Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 915,215 T→G 8.6% intergenic (‑358/+137) ybjE ← / ← aqpZ putative transporter/aquaporin Z

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913915,2150TG 8.6% 300.9 / 2.8 119intergenic (‑358/+137)ybjE/aqpZputative transporter/aquaporin Z
Reads supporting (aligned to +/- strand):  ref base T (79/27);  new base G (2/11);  total (81/38)
Fisher's exact test for biased strand distribution p-value = 5.28e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 127 total aligned reads displayed.

GGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTT  >  NC_000913/915066‑915363
                                                                                                                                                      |                                                                                                                                                     
ggggCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATACGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGAtt                                                                                                                                                       <  2:1887485/150‑1 (MQ=17)
ggggCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGAtt                                                                                                                                                       <  2:112981/150‑1 (MQ=32)
 gggCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTg                                                                                                                                                      <  1:110212/150‑1 (MQ=32)
 gggCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTg                                                                                                                                                      <  2:3234682/150‑1 (MQ=32)
 gggCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTg                                                                                                                                                      <  2:1910374/150‑1 (MQ=32)
 gggCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTg                                                                                                                                                      <  2:1845883/150‑1 (MQ=32)
   gCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGgc                                                                                                                                                    <  1:217068/150‑1 (MQ=32)
   gCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTCACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGgc                                                                                                                                                    <  2:3577259/150‑1 (MQ=17)
     aGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGgccg                                                                                                                                                  >  1:2139012/1‑150 (MQ=32)
      gCCCACCTGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGgccgc                                                                                                                                                 <  2:185200/150‑1 (MQ=17)
      gCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGgccgc                                                                                                                                                 >  1:372529/1‑150 (MQ=32)
      gCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGgccgc                                                                                                                                                 <  1:3249302/150‑1 (MQ=32)
      gCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGgccgc                                                                                                                                                 >  1:2892370/1‑150 (MQ=32)
      gCCCACATCCATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGgccgc                                                                                                                                                 >  2:3322037/1‑150 (MQ=17)
      gCCCACAGGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGGTACCGCGCGCCCGACCCGGTAGCCGTAAGGGATACGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGgccgc                                                                                                                                                 <  1:1247452/150‑1 (MQ=255)
       cccACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGgccgcc                                                                                                                                                >  1:2887995/1‑150 (MQ=32)
       cccACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGgccgcc                                                                                                                                                >  1:2888309/1‑150 (MQ=32)
       cccACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGgccgcc                                                                                                                                                >  1:1598147/1‑150 (MQ=32)
         cacaGGGTTGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGcccc                                                                                                                                              <  1:2092602/150‑1 (MQ=17)
              ggATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTgcg                                                                                                                                        >  2:53672/1‑150 (MQ=32)
              ggATGTGGGCTGAGGGCGCGTTTTAAAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGAATGGCCGCCCCCTgcg                                                                                                                                        >  2:53595/1‑150 (MQ=17)
                      gCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTccat                                                                                                                                >  2:3012395/1‑148 (MQ=17)
                      gCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCTccgt                                                                                                                                >  1:3756487/1‑148 (MQ=17)
                      gCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCTCCtt                                                                                                                                >  2:1455653/1‑150 (MQ=17)
                                 tttttACTGGGTTGTTACCTCGCGCCCGACCGGGTAGCGTTATGGTATATGTCGTGGCCACCGCGCTGCGGCTTTTTTTTACGCAAGAGCCCGGGGGTGCGGGGGGCGGCGGCTTTGGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGc                                                                                                                     <  2:579695/149‑1 (MQ=2)
                                 gTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGc                                                                                                                     >  2:2579567/1‑150 (MQ=33)
                                  ttttACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCa                                                                                                                    >  1:2446567/1‑150 (MQ=34)
                                  ttttACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCTCCATGCGCCAGTGGCa                                                                                                                    >  1:2739283/1‑150 (MQ=21)
                                   tttACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCaa                                                                                                                   >  2:775605/1‑150 (MQ=35)
                                        aGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGt                                                                                                              >  1:253428/1‑150 (MQ=39)
                                        aGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCTACATGCGCCAGTGGCAATATGt                                                                                                              >  1:1897433/1‑150 (MQ=255)
                                          ggATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGGGGCGTTTTGCCGCCCCCTGGGCGCTCCTTGCGCCAGTGGCAATATGTTg                                                                                                            >  2:1373214/1‑150 (MQ=255)
                                               ttACCTCGCGCCCGACCGGTTCGCGTTATGGTCAAAGTCGGGGGCACGGCGCCGCGGCTTTTTTTTTCGCCAGGGCCGGGGGGTGCCGGGGGGGTGGGCGTTTGGCCGCCCCCTCCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAg                                                                                                       <  2:1647926/150‑1 (MQ=9)
                                               ttACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTTTTCGCCAGAGCCCGGGGGTGCCGGGGGCGGCGGGGATTGGCCGCCCCCAGCGCGCTCCTTGCGCCAGTGGCAATAAGTTGCATAg                                                                                                       >  1:3402687/1‑150 (MQ=14)
                                               ttACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTAGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTTGCCGCCCCCAGCCCGCACCCTGCGCCCGTGGCAATAATTTGCTTAg                                                                                                       >  1:3402647/1‑150 (MQ=14)
                                                tACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGCTTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGc                                                                                                      >  2:3735356/1‑150 (MQ=255)
                                                  ccTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTc                                                                                                    >  2:728090/1‑150 (MQ=255)
                                                   cTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCa                                                                                                   >  2:3228785/1‑150 (MQ=255)
                                                   cTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCa                                                                                                   >  2:371296/1‑150 (MQ=255)
                                                   cTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCGTGCGCCAGTGGCAATATGTTGCTTAGCTCa                                                                                                   >  2:371253/1‑150 (MQ=255)
                                                   cTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCa                                                                                                   >  2:371290/1‑150 (MQ=255)
                                                    tCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGCTTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCag                                                                                                  >  1:3879567/1‑149 (MQ=255)
                                                    tCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCCGTGGCAATATGTTGCTTAGCTCAt                                                                                                  >  1:3879605/1‑150 (MQ=255)
                                                    tCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTATCTCAt                                                                                                  >  1:3540203/1‑150 (MQ=255)
                                                    tCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCTCCGTGCGCCCGGGGCAATATGTTGCTTAGCTCAt                                                                                                  >  1:1812638/1‑150 (MQ=255)
                                                    tCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCGCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCAt                                                                                                  >  1:1812619/1‑150 (MQ=255)
                                                    ccgcgCCCCACCCGGTTGCGGTACGGTTTTCGTGGGGGCCACCGCGCTGGGGGTTTTTTTTTCGCCAGCGCCGGGGGGGGCTGGGGGCGGGGGGTTTGGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCAt                                                                                                  <  2:3805790/149‑1 (MQ=12)
                                                     cgcgCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTTCTTAGCTCATg                                                                                                 >  2:1101644/1‑150 (MQ=255)
                                                     cgcgCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATg                                                                                                 >  2:776079/1‑150 (MQ=255)
                                                         cccGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGAGTGGCCGCCCCCAGCGCGCTCCTTGCGCCCGTGGCAATATGTTGCTTTGCGCCTGAAAg                                                                                             >  1:3679929/1‑150 (MQ=14)
                                                           cGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGa                                                                                           >  1:2284225/1‑150 (MQ=255)
                                                            gACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAg                                                                                          >  2:3817314/1‑150 (MQ=255)
                                                            gACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAg                                                                                          >  2:3050960/1‑150 (MQ=255)
                                                                 ggTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATGGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCACCGGAAAGGAGCGcaa                                                                                     >  2:3697319/1‑150 (MQ=255)
                                                                  gTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGcaac                                                                                    >  2:3581451/1‑150 (MQ=255)
                                                                      ccttatgggATACGTCGAGGGCATCGCGCAGCGGCGATTTTGTTCGCCAGAGCCGGGGGGTGCTGGGGGGGGGGGCGTTTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAga                                                                                <  2:1334016/144‑1 (MQ=18)
                                                                        gTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCCGCCATTGGCCCCCCCCTGCGCGCGCCTTTCGCCACTGGCAATAATTTTCTTATCTCATTCAAGGAGCGCAACAAgaat                                                                              >  1:146865/1‑148 (MQ=17)
                                                                         tAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCATGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAgatga                                                                             >  2:357468/1‑150 (MQ=255)
                                                                         tAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCGCCTTGCGCCACTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAgatga                                                                             >  1:3816009/1‑150 (MQ=255)
                                                                           aGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGGCGCCCCCAGCGCGCGCCATGCGCCAGTGGCAAAATTTTTCTTAGCTCAAGAAAGGAGCGCAACAAgatgatt                                                                           >  1:3776378/1‑149 (MQ=17)
                                                                             ggATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGaa                                                                         >  2:3529613/1‑150 (MQ=255)
                                                                             ggATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCTGCGCGCTCCTTGCCCCAGTGGCAAAAAGTTTCTTATCTCATGAAAGGAGCGCAAAAAGAATATGAAt                                                                        >  2:2835342/1‑150 (MQ=17)
                                                                                 acgccgtgggCACCGCGCCGCGGCTTTTTTTTTCGCCAGAGCCGGGGGGTGCTGGGGGCGGCGGCGTTTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCaa                                                                     <  2:1997124/143‑1 (MQ=18)
                                                                                 aaGTCGTGGGCACCGCGCAGCGGCGTTTTTGTTCGCCAGAGCCGGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCaa                                                                     <  2:2281277/150‑1 (MQ=255)
                                                                                    tCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGCTTGGCCGCCCCCAGCGCCCGCCCTGCGCCCCTGGCAAAATGTTTCATATCTCAGGGAAGGAGCGCAACAAGAAGAATAATAAACaa                                                                  >  1:3529964/1‑149 (MQ=14)
                                                                                    tCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACat                                                                  >  2:2613928/1‑150 (MQ=255)
                                                                                    tCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCCCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGAAGATGAATCAACat                                                                  >  2:3015331/1‑150 (MQ=255)
                                                                                            aCCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCATGAGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCCACAAGATGATGAATCAACAAATaacaac                                                          >  2:813836/1‑150 (MQ=255)
                                                                                              cgcgCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACAt                                                        >  2:1735789/1‑150 (MQ=255)
                                                                                               gcgcAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATc                                                       >  2:807614/1‑150 (MQ=255)
                                                                                                   cgcggcgTTTTTGTTCGCCAGCGCCGGGGGGTGCTGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTaa                                                   <  2:3956706/149‑1 (MQ=255)
                                                                                                    ggggcttttttGTTCGCCTGAGCGGGGGGGGGGGGGGGGGGGCGGCTTTGGCCCGCCCCCCGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTaaa                                                  <  1:3289126/143‑1 (MQ=255)
                                                                                                       gcgATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGACTCAACATATAACAACATCTTaaaaaa                                               >  2:928315/1‑150 (MQ=255)
                                                                                                        tttttttGTTCGCCAGTGCGGGGGGGTGCGGGGGGGGGGGGCTTTGGCCCGCCCCCGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTaaaaaaa                                              <  2:2759414/147‑1 (MQ=255)
                                                                                                        cttttttGTTCGCCAGCGCCGGGGGGTGCTGGGGGGGGGGGCGATTGGCGGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTaaaaaaa                                              <  2:2130842/147‑1 (MQ=25)
