BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	244,476	A→G	Y316H (TAT→CAT)	sbcD ←	exonuclease, dsDNA, ATP‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	244,476	0	A	G	100.0%	436.2 / NA	115	Y316H (TAT→CAT)	sbcD	exonuclease, dsDNA, ATP‑dependent
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (83/32); total (83/32)

Only 100 of 115 total aligned reads displayed.

GCAATACTTCGACAGGCAATGATTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATACTCATCAGTAGTGATTTCGATATCCAGCCAGACAGGTGGCTCCTGCGATACATCGCGCCACT  >  minE/244413‑244538

gCAATACTTCGACAGGCAATGATTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATGCtcat                                                           >  1:4210244/1‑69 (MQ=255)

gCAATACTTCGACAGGCAATGATTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATGCtcat                                                           >  1:2878106/1‑69 (MQ=255)

    tACTTCGACAGGCAATGATTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATGCTCATCagtag                                                     >  1:2887568/1‑71 (MQ=255)

    tACTTCGACAGGCAATGATTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATGCTCATCagtag                                                     >  1:1962345/1‑71 (MQ=255)

    tACTTCGACAGGCAATGATTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATGCTCATCagtag                                                     >  1:3357670/1‑71 (MQ=255)

    tACTTCGACAGGCAATGATTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATGCTCATCagtag                                                     >  1:2071079/1‑71 (MQ=255)

    tACTTCGACAGGCAATGATTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATGCTCATCagtag                                                     >  1:4139703/1‑71 (MQ=255)

    tACTTCGACAGGCAATGATTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATGCTCATCagtag                                                     >  1:2381310/1‑71 (MQ=255)

    tACTTCGACAGGCAATGATTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATGCTCATCagtag                                                     >  1:2519659/1‑71 (MQ=255)

    tACTTCGACAGGCAATGATTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATGCTCATCagtag                                                     >  1:932043/1‑71 (MQ=255)

      cTTCGACAGGCAATGATTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATGCt                                                              >  1:3878742/1‑60 (MQ=255)

      cTTCGACAGGCAATGATTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATGCTCATCagtagt                                                    >  1:3608847/1‑70 (MQ=255)

                 aaTGATTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGATTTCGatata                                        <  1:3232346/71‑2 (MQ=255)

                  aTGATTCGGATAATGCCTGGATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGATTTCGATATcc                                       <  1:730580/71‑1 (MQ=255)

                     aTTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGa                                                  >  1:2770748/1‑57 (MQ=255)

                     aTTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGa                                                  >  1:2505841/1‑57 (MQ=255)

                     aTTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGa                                                  >  1:2701282/1‑57 (MQ=255)

                     aTTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGa                                                  >  1:2151911/1‑57 (MQ=255)

                     aTTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGa                                                  >  1:2725378/1‑57 (MQ=255)

                     aTTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGa                                                  >  1:2053017/1‑57 (MQ=255)

                     aTTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGa                                                  >  1:3189906/1‑57 (MQ=255)

                     aTTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGa                                                  >  1:2004842/1‑57 (MQ=255)

                     aTTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGa                                                  >  1:3523861/1‑57 (MQ=255)

                     aTTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGa                                                  >  1:3651846/1‑57 (MQ=255)

                     aTTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGa                                                  >  1:3678984/1‑57 (MQ=255)

                     aTTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGa                                                  >  1:1694356/1‑57 (MQ=255)

                     aTTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGa                                                  >  1:4165331/1‑57 (MQ=255)

                     aTTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGa                                                  >  1:4454423/1‑57 (MQ=255)

                     aTTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGa                                                  >  1:452511/1‑57 (MQ=255)

                     aTTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGa                                                  >  1:632073/1‑57 (MQ=255)

                        cGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGATTTCGATATccagcca                                  <  1:445182/70‑1 (MQ=255)

                        cGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGATTTCGATATccagc                                    >  1:3377734/1‑68 (MQ=255)

                        cGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGATTTCGATATccagc                                    >  1:4902890/1‑68 (MQ=255)

                        cGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGATTTCGATATccagc                                    >  1:728174/1‑68 (MQ=255)

                        cGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGATTTCGATATccagc                                    >  1:3294106/1‑68 (MQ=255)

                        cGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGATTTCGATATccagc                                    >  1:1487093/1‑68 (MQ=255)

                        cGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGATTTCGATATccagc                                    >  1:1902936/1‑68 (MQ=255)

                        cGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGATTTCGATATccagc                                    >  1:2026633/1‑68 (MQ=255)

                            tAATGCCTGGATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGATTTCGATATCCAGCCAGACAg                             >  1:252781/1‑71 (MQ=255)

                            tAATGCCTGGATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGATTTCGATATCCAGCCAGACAg                             >  1:4353620/1‑71 (MQ=255)

