Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,421,748	T→C	K196K (AAA→AAG)	wzxE ←	O‑antigen translocase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,421,748	0	T	C	100.0%	287.0 / NA	77	K196K (AAA→AAG)	wzxE	O‑antigen translocase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/62);  total (15/62)

ACTGCCCTGCCAGACCGTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGTTTGATCAACATGATGGCGGCAGGAATTACCACCAGCGCGGGAATCAGCGCCAGACCCAGCAACGCC  >  minE/2421687‑2421814                                                              |                                                                   aCTGCCCTGCCAGACCGTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGATCAAc                                                           <  1:4926845/71‑1 (MQ=255) aCTGCCCTGCCAGACCGTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGATCAAc                                                           <  1:2475376/71‑1 (MQ=255) aCTGCCCTGCCAGACCGTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGATCAAc                                                           <  1:4884328/71‑1 (MQ=255) aCTGCCCTGCCAGACCGTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGATCAAc                                                           <  1:3946204/71‑1 (MQ=255) aCTGCCCTGCCAGACCGTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGATCAAc                                                           <  1:1330715/71‑1 (MQ=255) aCTGCCCTGCCAGACCGTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGATCAAc                                                           <  1:657348/71‑1 (MQ=255) aCTGCCCTGCCAGACCGTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGATCAAc                                                           <  1:850977/71‑1 (MQ=255)  ctgccctgccAGACCGTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGATCAACa                                                          <  1:3440594/71‑1 (MQ=255)  ctgccctgccAGACCGTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGATCAACa                                                          <  1:2846205/71‑1 (MQ=255)  ctgccctgccAGACCGTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGATCAACa                                                          <  1:4834676/71‑1 (MQ=255)     ccctgccAGACCGTTATCCCAGCTTGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGATCAACat                                                         <  1:4715689/69‑1 (MQ=255)     ccctgccAGACCGTTATCCCAGCTTGGTTTCAGATAGATTAACGGGATGACACCACGCTTTATCAACat                                                         <  1:478683/69‑1 (MQ=255)     ccctgccAGACCGTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGATCAACat                                                         <  1:3734888/69‑1 (MQ=255)     ccctgccAGACCGTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGATCAACat                                                         <  1:4297567/69‑1 (MQ=255)     ccctgccAGACCGTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGATCAACat                                                         <  1:2522902/69‑1 (MQ=255)     ccctgccAGACCGTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGATCAACat                                                         <  1:2874155/69‑1 (MQ=255)     ccctgccAGACCGTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGATCAACat                                                         <  1:4569168/69‑1 (MQ=255)     ccctgccAGACCGTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGATCAACat                                                         <  1:2482738/69‑1 (MQ=255)     ccctgccAGACCGTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGATCAACat                                                         <  1:4813321/69‑1 (MQ=255)     ccctgccAGACCGTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGATCAACat                                                         <  1:1386971/69‑1 (MQ=255)     ccctgccAGACCGTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGATCAACat                                                         <  1:1418403/69‑1 (MQ=255)     ccctgccAGACCGTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGATCAACat                                                         <  1:2231077/69‑1 (MQ=255)     ccctgccAGACCGTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGATCAACat                                                         <  1:2071398/69‑1 (MQ=255)         gccAGACCGTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGATCAACATGATggc                                                   >  1:2858107/1‑71 (MQ=255)               ccGTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGATCAACATGATGGCGGCAgg                                             <  1:2482772/71‑1 (MQ=255)               ccGTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGATCAACATGATGGCGGCAgg                                             <  1:3536560/71‑1 (MQ=255)               ccGTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGATCAACATGATGGCGGCAgg                                             <  1:3166401/71‑1 (MQ=255)               ccGTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGATCAACATGATGGCGGCAgg                                             <  1:42786/71‑1 (MQ=255)               ccGTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGATCAACATGATGGCGGCAgg                                             <  1:3049255/71‑1 (MQ=255)               ccGTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGATCAACATGATGGCGGCAgg                                             <  1:2919831/71‑1 (MQ=255)               ccGTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGATCAACATGATGGCGGCAgg                                             <  1:2630435/71‑1 (MQ=255)               ccGTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGATCAACATGATGGCGGCAgg                                             <  1:4854476/71‑1 (MQ=255)                cGTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGATCAACATGATGGCGGCAgg                                             <  1:4256029/70‑1 (MQ=255)                 gTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGa                                                                <  1:56255/50‑1 (MQ=255)                 gTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGa                                                                <  1:4304359/50‑1 (MQ=255)                 gTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGa                                                                <  1:4523568/50‑1 (MQ=255)                 gTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGa                                                                <  1:4209426/50‑1 (MQ=255)                 gTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGa                                                                <  1:3995910/50‑1 (MQ=255)                 gTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGa                                                                <  1:638857/50‑1 (MQ=255)                 gTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGa                                                                <  1:3522876/50‑1 (MQ=255)                 gTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGa                                                                <  1:3646432/50‑1 (MQ=255)                 gTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGa                                                                <  1:2319686/50‑1 (MQ=255)                 gTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGa                                                                <  1:2879182/50‑1 (MQ=255)                 gTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGa                                                                <  1:3069924/50‑1 (MQ=255)                 gTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGa                                                                <  1:2718200/50‑1 (MQ=255)                 gTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGa                                                                <  1:2287943/50‑1 (MQ=255)                  ttATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGa                                                                <  1:4694320/49‑1 (MQ=255)                    aTCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGATCAACATGATGGCGGCAGGAAtt                                         <  1:3767860/70‑1 (MQ=255)                    aTCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGATCAACATGATGGCGGCAGGAAtt                                         <  1:2394812/70‑1 (MQ=255)                    aTCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGATCAACATGATGGCGGCAGGAAtt                                         <  1:3071542/70‑1 (MQ=255)                    aTCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGATCAACATGATGGCGGCAGGAAtt                                         <  1:1226503/70‑1 (MQ=255)                      cccAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGATCAACatga                                                       <  1:2055259/54‑1 (MQ=255)                      cccAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGATCAACatga                                                       <  1:3836817/54‑1 (MQ=255)                      cccAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGATCAACatga                                                       <  1:3360057/54‑1 (MQ=255)                      cccAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGATCAACatga                                                       <  1:1673788/54‑1 (MQ=255)                      cccAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGATCAACatga                                                       <  1:4469053/54‑1 (MQ=255)                      cccAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGATCAACatga                                                       <  1:1210489/54‑1 (MQ=255)                       ccAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGATCAACatga                                                       <  1:4316638/53‑1 (MQ=255)                       ccAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGATCAACatga                                                       <  1:3660954/53‑1 (MQ=255)                        cAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGa                                                                <  1:3564318/43‑1 (MQ=255)                        cAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGCTTGATCAACATGATGGCGGCAGGAATTaccac                                    >  1:1115553/1‑71 (MQ=255)                                  tCAGATAGCTTAACGGGATGACACCACGCTTGATCAACATGATGGCGGCAGGAATTACCACCAGCGCggg                           >  1:4844592/1‑70 (MQ=255)                                  tCAGATAGCTTAACGGGATGACACCACGCTTGATCAACATGATGGCGGCAGGAATTACCACCAGCGCggg                           >  1:2508280/1‑70 (MQ=255)                                    aGATAGCTTAACGGGATGACACCACGCTTGATCAACATGATGGCGGCAGGAATTAGCACCAGCGCGGGAAt                        >  1:4266284/1‑71 (MQ=255)                                    aGAAAGCTTAACGGGATGACACCACGCTTGATCAACATGATGGCGGCAGGAATTACCACCAg                                 >  1:2811370/1‑62 (MQ=255)                                          cTTAACGGGATGACACCACGCTTGATCAACATGATGGCGGCAGGAATTACCACCAGCGCGGGAATCAgcgc                  <  1:3493061/71‑1 (MQ=255)                                            tAACGGGATGACACCACGCTTGATCAACATGATGGCGGCAGGAATTACCACCAGCGCGGGAATCAgcgc                  >  1:3927818/1‑69 (MQ=255)                                                  gATGACACGACGCTTGATCAACATGATGGCGGCAgg                                             <  1:15293/36‑1 (MQ=37)                                                         ccacGCTTGATCAACATGATGGCGGCAGGAATTACCACCAGCGCGGGa                          <  1:1916060/48‑1 (MQ=255)                                                          cacGCTTGATCAACATGATGGCGGCAGGAATTACCACCAGCGCGGGAATCAGCGCCAGACCCAGCAACGcc  >  1:4652803/1‑71 (MQ=255)                                                          cacGCTTGATCAACATGATGGCGGCAGGAATTACCACCAGCGCGGGAATCAGCGCCAGACCCAGCAACGcc  >  1:2529263/1‑71 (MQ=255)                                                          cacGCTTGATCAACATGATGGCGGCAGGAATTACCACCAGCGCGGGAATCAGCGCCAGACCCAGCAACGcc  >  1:3690425/1‑71 (MQ=255)                                                          cacGCTTGATCAACATGATGGCGGCAGGAATTACCACCAGCGCGGGAATCAGCGCCAGACCCAGCAACGcc  >  1:3671867/1‑71 (MQ=255)                                                          cacGCTTGATCAACATGATGGCGGCAGGAATTACCACCAGCGCGGGAATCAGCGCCAGACCCAGCAACGcc  >  1:3321878/1‑71 (MQ=255)                                                          cacGCTTGATCAACATGATGGCGGCAGGAATTACCACCAGCGCGGGAATCAGCGCCAGACCCAGCAACGc   >  1:3552478/1‑70 (MQ=255)                                                          cacGCTTGATCAACATGATGGCGGCAGGAATTACCACCAGCGCAGGAATCAGCGCCAGACCCAGCAACGcc  >  1:2317840/1‑71 (MQ=255)                                                          cacGCTTGATCAACATGATGGCGGCAGGAATTACAACACGCGCGGGAATCAg                     >  1:3976859/1‑52 (MQ=255)                                                              |                                                                   ACTGCCCTGCCAGACCGTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGTTTGATCAACATGATGGCGGCAGGAATTACCACCAGCGCGGGAATCAGCGCCAGACCCAGCAACGCC  >  minE/2421687‑2421814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