BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,748,276	A→G	S117G (AGC→GGC)	secY →	preprotein translocase membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,748,276	0	A	G	100.0%	415.2 / NA	110	S117G (AGC→GGC)	secY	preprotein translocase membrane subunit
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (65/45); total (65/45)

Only 100 of 110 total aligned reads displayed.

CTGACGGTGGTTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCAGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGTATTGCTACCGGT  >  minE/2748210‑2748341

ctgACGGTGGTTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGc                                                                 >  1:126769/1‑69 (MQ=255)

ctgACGGTGGTTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGc                                                                 >  1:61971/1‑69 (MQ=255)

ctgACGGTGGTTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGc                                                                 >  1:523627/1‑69 (MQ=255)

ctgACGGTGGTTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGc                                                                 >  1:78453/1‑69 (MQ=255)

ctgACGGTGGTTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGc                                                                 >  1:3756390/1‑69 (MQ=255)

ctgACGGTGGTTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGc                                                                 >  1:1799852/1‑69 (MQ=255)

ctgACGGTGGTTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGc                                                                 >  1:4911113/1‑69 (MQ=255)

ctgACGGTGGTTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGc                                                                 >  1:190017/1‑69 (MQ=255)

ctgACGGTGGTTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGc                                                                 >  1:4184726/1‑69 (MQ=255)

ctgACGGTGGTTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGc                                                                 >  1:4224328/1‑69 (MQ=255)

ctgACGGTGGTTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGc                                                                 >  1:3389495/1‑69 (MQ=255)

ctgACGGTGGTTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGc                                                                 >  1:2025188/1‑69 (MQ=255)

ctgACGGTGGTTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGc                                                                 >  1:3173725/1‑69 (MQ=255)

ctgACGGTGGTTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGc                                                                 >  1:2552/1‑69 (MQ=255)

ctgACGGTGGTTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGc                                                                 >  1:1135605/1‑69 (MQ=255)

ctgACGGTGGTTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGc                                                                 >  1:3265440/1‑69 (MQ=255)

      gtggtTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGt                                                             <  1:2662765/67‑1 (MQ=255)

      gtggtTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGt                                                             <  1:3886847/67‑1 (MQ=255)

      gtggtTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTa                                                            >  1:722146/1‑68 (MQ=255)

      gtggtTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTa                                                            >  1:520466/1‑68 (MQ=255)

      gtggtTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTa                                                            >  1:4445513/1‑68 (MQ=255)

      gtggtTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTa                                                            >  1:1026271/1‑68 (MQ=255)

      gtggtTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTa                                                            >  1:2343133/1‑68 (MQ=255)

      gtggtTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTa                                                            >  1:442935/1‑68 (MQ=255)

      gtggtTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTa                                                            >  1:3386493/1‑68 (MQ=255)

      gtggtTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTa                                                            >  1:4360636/1‑68 (MQ=255)

      gtggtTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTa                                                            >  1:798152/1‑68 (MQ=255)

      gtggtTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTa                                                            >  1:3637031/1‑68 (MQ=255)

      gtggtTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTa                                                            >  1:1349568/1‑68 (MQ=255)

      gtggtTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTa                                                            >  1:4931720/1‑68 (MQ=255)

      gtggtTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTa                                                            >  1:2932303/1‑68 (MQ=255)

      gtggtTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTa                                                            >  1:4143940/1‑68 (MQ=255)

          ttCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGc                                                     <  1:2136345/71‑1 (MQ=255)

           tCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTa                                                            >  1:3076567/1‑63 (MQ=255)

           tCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTa                                                            >  1:4531575/1‑63 (MQ=255)

           tCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTa                                                            >  1:2630988/1‑63 (MQ=255)

           tCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTa                                                            >  1:3855675/1‑63 (MQ=255)

           tCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTa                                                            >  1:141436/1‑63 (MQ=255)

           tCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGc                                                     >  1:3653101/1‑70 (MQ=255)

             aCCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACAcc                                                        <  1:2702766/65‑1 (MQ=255)

             aCCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACAcc                                                        <  1:2199973/65‑1 (MQ=255)

               ccAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACgg                                                <  1:3284255/71‑1 (MQ=255)

               ccAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACgg                                                <  1:3808910/71‑1 (MQ=255)

               ccAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACgg                                                <  1:1055005/71‑1 (MQ=255)

               ccAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACgg                                                <  1:1811152/71‑1 (MQ=255)

               ccAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACgg                                                <  1:2929993/71‑1 (MQ=255)

               ccAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACgg                                                <  1:1254972/71‑1 (MQ=255)

                cAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACgg                                                >  1:552150/1‑70 (MQ=255)

                       ggCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACGGTACTCTg                                         <  1:1827429/70‑1 (MQ=255)