                            tAATGCCTGGATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGATTTCGATATCCAGCCAGACAg                             >  1:3877595/1‑71 (MQ=255)

                            tAATGCCTGGATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGATTTCGATATCCAGCCAGACAg                             >  1:701965/1‑71 (MQ=255)

                            tAATGCCTGGATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGATTTCGATATCCAGCCAGACAg                             >  1:4585574/1‑71 (MQ=255)

                             aaTGCCTGGATTTTGCGCTGTATATCATGCAGATGCTCATCAGTAGTGATTTCGATATCCAGCCAGACa                              >  1:1212887/1‑69 (MQ=255)

                             aaTGCCTGGATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGATTTCGATATCCAGCCAGACa                              >  1:3385541/1‑69 (MQ=255)

                             aaTGCCTGGATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGATTTCGATATCCAGCCAGACa                              >  1:3677332/1‑69 (MQ=255)

                             aaTGCCTGGATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGATTTCGATATCCAGCCAGACa                              >  1:1180648/1‑69 (MQ=255)

                             aaTGCCTGGATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGATTTCGATATCCAGCCAGACa                              >  1:186984/1‑69 (MQ=255)

                             aaTGCCTGGATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGATTTCGATATCCAGCCAGACa                              >  1:3196186/1‑69 (MQ=255)

                             aaTGCCTGGATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGATTTCGATATCCAGCCAGACa                              >  1:3122289/1‑69 (MQ=255)

                                 ccTGGATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGATTTCGATATCCAGCCAGACAGGTgg                         >  1:4526118/1‑70 (MQ=255)

                                 ccTGGATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGATTTCGATATCCAGCCAGACAGGTgg                         >  1:4590408/1‑70 (MQ=255)

                                 ccTGGATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGATTTCGATATCCAGCCAGACAGGTGGc                        >  1:1107524/1‑71 (MQ=255)

                                 ccTGGATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGATTTCGATATCCAGCCAGACAGGTGGc                        >  1:820551/1‑71 (MQ=255)

                                 ccTGGATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGATTTCGATATCCAGCCAGACAGGTGGc                        >  1:4707046/1‑71 (MQ=255)

                                     gATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGAtt                                                >  1:485885/1‑43 (MQ=255)

                                     gATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGAtt                                                >  1:4408858/1‑43 (MQ=255)

                                     gATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGAtt                                                >  1:4596924/1‑43 (MQ=255)

                                     gATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGAtt                                                >  1:368154/1‑43 (MQ=255)

                                     gATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGAtt                                                >  1:1332120/1‑43 (MQ=255)

                                     gATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGAtt                                                >  1:3055537/1‑43 (MQ=255)

                                     gATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGAtt                                                >  1:2339934/1‑43 (MQ=255)

                                     gATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGAtt                                                >  1:1473124/1‑43 (MQ=255)

                                     gATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGAtt                                                >  1:1891542/1‑43 (MQ=255)

                                     gATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGAtt                                                >  1:2345562/1‑43 (MQ=255)

                                     gATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGATTTCGATATCCAGCCAGACAGGTGGCTCCt                    <  1:3293439/71‑1 (MQ=255)

                                     gATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGATTTCGATATCCAGCCAGACAGGTGGCTCCt                    <  1:1279782/71‑1 (MQ=255)

                                       ttttGCGCTGAATATCATGCAGATGCTCATCAGTAGTGATTTCGATATCCAGCCAGACAGGTGGCTCCTGc                  <  1:3265129/71‑1 (MQ=255)

                                       ttttGCGCTGAATATCATGCAGATGCTCATCAGTAGTGATTTCGATATCCAGCCAGACAGGTGGCTCCTGc                  <  1:4658442/71‑1 (MQ=255)

                                          tGCGCTGAATATCATGCAGATGCTCATCAGTAGTGATTTCGATATCCAGCCAGACAGGTGGCTCCTGCGa                <  1:4485727/70‑1 (MQ=255)

                                          tGCGCTGAATATCATGCAGATGCTCATCAGTAGTGATTTCGATATCCAGCCAGACAGGTGGCTCCTGCGa                <  1:4610191/70‑1 (MQ=255)

                                          tGCGCTGAATATCATGCAGATGCTCATCAGTAGTGATTTCGATATCCAGCCAGACAGGTGGCTCCTGCGa                <  1:4243694/70‑1 (MQ=255)

                                          tGCGCTGAATATCATGCAGATGCTCATCAGTAGTGATTTCGATATCCAGCCAGACAGGTGGCTCCTGCGa                <  1:1027151/70‑1 (MQ=255)

                                           gcgcTGAATATCATGCAGATGCTCATCAGTAGTGATTTCGATATCCAGCCAGACAGGTGGCTCCTGCGa                <  1:3158795/69‑1 (MQ=255)

