| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,036,505 | G→A | 100% | A632A (GCG→GCA) | rsxC → | fused predicted 4Fe‑4S ferredoxin‑type protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,036,505 | 0 | G | A | 96.0% | 63.2 / ‑3.3 | 25 | A632A (GCG→GCA) | rsxC | fused predicted 4Fe‑4S ferredoxin‑type protein |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (18/6); total (18/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCG > minE/1036437‑1036572 | gCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcacg > 1:2655948/1‑71 (MQ=21) aaCCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAg < 1:2318068/71‑1 (MQ=34) aCCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGc > 1:2663386/1‑71 (MQ=21) ccAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcacg < 1:978317/65‑1 (MQ=25) cAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTg > 1:2441878/1‑71 (MQ=21) agaagtACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTgg < 1:2637761/71‑1 (MQ=255) aagaaCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGaa > 1:1773035/1‑71 (MQ=21) gaaCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGaa > 1:714160/1‑69 (MQ=14) cgTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaac < 1:316779/71‑1 (MQ=255) gAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaacaa > 1:2617595/1‑69 (MQ=255) gAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaacaa > 1:369504/1‑69 (MQ=255) gAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaacaa > 1:2212434/1‑69 (MQ=255) cGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCg < 1:1362665/71‑1 (MQ=255) gCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCcgcg > 1:524159/1‑71 (MQ=255) gCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCcgcg > 1:873716/1‑71 (MQ=255) gTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaa > 1:1417007/1‑50 (MQ=255) tGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAg > 1:2595571/1‑71 (MQ=255) gCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAgc > 1:2460743/1‑71 (MQ=255) ccAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaac > 1:48219/1‑48 (MQ=255) cAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGa < 1:2428524/45‑1 (MQ=255) aaaGCACGCAAGCTGGAACAGGAACAGGCTAATGCCGAGCCAGAACAACAGGTCGa < 1:1274984/56‑1 (MQ=14) aaaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCcgc > 1:770345/1‑61 (MQ=255) aaaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAgccg > 1:2032220/1‑70 (MQ=255) aGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaac < 1:2198109/44‑1 (MQ=255) aGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGCACAACAGGTCGATCCGCGCAAAGccgccg > 1:2274208/1‑71 (MQ=255) aGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGccgccg > 1:2933068/1‑71 (MQ=255) aGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGccgccg > 1:2959899/1‑71 (MQ=255) | GCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCG > minE/1036437‑1036572 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |