Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	286,334	C→T	T147I (ACA→ATA)	clpX →	ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	286,334	0	C	T	100.0%	241.2 / NA	74	T147I (ACA→ATA)	clpX	ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/41);  total (33/41)

AAACGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTACACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTGAAAACATCATTCAGAAGCTGTTGCAGAAATGC  >  minE/286268‑286401                                                                   |                                                                    aaaCGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACTCGACTATACTg                                                                 <  1:3898106/71‑1 (MQ=255) aaaCGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTATACTg                                                                 <  1:1988270/71‑1 (MQ=255) aaaCGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTATACTg                                                                 <  1:3285967/71‑1 (MQ=255) aaaCGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTATACTg                                                                 <  1:3166528/71‑1 (MQ=255) aaaCGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTATACTg                                                                 <  1:3746295/71‑1 (MQ=255) aaaCGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTATACTg                                                                 <  1:2516929/71‑1 (MQ=255) aaaCGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTATACTg                                                                 <  1:2353424/71‑1 (MQ=255) aaaCGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTATACTg                                                                 <  1:224528/71‑1 (MQ=255) aaaCGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTATACTg                                                                 <  1:2150564/71‑1 (MQ=255) aaaCGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTATACTg                                                                 <  1:430461/71‑1 (MQ=255) aaaCGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTATACTg                                                                 <  1:4310964/71‑1 (MQ=255) aaaCGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTATACTg                                                                 <  1:1914393/71‑1 (MQ=255) aaaCGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTATACTg                                                                 <  1:4470319/71‑1 (MQ=255) aaaCGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTATACTg                                                                 <  1:4473874/71‑1 (MQ=255) aaaCGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTATACTg                                                                 <  1:499288/71‑1 (MQ=255) aaaCGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTATACTg                                                                 <  1:951121/71‑1 (MQ=255) aaaCGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTATACTg                                                                 <  1:140114/71‑1 (MQ=255) aaaCGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTATACTg                                                                 <  1:821700/71‑1 (MQ=255) aaaCGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTATACTg                                                                 <  1:76458/71‑1 (MQ=255) aaaCGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTATACTg                                                                 <  1:624828/71‑1 (MQ=255)         ctggctgAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTATACTGACCGaa                                                           <  1:94063/69‑1 (MQ=255)         ctggctgAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTATACTGACCGaa                                                           <  1:1662718/69‑1 (MQ=255)         ctggctgAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTATACTGACCGaa                                                           <  1:2929696/69‑1 (MQ=255)         ctggctgAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTATACTGACCGaa                                                           <  1:3658747/69‑1 (MQ=255)         ctggctgAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTATACTGACCGaa                                                           <  1:851180/69‑1 (MQ=255)         ctggctgAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTATACTGACCGaa                                                           <  1:846907/69‑1 (MQ=255)         ctggctgAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTATACTGACCGaa                                                           <  1:2312309/69‑1 (MQ=255)            gctgAAAGGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTATACTGACCGAAGCCg                                                       <  1:2237439/70‑1 (MQ=255)            gctgAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTATACTGACCGAAGCCg                                                       <  1:1136171/70‑1 (MQ=255)             ctgAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTATACTGACCGAAGCCGGt                                                     >  1:3352311/1‑71 (MQ=255)             ctgAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTATACTGACCGAAGCCGGt                                                     >  1:3922571/1‑71 (MQ=255)                  aCGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTATACTGACCGAAGCCGGTTAtgt                                                <  1:50201/71‑1 (MQ=255)                       ggCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTATACTGACCGAAGCCGGTTATGTGGGt                                            >  1:907672/1‑70 (MQ=255)                       ggCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTATACTGACCGAAGCCGGTTATGTGGGt                                            >  1:2702793/1‑70 (MQ=255)                                gctgGATGTTCCGTTCACCATGGCCGACGCGACTATACTGACCGAAGCCGGTTATGTGGGTGAAGACGtt                                   >  1:999302/1‑70 (MQ=255)                                gctgGATGTTCCGTTCACCATGGCCGACGCGACTATACTGACCGAAGCCGGTTATGTGGGTGAAGACGtt                                   >  1:3596899/1‑70 (MQ=255)                                gctgGATGTTCCGTTCACCATGGCCGACGCGACTATACTGACCGAAGCCGGTTATGTGGGTGAAGACGtt                                   >  1:3326227/1‑70 (MQ=255)                                gctgGATGTTCCGTTCACCATGGCCGACGCGACTATACTGACCGAAGCCGGTTATGTGGGTGAAGACGtt                                   >  1:1113230/1‑70 (MQ=255)                                gctgGATGTTCCGTTCACCATGGCCGACGCGACTATACTGACCGAAGCCGGTTATGTGGGTGAAGACGtt                                   >  1:1209690/1‑70 (MQ=255)                                gctgGATGTTCCGTTCACCATGGCCGACGCGACTATACTGACCGAAGCCGGTTATGTGGGTGAAGACGtt                                   >  1:262740/1‑70 (MQ=255)                                gctgGATGTTCCGTTCACCATGGCCGACGCGACTATACTGACCGAAGCCGGTTATGTGGGTGAAGACGtt                                   >  1:2608272/1‑70 (MQ=255)                                gctgGATGTTCCGTTCACCATGGCCGACGCGACTATACTGACCGAAGCCGGTTATGTGGGTGAAGACGtt                                   >  1:2604127/1‑70 (MQ=255)                                gctgGATGTTCCGTTCACCATGGCCGACGCGACTATACTGACCGAAGCCGGTTATGTGGGTGAAGACGtt                                   >  1:148729/1‑70 (MQ=255)                                    gATGTTCCGTTCACCATGGCCGACGCGACTATACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTGaa                                <  1:4720173/69‑1 (MQ=255)                                    gATGTTCCGTTCACCATGGCCGACGCGACTATACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTGaa                                <  1:4788905/69‑1 (MQ=255)                                    gATGTTCCGTTCACCATGGCCGACGCGACTATACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTGaa                                <  1:1678550/69‑1 (MQ=255)                                    gATGTTCCGTTCACCATGGCCGACGCGACTATACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTGaa                                <  1:1508598/69‑1 (MQ=255)                                    gATGTTCCGTTCACCATGGCCGACGCGACTATACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTGaa                                <  1:1941269/69‑1 (MQ=255)                                    gATGTTCCGTTCACCATGGCCGACGCGACTATACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTGaa                                <  1:2022126/69‑1 (MQ=255)                                    gATGTTCCGTTCACCATGGCCGACGCGACTATACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTGaa                                <  1:2059541/69‑1 (MQ=255)                                    gATGTTCCGTTCACCATGGCCGACGCGACTATACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTGaa                                <  1:1458310/69‑1 (MQ=255)                                    gATGTTCCGTTCACCATGGCCGACGCGACTATACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTGaa                                <  1:2921820/69‑1 (MQ=255)                                       gTTCCGTTCACCATGGCCGACGCGACTATACTGACCGaa                                                           <  1:1406309/39‑1 (MQ=255)                                               cACCATGGCCGACGCGACTATACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTGAAAAcatcat                        >  1:3253878/1‑66 (MQ=255)                                               cACCATGGCCGACGCGACTATACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTGAAAACATCATTCa                     >  1:436664/1‑69 (MQ=255)                                               cACCATGGCCGACGCGACTATACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTGAAAACATCATTCAg                    >  1:586186/1‑70 (MQ=255)                                               cACCATGGCCGACGCGACTATACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTGAAAACATCATTCAg                    >  1:1651173/1‑70 (MQ=255)                                               cACCATGGCCGACGCGACTATACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTGAAAACATCATTCAg                    >  1:1023033/1‑70 (MQ=255)                                               cACCATGCCCGACGCGACTATACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTGAAAACATCATTCa                     >  1:38064/1‑69 (MQ=255)                                                      gCCGACGCGACTATACTGACCGAAGCCGGTTATGTGGGTGAAGACTTTGAAAACATCATTCAGAAGCTGtt            >  1:3902970/1‑71 (MQ=255)                                                      gCCGACGCGACTATACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTg                                  >  1:3446241/1‑49 (MQ=255)                                                      gCCGACGCGACTATACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTGAAAATATCATTCAGAAGCTGtt            >  1:1388555/1‑71 (MQ=255)                                                      gCCGACGCGACTATACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTGAAAACATCATTCAGAAGCTGtt            >  1:4705034/1‑71 (MQ=255)                                                      gCCGACGCGACTATACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTGAAAACATCATTCAGAAGCTGtt            >  1:4510993/1‑71 (MQ=255)                                                      gCCGACGCGACTATACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTGAAAACATCATTCAGAAGCTGtt            >  1:4468745/1‑71 (MQ=255)                                                      gCCGACGCGACTATACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTGAAAACATCATTCAGAAGCTGtt            >  1:561387/1‑71 (MQ=255)                                                      gCCGACGCGACTATACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTGAAAACATCATTCAGAAGCTGtt            >  1:4073609/1‑71 (MQ=255)                                                      gCCGACGCGACTATACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTGAAAACATCATTCAGAAGCTGtt            >  1:647595/1‑71 (MQ=255)                                                      gCCGACGCGACTATACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTGAAAACATCATTCAGAAGCTGtt            >  1:2833494/1‑71 (MQ=255)                                                      gCCGACGCGACTATACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTGAAAACATCATTCAGAAGCTGtt            >  1:1302849/1‑71 (MQ=255)                                                      gCCGACGCGACTATACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTGAAAACATCATTCAGAAGCTGtt            >  1:129394/1‑71 (MQ=255)                                                      gCCGACGCGACTATACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTGAAAACATCATTCAGAAGCTGtt            >  1:3190536/1‑71 (MQ=255)                                                      gCCGACGCGACTATACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTGAAAACATCATTCAGAAGCTGt             >  1:3307986/1‑70 (MQ=255)                                                                cTATACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTGAAAACATCATTCAGAAGCTGTTGCAGAAATGc  <  1:1854026/71‑1 (MQ=255)                                                                   |                                                                    AAACGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTACACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTGAAAACATCATTCAGAAGCTGTTGCAGAAATGC  >  minE/286268‑286401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