                                            cgcTGAATATCATGCAGATGCTCATCAGTAGTGATTTCTATATCCAGCCAGACAGGTGGCTCCTGCGATa              <  1:538182/70‑1 (MQ=255)

                                            cgcTGAATATCATGCAGATGCTCATCAGTAGTGATTTCGATATCCAGCCAGACAGGTGGCTCCTGCGATa              <  1:422714/70‑1 (MQ=255)

                                            cgcTGAATATCATGCAGATGCTCATCAGTAGTGATTTCGATATCCAGCCAGACAGGTGGCTCCTGCGATa              <  1:1163293/70‑1 (MQ=255)

                                            cgcTGAATATCATGCAGATGCTCATCAGTAGTGATTTCGATATCCAGCCAGACAGGTGGCTCCTGCGATa              <  1:2991711/70‑1 (MQ=255)

                                               tGAATATCATGCAGATGCTCATCAGTAGTGATTTCg                                             <  1:4696467/36‑1 (MQ=255)

                                               tGAATATCATGCAGATGCTCATCAGTAGTGATTTCg                                             <  1:4870123/36‑1 (MQ=255)

                                               tGAATATCATGCAGATGCTCATCAGTAGTGATTTCg                                             <  1:1198433/36‑1 (MQ=255)

                                               tGAATATCATGCAGATGCTCATCAGTAGTGATTTCg                                             <  1:702162/36‑1 (MQ=255)

                                               tGAATATCATGCAGATGCTCATCAGTAGTGATTTCg                                             <  1:876735/36‑1 (MQ=255)

                                               tGAATATCATGCAGATGCTCATCAGTAGTGATTTCg                                             <  1:1027501/36‑1 (MQ=255)

                                               tGAATATCATGCAGATGCTCATCAGTAGTGATTTCg                                             <  1:425201/36‑1 (MQ=255)

                                               tGAATATCATGCAGATGCTCATCAGTAGTGATTTCg                                             <  1:3956581/36‑1 (MQ=255)

                                               tGAATATCATGCAGATGCTCATCAGTAGTGATTTCg                                             <  1:1811024/36‑1 (MQ=255)

                                               tGAATATCATGCAGATGCTCATCAGTAGTGATTTCg                                             <  1:3634465/36‑1 (MQ=255)

                                               tGAATATCATGCAGATGCTCATCAGTAGTGATTTCg                                             <  1:1878387/36‑1 (MQ=255)

                                               tGAATATCATGCAGATGCTCATCAGTAGTGATTTCg                                             <  1:318455/36‑1 (MQ=255)

                                               tGAATATCATGCAGATGCTCATCAGTAGTGATTTCg                                             <  1:2893530/36‑1 (MQ=255)

                                               tGAATATCATGCAGATGCTCATCAGTAGTGATTTCg                                             <  1:2385779/36‑1 (MQ=255)

                                                gAATATCATGCAGATGCTCATCAGTAGTGATTTCGATATCCAGCCAGACAGGTGGCTCCTGCGATACATcg         >  1:1336051/1‑71 (MQ=255)

                                                gAATATCATGCAGATGCTCATCAGTAGTGATTTCGATATCCAGCCAGACAGGTGGCTCCTGCGATACATcg         >  1:3958588/1‑71 (MQ=255)

                                                gAATATCATGCAGATGCTCATCAGTAGTGATTTCGATATCCAGCCAGACAGGTGGCTCCTGCGATACATcg         >  1:1682103/1‑71 (MQ=255)

                                                gAATATCATGCAGATGCTCATCAGTAGTGATTTCGATATCCAGCCAGACAGGTGGCTCCTGCGATACATcg         >  1:1777829/1‑71 (MQ=255)

                                                gAATATCATGCAGATGCTCATCAGTAGTGATTTCGATATCCAGCCAGACAGGTGGCTCCTGCGATACATcg         >  1:2027317/1‑71 (MQ=255)

                                                gAATATCATGCAGATGCTCATCAGTAGTGATTTCGATATCCAGCCAGACAGGTGGCTCCTGCGATACATcg         >  1:731633/1‑71 (MQ=255)

                                                       aTGCAGATGCTCATCAGTAGTGATTTCGATATCCAGCCAGACAGGTGGCTCCTGCGATACATCGCGCCACt  >  1:3645459/1‑71 (MQ=255)

                                                       aTGCAGATGCTCATCAGTAGTGATTTCGATATCCAGCCAGACAGGTGGCTCCTGCGATACATCGCGCCACt  >  1:3639669/1‑71 (MQ=255)

GCAATACTTCGACAGGCAATGATTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATACTCATCAGTAGTGATTTCGATATCCAGCCAGACAGGTGGCTCCTGCGATACATCGCGCCACT  >  minE/244413‑244538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`