                        gCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGt                                       >  1:3133558/1‑71 (MQ=255)

                            aaaTTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGc                                                     <  1:4542814/53‑1 (MQ=255)

                                 aagaaagaAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCaa                               >  1:3309463/1‑70 (MQ=255)

                                 aagaaagaAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCaa                               >  1:3621761/1‑70 (MQ=255)

                                 aagaaagaAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCaa                               >  1:4138696/1‑70 (MQ=255)

                                     aagaagGGGAGTCTGGTCGTCGTAAGATCGGCCAGt                                                             <  1:2696506/36‑1 (MQ=255)

                                     aagaagGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACgg                                                <  1:241978/49‑1 (MQ=255)

                                        aagGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCa                        >  1:2041300/1‑70 (MQ=255)

                                        aagGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCa                        >  1:1948171/1‑70 (MQ=255)

                                        aagGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAg                       >  1:117311/1‑71 (MQ=255)

                                        aagGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAg                       >  1:4492028/1‑71 (MQ=255)

                                        aagGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAg                       >  1:4067503/1‑71 (MQ=255)

                                         cggggAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGc                                                     <  1:4400888/39‑1 (MQ=39)

                                         agGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACAcc                                                        <  1:1294585/37‑1 (MQ=255)

                                         agGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACgg                                                <  1:2552773/45‑1 (MQ=255)

                                         agGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACgg                                                <  1:3921813/45‑1 (MQ=255)

                                         agGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACgg                                                <  1:4041289/45‑1 (MQ=255)

                                          ggggAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCaa                               <  1:4695076/61‑1 (MQ=255)

                                          ggggAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCaa                               <  1:1160180/61‑1 (MQ=255)

                                          ggggAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCaa                               <  1:3412085/61‑1 (MQ=255)

                                          ggggAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCaa                               <  1:1737111/61‑1 (MQ=255)

                                          ggggAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCaa                               <  1:3358692/61‑1 (MQ=255)

                                          ggggAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCaa                               <  1:3037217/61‑1 (MQ=255)

                                          ggggAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCaa                               <  1:1467079/61‑1 (MQ=255)

                                           gggAGTCTGGTCGTCGTAAGATCGGCCAGTACAcc                                                        <  1:2996784/35‑1 (MQ=255)

                                           gggAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGc                                                     <  1:4582731/38‑1 (MQ=255)

                                           gggAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCaa                               <  1:165551/60‑1 (MQ=255)

                                           gggAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCaa                               <  1:290056/60‑1 (MQ=255)

                                           gggAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCg                    <  1:2802155/71‑1 (MQ=255)

                                               gTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCaa                               <  1:1758562/56‑1 (MQ=255)

                                                    gtcgtcGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGt              <  1:1557895/68‑1 (MQ=255)

                                                    gtcgtcGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGt              <  1:4612469/68‑1 (MQ=255)

                                                    gtcgtcGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGt              <  1:1149353/68‑1 (MQ=255)

                                                    gtcgtcGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGt              <  1:336922/68‑1 (MQ=255)

                                                    gtcgtcGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGt              <  1:451662/68‑1 (MQ=255)

                                                    gtcgtcGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGTAtt           >  1:3245491/1‑71 (MQ=255)

                                                    gtcgtcGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGTAtt           >  1:3803410/1‑71 (MQ=255)

                                                    gtcgtcGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGTAtt           >  1:1174010/1‑71 (MQ=255)

                                                    gtcgtcGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGTAt            >  1:4597274/1‑70 (MQ=255)

                                                       gtcgtAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGt              <  1:1602888/65‑1 (MQ=255)

                                                          gtAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGTATTGCTAcc     >  1:4544649/1‑71 (MQ=255)

                                                          gtAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGTATTGCTAc      <  1:947591/70‑1 (MQ=255)

                                                            aaGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGTATTGCTACCgg   >  1:3706075/1‑71 (MQ=255)

                                                            aaGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGTATTGCTACCgg   >  1:3110816/1‑71 (MQ=255)

                                                            aaGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGTATTGCTACCgg   >  1:2912482/1‑71 (MQ=255)

                                                            aaGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGTATTGCTACCgg   >  1:1955178/1‑71 (MQ=255)

                                                            aaGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGTATTGCTACCgg   >  1:1655907/1‑71 (MQ=255)

                                                            aaGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGTATTGCTACCgg   >  1:157552/1‑71 (MQ=255)

                                                             aGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGTATTGCTACCgg   >  1:2972308/1‑70 (MQ=255)

                                                             aGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGTATTGCTACCGGt  >  1:4579966/1‑71 (MQ=255)

                                                             aGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGTATTGCTACCGGt  >  1:3736232/1‑71 (MQ=255)

CTGACGGTGGTTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCAGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGTATTGCTACCGGT  >  minE/2748210‑2748341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`