                                                                                                         ttttttGTCCGCCGGCGCGGGGGGGGGGGGGGGGCGGGGGGGTGGGCCCCCCCCCCCCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAg                                             <  1:25731/148‑1 (MQ=255)
                                                                                                         gtttttGTTCGCCAGAGCCCGGGGGTGCTGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAg                                             <  2:2972112/148‑1 (MQ=255)
                                                                                                         gATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAg                                             >  2:122581/1‑150 (MQ=255)
                                                                                                          tttttGTTCGCCTGTGCCGGGGGGTGCTGGGGGGGGGGGGTTTTGGCCGCCCCCGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAgg                                            <  2:3491647/149‑1 (MQ=25)
                                                                                                           ttttttTCGCCTGCGCCGGGGGGTGCGGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGc                                           <  2:3920946/150‑1 (MQ=25)
                                                                                                           ttttGTTCGCCAGAGCCCGGGGGTGCAGGGGGTGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGc                                           <  2:883642/150‑1 (MQ=255)
                                                                                                             ttGTTCGCCAGAGCCCGGGGGGGCCGGGGGGGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCt                                         <  2:3946399/150‑1 (MQ=255)
                                                                                                                  cGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACAt                                    >  2:651283/1‑150 (MQ=255)
                                                                                                                    ccAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCATGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAAAATCTTAAAAAAAGGCCTGAAATTa                                  >  2:1469379/1‑150 (MQ=255)
                                                                                                                     cAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATGGGCCGCCCCCTGCGCGCTCCGTGCGCCAGGGGCAATATGTTGCGTAGCTCATGACAGGAGCGCAACAAGATGAAGCAGCAACATATCACAACATCTTCCAAAAAGGCCTGAccgtcc                                 >  2:3519878/1‑145 (MQ=18)
                                                                                                                     cAGAGCCCGGGGGTGCAGGGGGCGGCGGCGAGTGGCCGCCCCCTGCGCGCGCCTTGCGCCCGGGGCAAGATGGTGCGTCGCGCCGGAAAGGAGCGCACCAAGATGAGGACTCAACCTAGAACACCCGCTTACAAAAAGGCCTGACagtcc                                 >  2:3519876/1‑146 (MQ=12)
                                                                                                                        ttgcggggggTGTGGGGGGGGGGGGGGTTGTGGCCCCCCCGGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGc                               <  2:3402647/145‑1 (MQ=255)
                                                                                                                           ccGGGTGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAgg                           >  2:2610340/1‑150 (MQ=255)
                                                                                                                                   gggggggggggggGCGGTGGGCCCCCCCCGGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATGTAACAACATCTTAAAAAAAGGCCTGGCGTAATGTCAGGCCttttt                    <  2:1159784/147‑4 (MQ=255)
                                                                                                                                      ggggggggCGGCGTTGGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGtt                <  2:2887995/150‑1 (MQ=255)
                                                                                                                                      gggggCGGCGGCTATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGtt                <  2:1598147/150‑1 (MQ=255)
                                                                                                                                       ggggCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGtta               >  1:3333389/1‑150 (MQ=255)
                                                                                                                                        gggCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGttaa              >  1:3331629/1‑150 (MQ=255)
                                                                                                                                          ggggcggccAATCGCCGCCGCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGttaatt            >  2:251787/3‑150 (MQ=255)
                                                                                                                                          gcgggggcgATTGGCCGCCCCCGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGttaatt            <  2:1288777/150‑1 (MQ=255)
                                                                                                                                                 gttttGGCCGCCCCGTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACg     <  2:3816009/147‑1 (MQ=255)
                                                                                                                                                    ttGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCtt  >  2:2567022/1‑150 (MQ=255)
                                                                                                                                                    ttGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCtt  >  2:1611465/1‑150 (MQ=255)
                                                                                                                                                    ttGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCtt  >  1:1463559/1‑150 (MQ=255)
                                                                                                                                                    ttGGCCGCCCACTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAATGGCCTGACATTACTCCAGGCCTTCTGCGTTAATTAATCACGCtt  >  2:1920430/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
GGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTT  >  NC_000913/915066‑915363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: