Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,090,124 C→G 5.1% intergenic (+78/‑68) hisL → / → hisG his operon leader peptide/ATP phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,090,1240CG 5.1% 619.7 / 17.0 172intergenic (+78/‑68)hisL/hisGhis operon leader peptide/ATP phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (93/70);  new base G (6/3);  total (99/73)
Fisher's exact test for biased strand distribution p-value = 7.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 174 total aligned reads displayed.

TAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAATTGCTGGCGCGC  >  NC_000913/2089975‑2090272
                                                                                                                                                     |                                                                                                                                                    
tAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATc                                                                                                                                                      <  1:298991/150‑1 (MQ=255)
 aaaCATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCa                                                                                                                                                     <  2:649388/150‑1 (MQ=255)
     aTTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCACCCGGAAGATCACCtt                                                                                                                                                 >  1:351546/1‑150 (MQ=255)
      ttCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTc                                                                                                                                                >  2:1084804/1‑150 (MQ=255)
           agagACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCgggg                                                                                                                                           <  1:3170109/150‑1 (MQ=255)
           agagACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTCCCgggg                                                                                                                                           <  1:651688/150‑1 (MQ=255)
           agagACTTTTATGAAACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGGTGATCTTCCgggg                                                                                                                                           <  1:418859/150‑1 (MQ=255)
                    tATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTa                                                                                                                                  <  2:2790998/150‑1 (MQ=255)
                    tATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTACTCTTTCCGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCGTCCGGGGGCTTTTTTa                                                                                                                                  <  1:2022595/150‑1 (MQ=255)
                      tGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTAtt                                                                                                                                <  2:1976069/150‑1 (MQ=255)
                      tGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTAtt                                                                                                                                <  2:861231/150‑1 (MQ=255)
                       gACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTg                                                                                                                               >  2:3489301/1‑150 (MQ=255)
                        acacGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgc                                                                                                                              >  1:1041085/1‑150 (MQ=255)
                          acGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGACGATCACCTTCCGGGGGCTTTTTTATTgcgc                                                                                                                            >  1:2285088/1‑150 (MQ=255)
                          acGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGAGGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTGATTgcgc                                                                                                                            >  2:3931009/1‑150 (MQ=255)
                           cgcgTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGcgcg                                                                                                                           <  1:2968642/150‑1 (MQ=255)
                           cgcgTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGcgcg                                                                                                                           <  2:2845428/150‑1 (MQ=255)
                               ttCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTg                                                                                                                       <  1:3705533/150‑1 (MQ=255)
                                  aaTTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATa                                                                                                                    >  1:2683025/1‑150 (MQ=255)
                                   aTTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATaa                                                                                                                   >  1:179959/1‑150 (MQ=255)
                                    tttAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAAc                                                                                                                  >  1:3681792/1‑150 (MQ=255)
                                    tttAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAAc                                                                                                                  >  1:3868681/1‑150 (MQ=255)
                                     ttAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTCGTGCGCGGTTGATAACg                                                                                                                 >  2:1881472/1‑150 (MQ=255)
                                      tAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACgg                                                                                                                <  1:2084456/150‑1 (MQ=255)
                                      tAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACgg                                                                                                                <  2:1736252/150‑1 (MQ=255)
                                          caccacCATCATCACCATCCTCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCa                                                                                                            <  2:3433153/150‑1 (MQ=255)
                                           accaccATCATCACCATCATCCGGACTAGTCTTTCAGGCGAGGGGTGCTGGAAGACATTCAGATCTTCCCGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTGTTGAGTGCGCGGTTGATCACGGTTctg                                                                                                           >  2:3373135/1‑148 (MQ=255)
                                             caccatcatcaccatcatCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATGACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCCGAc                                                                                                         >  2:1424647/1‑150 (MQ=255)
                                             caccatcatcaccatcatCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGAc                                                                                                         >  1:922650/1‑150 (MQ=255)
                                               ccatcatcaccatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAg                                                                                                       <  1:237728/150‑1 (MQ=255)
                                               ccatcatcaccatcatcCTGACTAGTCTGTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAg                                                                                                       <  2:3720464/150‑1 (MQ=255)
                                                 atcatcaccatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGt                                                                                                     <  2:242682/150‑1 (MQ=255)
                                                 atcatcaccatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGt                                                                                                     <  1:2528898/150‑1 (MQ=255)
                                                  tcatcaccatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGtt                                                                                                    >  1:3534793/1‑150 (MQ=255)
                                                    atcaccatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGt                                                                                                     >  1:1917896/1‑147 (MQ=255)
                                                    atcaccatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTa                                                                                                  >  1:2578353/1‑150 (MQ=255)
                                                     tcaccatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTaa                                                                                                 >  1:3027185/1‑150 (MQ=255)
                                                     tcaccatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTaa                                                                                                 <  1:1001104/150‑1 (MQ=255)
                                                           tcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGa                                                                                           >  1:3888643/1‑150 (MQ=255)
                                                             atcCTGACTAGTCTTTCAGGCGAGGTGTGCTGGAAGACCTTCAGATCTTCCAGGGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTGTAGTGCGCGGTTGATACCGGTTCAGACAGGGTGAAAGAGGact                                                                                         >  2:2788856/1‑148 (MQ=255)
                                                              tcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATa                                                                                        >  2:828581/1‑150 (MQ=255)
                                                              tcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATa                                                                                        >  1:3678606/1‑150 (MQ=255)
                                                                   aCTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCAAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCTGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACaaa                                                                                   >  1:2385824/1‑150 (MQ=255)
                                                                    cTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACaaaa                                                                                  <  2:3313254/150‑1 (MQ=255)
                                                                        tCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATgac                                                                              >  1:1241294/1‑150 (MQ=255)
                                                                        tCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATgac                                                                              <  2:179959/150‑1 (MQ=255)
                                                                        tCTTTCAGGCGAGGTGTGCTGGAAGACATTCAGATCTTCCAGGGGTGCATGAACGCATGAGAAAGCCCCCGGACGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATgac                                                                              >  2:3030876/1‑150 (MQ=255)
                                                                         cTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATgaca                                                                             <  1:806382/150‑1 (MQ=255)
                                                                          tttCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATCACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATgacag                                                                            >  1:2797809/1‑150 (MQ=255)
                                                                          tttCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATgacag                                                                            >  1:2973054/1‑150 (MQ=255)
                                                                          tttCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATgacag                                                                            >  2:2750938/1‑150 (MQ=255)
                                                                          tttCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGCTTCAGACAGGTTTAAAGAGGAATAACAAAATgacag                                                                            >  1:3155355/1‑150 (MQ=255)
                                                                             gaGGCGATGTGTGCTGGAAGACATTCAGATCTTCGAGTTGTGCATGAACGCATGATAAAGCCACCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACATTATgacagtca                                                                         <  2:3844228/149‑1 (MQ=255)
                                                                                gCGATGTGTGCTGGAAGACATTCAGCTCTTCCAGTTGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCAACTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGacaaca                                                                      <  1:2508699/150‑1 (MQ=255)
                                                                                        tgCTGGAAGACAGTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTa                                                              <  1:3759957/150‑1 (MQ=255)
                                                                                           tGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTCATTGCGAGGTTGATAACGGTTCAGACAGGATTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTAAGc                                                           >  2:2470770/1‑150 (MQ=255)
                                                                                                gACATTCAGATCTTCCAGTGGTGCATGAACGCAGGATAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAAAGACAGACAACACTCGTTTACGCATAGc                                                      >  2:2261583/1‑150 (MQ=255)
                                                                                                   aTTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTAAGCATATCTAt                                                   >  1:2242969/1‑150 (MQ=255)
                                                                                                    ttCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATg                                                  >  1:3411064/1‑150 (MQ=255)
                                                                                                    ttCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTATACGCATAGCTATg                                                  >  1:529864/1‑150 (MQ=255)
                                                                                                     tCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTATAGAGGAATAACAAAAAGACAGACAACACTCGTTTACGCATAGCTATGc                                                 >  1:1675096/1‑150 (MQ=255)
                                                                                                     tCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGc                                                 >  2:1358001/1‑150 (MQ=255)
                                                                                                     tCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGc                                                 >  2:1949795/1‑150 (MQ=255)
                                                                                                       aGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAg                                               >  1:1828809/1‑150 (MQ=255)
                                                                                                        gATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGa                                              >  2:384715/1‑150 (MQ=255)
                                                                                                         aTCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGaa                                             >  2:3456896/1‑150 (MQ=255)
                                                                                                         aTCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCATTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGaa                                             <  1:1620745/150‑1 (MQ=255)
                                                                                                            ttCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATc                                          >  2:1337137/1‑150 (MQ=255)
                                                                                                             tCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATcc                                         >  2:2937637/1‑150 (MQ=255)
                                                                                                             tCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATcc                                         >  2:886265/1‑150 (MQ=255)
                                                                                                             tCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATcc                                         >  2:1193997/1‑150 (MQ=255)
                                                                                                               gaGTGGTGCATGAACGAATGAGAAAGCCCCCTGAAGATCACCTTCCGGGTGCTTTTTTATTGCGGGGTTGATAACGTTTCAGACAGGTTTAAAGTGGAATAACATAATGTCAGACAACACTCGTTTACGCATATCTTTGCAGTATTCCgg                                       <  2:599499/149‑1 (MQ=255)
                                                                                                               cAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCgg                                       <  2:599473/150‑1 (MQ=255)
                                                                                                               cAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCgg                                       <  2:3314562/150‑1 (MQ=255)
                                                                                                                   ggtgCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGt                                   <  2:2964840/150‑1 (MQ=255)
                                                                                                                     tgCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGttt                                 >  2:2150037/1‑150 (MQ=255)
                                                                                                                        aTGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAg                              >  2:2015988/1‑150 (MQ=255)
                                                                                                                        aTGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAg                              >  2:3280879/1‑150 (MQ=255)
                                                                                                                        aTGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTGCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGACATCCGGCCGTTTAAg                              >  2:1232574/1‑150 (MQ=255)
                                                                                                                            aCGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGtgat                          <  2:723589/150‑1 (MQ=255)
                                                                                                                               cATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGAc                       >  2:3319360/1‑150 (MQ=255)
                                                                                                                                aTGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACt                      <  1:1707538/150‑1 (MQ=255)
                                                                                                                                 tGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTCATGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTGAAGTGATGACTc                     >  2:444594/1‑150 (MQ=255)
                                                                                                                                       aGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTAAGACAGGTTTAAAGTGGAATAAAAAAATGTCAGACCACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGGTGTCTCAcccgc               >  1:504702/1‑149 (MQ=255)
                                                                                                                                        gCCCCCGGAAGGTGATCTTCCGGGGGCTTTCTTATTGCGCGGGTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAAAGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGGTGTCTCACGCGaa              >  1:3053240/1‑150 (MQ=255)
                                                                                                                                        gCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAAGGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGaa              >  2:2386432/1‑150 (MQ=255)
                                                                                                                                        ccccccGGACGAGCACCGGCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTACAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACACACGCGaa              <  2:2497309/149‑1 (MQ=255)
                                                                                                                                         cccccGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAAt             <  1:828581/150‑1 (MQ=255)
                                                                                                                                          ccccGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCCGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTTAGTGATGACTCACGCGAAtt            >  1:1241272/1‑150 (MQ=255)
                                                                                                                                           cccGGAAGGTGATCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGCATAACAAAATGACAGACAACACACGTTTACGCATAGCTATGCCGAACACCCGCCGGTTAAGGGATGACGCACGCGACTTg           >  1:2824629/1‑150 (MQ=255)
                                                                                                                                           cccGGAAGGTGATCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAAAGGTTGAGACAGGTTTAAAGTGGAATTACAAAATGACAGACAACACTCGTTTACGCATAGCTATTCAGAAATACGGCCGTTTAATTTTTGTCTAAAGCGAAttt           >  1:2722585/1‑149 (MQ=255)
                                                                                                                                           cccGGAAGGTGATCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAAAGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACACGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATTACACAAGCGAAttt           >  1:3444485/1‑149 (MQ=255)
                                                                                                                                            ccGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACCGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCCTAGCTATGCAGAAATCCGGCCGTTTAAGTGATGGCGAACGCGCAtttc          >  1:3836642/1‑148 (MQ=255)
                                                                                                                                            ccGGAAGATCACCTTCCGGGGGCTTTTGTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCTTTTACGCATAGCTATGCACAAATCCGGCCGTATAAGGGGTGACTCACGCGAAtaga          >  2:1916550/1‑147 (MQ=255)
                                                                                                                                             cGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTGTAATTGATGACTCACGCGAATTGCt         >  2:1530445/1‑150 (MQ=255)
                                                                                                                                               gacgATCACCTTCCGGGGGCTTTTTTATTGCGCGTTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAATTGCTgg       <  1:2455555/147‑1 (MQ=255)
                                                                                                                                               gAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAATTGCTgg       <  1:3144917/150‑1 (MQ=255)
                                                                                                                                                  gATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACAAGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAATTGATTACTCACGCGCATTTCTGgcgc    >  1:109954/1‑150 (MQ=255)
                                                                                                                                                   aTCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAATTGCTGgcgcg   >  2:654913/1‑150 (MQ=255)
                                                                                                                                                    tCACCTTCCGGGGGCAATTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAATTGCTGgcgcgc  <  1:2842619/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAATTGCTGGCGCGC  >  NC_000913/2089975‑2090272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: