‑70 (MQ=255)
   gATAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGAACATGAata                                           >  1:3761835/1‑71 (MQ=255)
   gATAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGAACATGAata                                           >  1:3513757/1‑71 (MQ=255)
   gATAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGAACATGAata                                           >  1:3329321/1‑71 (MQ=255)
   gATAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGAACATGAata                                           >  1:558328/1‑71 (MQ=255)
   gATAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGAACATGAata                                           >  1:574834/1‑71 (MQ=255)
   gATAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGAACATGAata                                           >  1:2824907/1‑71 (MQ=255)
   gATAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGAACATGAata                                           >  1:2724202/1‑71 (MQ=255)
     tAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGaa                                                   <  1:3254113/61‑1 (MQ=255)
     tAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGaa                                                   <  1:4583420/61‑1 (MQ=255)
     tAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGaa                                                   <  1:1208414/61‑1 (MQ=255)
     tAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGaa                                                   <  1:4010621/61‑1 (MQ=255)
     tAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGaa                                                   <  1:4737371/61‑1 (MQ=255)
     tAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGaa                                                   <  1:2962324/61‑1 (MQ=255)
     tAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGaa                                                   <  1:2844478/61‑1 (MQ=255)
     tAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGaa                                                   <  1:858996/61‑1 (MQ=255)
     tAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGaa                                                   <  1:2638062/61‑1 (MQ=255)
     tAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGAACATGAatat                                          >  1:1131239/1‑70 (MQ=255)
     tAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGAACATGAatat                                          >  1:520068/1‑70 (MQ=255)
     tAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGAACATGAatat                                          >  1:669184/1‑70 (MQ=255)
     tAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGAACATGAatat                                          >  1:3454399/1‑70 (MQ=255)
     tAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGAACATGAatat                                          >  1:3309087/1‑70 (MQ=255)
     tAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGAACATGAatat                                          >  1:2240066/1‑70 (MQ=255)
     tAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGAACATGAatat                                          >  1:531193/1‑70 (MQ=255)
     tAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGAACATGAatat                                          >  1:1102540/1‑70 (MQ=255)
     tAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGAACATGAatat                                          >  1:4641170/1‑70 (MQ=255)
     tAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGAACATGAatat                                          >  1:1714259/1‑70 (MQ=255)
     tAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGAACATGAatat                                          >  1:402771/1‑70 (MQ=255)
         aCAGGCC‑TCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGAACATGAATATGATTAc                                    <  1:2846542/71‑1 (MQ=255)
          cAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGAACATGAATATGATTAc                                    <  1:3177874/71‑1 (MQ=255)
            ggCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGAACATGAATATGATTACg                                   >  1:4475777/1‑70 (MQ=255)
                ttCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGAACATGaa                                             <  1:4211261/56‑1 (MQ=255)
                ttCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGAACATGaa                                             <  1:3945674/56‑1 (MQ=255)
                ttCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGAACATGaa                                             <  1:332309/56‑1 (MQ=255)
                ttCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGAACATGaa                                             <  1:3205058/56‑1 (MQ=255)
                ttCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGAACATGaa                                             <  1:1540005/56‑1 (MQ=255)
                ttCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGAACATGaa                                             <  1:1517914/56‑1 (MQ=255)
                ttCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGAACATGaa                                             <  1:1974565/56‑1 (MQ=255)
                 tCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGAACATGAATATGATTACGTgcgc                              >  1:42173/1‑70 (MQ=255)
                 tCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGAACATGAATATGATTACGTGcgcg                             >  1:4454729/1‑71 (MQ=255)
                 tCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGAACATGAATATGATTACGTGcgcg                             >  1:2192350/1‑71 (MQ=255)
                 tCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGAACATGAATATGATTACGTGcgcg                             >  1:1373062/1‑71 (MQ=255)
                 tCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGAACATGAATATGATTACGTGcgcg                             >  1:4690029/1‑71 (MQ=255)
                 tCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGAACATGAATATGATTACGTGcgcg                             >  1:178954/1‑71 (MQ=255)
                      ggCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGAACATGAATATGATTACGTGCGCGGGCCa                        >  1:266253/1‑71 (MQ=255)
                              ccTGTTCCATCAACAGCGCCATGTTCCCTTCCAGaa                                                   <  1:3120183/36‑1 (MQ=255)
                                  ttCCATCAACAGCGCCATGTTCCCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTc              <  1:4633899/69‑1 (MQ=255)
                                  ttCCATCAACAGCGCCATGTTCCCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTc              <  1:3707962/69‑1 (MQ=255)
                                  ttCCATCAACAGCGCCATGTTCCCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTc              <  1:4681987/69‑1 (MQ=255)
                                  ttCCATCAACAGCGCCATGTTCCCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTc              <  1:1379813/69‑1 (MQ=255)
                                  ttCCATCAACAGCGCCATGTTCCCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTc              <  1:560000/69‑1 (MQ=255)
                                  ttCCATCAACAGCGCCATGTTCCCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTc              <  1:2650739/69‑1 (MQ=255)
                                  ttCCATCAACAGCGCCATGTTCCCTTCCAGAACATGAATATGATTACGTGAGCGGGCCATGTGCCACTc              <  1:156959/69‑1 (MQ=255)
                                    ccATCAACAGCGCCATGTTCCCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGAc           >  1:4712346/1‑70 (MQ=255)
                                    ccATCAACAGCGCCATGTTCCCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGAc           >  1:1087706/1‑70 (MQ=255)
                                    ccATCAACAGCGCCATGTTCCCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGAc           >  1:564250/1‑70 (MQ=255)
                                    ccATCAACAGCGCCATGTTCCCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGAc           >  1:562131/1‑70 (MQ=255)
                                    ccATCAACAGCGCCATGTTCCCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGAc           >  1:4053187/1‑70 (MQ=255)
                                    ccATCAACAGCGCCATGTTCCCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGAc           >  1:1706770/1‑70 (MQ=255)
                                    ccATCAACAGCGCCATGTTCCCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGAc           >  1:1391226/1‑70 (MQ=255)
                                    ccATCAACAGCGCCATGTTCCCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGAc           >  1:3765509/1‑70 (MQ=255)
                                    ccATCAACAGCGCCATGTTCCCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGAc           >  1:2286316/1‑70 (MQ=255)
                                    ccATCAACAGCGCCATGTTCCCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGAc           >  1:3682635/1‑70 (MQ=255)
                                    ccATCAACAGCGCCATGTTCCCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGAc           >  1:3424429/1‑70 (MQ=255)
                                    ccATCAACAGCGCCATGTTCCCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGAc           >  1:269383/1‑70 (MQ=255)
                                     cATCAACAGCGCCATGTTCCCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGAcc          >  1:2733154/1‑70 (MQ=255)
                                     cATCAACAGCGCCATGTTCCCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGAcc          >  1:1731750/1‑70 (MQ=255)
                                     cATCAACAGCGCCATGTTCCCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGAcc          >  1:835362/1‑70 (MQ=255)
                                     cATCAACAGCGCCATGTTCCCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGAcc          >  1:1123029/1‑70 (MQ=255)
                                     cATCAACAGCGCCATGTTCCCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGAcc          >  1:2443107/1‑70 (MQ=255)
                                     cATCAACAGCGCCATGTTCCCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGAcc          >  1:145820/1‑70 (MQ=255)
                                     cATCAACAGCGCCATGTTCCCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGAcc          >  1:1414800/1‑70 (MQ=255)
                                     cATCAACAGCGCCATGTTCCCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGAcc          >  1:373899/1‑70 (MQ=255)
                                     cATCAACAGCGCCATGTTCCCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGAcc          >  1:4684870/1‑70 (MQ=255)
                                     cATCAACAGCGCCATGTTCCCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGAcc          >  1:1330766/1‑70 (MQ=255)
                                     cATCAACAGCGCCATGTTCCCTTCCAGAACATCAATATGATTACGTGCGCGGGCCATGAGCCACTCGAcc          >  1:4015079/1‑70 (MQ=255)
                                         aaCAGCGCCATGTTCCCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGACCAAc       >  1:708082/1‑69 (MQ=255)
                                         aaCAGCGCCATGTTCCCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGACCAACg      >  1:3185741/1‑70 (MQ=255)
                                            aGCGCCATGTTCCCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGACCAACGAAAc  >  1:4494651/1‑71 (MQ=255)
                                            aGCGCCATGTTCCCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGACCAACGAAAc  >  1:677074/1‑71 (MQ=255)
                                            aGCGCCATGTTCCCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGACCAACGAAAc  >  1:1790078/1‑71 (MQ=255)
                                                        |                                                          
GGCGATAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCTCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGACCAACGAAAC  >  minE/2402766‑2402880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