Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,781,067 T→G 11.2% intergenic (+50/‑148) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,781,0670TG11.2% 399.7 / 2.7 134intergenic (+50/‑148)lldD/trmLL‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base T (34/81);  new base G (16/3);  total (50/84)
Fisher's exact test for biased strand distribution p-value = 9.02e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 134 total aligned reads displayed.

ATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGT  >  NC_000913/3780921‑3781214
                                                                                                                                                  |                                                                                                                                                   
aTCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCg                                                                                                                                                  <  1:2070689/150‑1 (MQ=255)
 tCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCgg                                                                                                                                                 <  2:1392793/150‑1 (MQ=255)
 ccgcgccgCGAAATTCCGCACGCGGCGCGGGGGCCGGGGCTGGGGAAACAGCTGCCTGCGGCACGGGCGCCCATGGCGAAAGGGAACGCGGCCTAGTCGATTGCGCCCCCTCACCCTACCCCACTCCCTCAGGGAGAGGGGACCGTTCgg                                                                                                                                                 <  2:750723/143‑1 (MQ=255)
  cGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGgc                                                                                                                                                <  2:381651/150‑1 (MQ=255)
   gATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGgcg                                                                                                                                               <  1:1848796/150‑1 (MQ=255)
   gATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCAAACCCACACCCCCCCGGGGAGGGGGCCCTgcggcg                                                                                                                                               >  2:3381689/1‑150 (MQ=255)
   gATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCCCACCCAAACCCACTCCCGCCGGGCGGGGGGGCCGGTCGgcg                                                                                                                                               >  1:270959/1‑150 (MQ=255)
   gATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCAAACCCACACCCACACGGGGAGGGGACCGTTCgggg                                                                                                                                               >  1:2267994/1‑148 (MQ=255)
   gATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCAAAACCACTCCCGCAGGGGGAGGGGGCCCTTCGgcg                                                                                                                                               >  1:313267/1‑150 (MQ=255)
   gATCAGCGAAATGACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGgcg                                                                                                                                               <  1:3798212/150‑1 (MQ=255)
    ctCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGGAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGgcgc                                                                                                                                              <  1:1185152/149‑1 (MQ=255)
    aTCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTTCGCCCCCCCACCCAAACCCACACCCACCGGGCGCGGGGACCCGTGGgcgc                                                                                                                                              >  2:818628/1‑150 (MQ=255)
    aTCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGgcgc                                                                                                                                              <  1:1185159/150‑1 (MQ=255)
     tCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCt                                                                                                                                             <  1:2072164/150‑1 (MQ=255)
     tCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCt                                                                                                                                             <  2:3570811/150‑1 (MQ=255)
        gCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCtgta                                                                                                                                          <  1:3352305/150‑1 (MQ=255)
        gCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCACACCCAAAACCAAACCCCCAGGGGGAGGGGGCCCGTAGGCGCcgtt                                                                                                                                          >  2:3061505/1‑149 (MQ=255)
               tACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTc                                                                                                                                   <  2:1489025/150‑1 (MQ=255)
               tACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCCCACCCCAAACCACAACCCCAGGGGGCGGGGGCCCGTAGGCGCgggatgttcgc                                                                                                                                   >  1:79380/1‑146 (MQ=255)
                 cGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTccc                                                                                                                                 <  2:3193040/150‑1 (MQ=255)
                 cGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCACACCCAAACCCACACCCACAGGGGGAGGGGACCGTTCGGCGCGGTATGTACTccc                                                                                                                                 >  1:946283/1‑150 (MQ=255)
                  gCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCACCACCGAAACCACAACCACCGGGCGAGGGGGCCGGTGGGCGGGGTATTTACAcccg                                                                                                                                >  2:1573718/1‑149 (MQ=255)
                    aaGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTTCGCCCCCACACCCAAAACCCCGCCCACACGGAGAGGGGGCCGGTCGGCGCGGTATGTTCTcccgcc                                                                                                                              >  2:1660135/1‑147 (MQ=255)
                            cTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGaaa                                                                                                                      <  1:3950155/150‑1 (MQ=255)
                               gTGCTGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCCCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGa                                                                                                                   <  1:1658391/150‑1 (MQ=255)
                               gTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCAAACCCACTCCCACAGGGAGGGGGGGCCGGTCGGCGCAGTATGTACTCCCACACACAGAAAccc                                                                                                                   >  1:2932220/1‑148 (MQ=255)
                                     gggCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGc                                                                                                             <  2:3461411/150‑1 (MQ=255)
                                        cTGGTTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCACTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCAct                                                                                                          <  1:585982/150‑1 (MQ=255)
                                        cTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCAct                                                                                                          <  1:2458588/150‑1 (MQ=255)
                                         tGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCActc                                                                                                         <  2:3955670/150‑1 (MQ=255)
                                         tGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCACACCCGAACCCACACCCCCCGGGTGGGGGGCCCGGGCGGCGCGGGTGGTACGCCCGCACACTGCACCGGCACCGcccgc                                                                                                         >  2:3299202/1‑146 (MQ=255)
                                            gTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCCCACCCAAACCCAAAACCACACGGCGCGGGGCCCCGAGGGCCCCGTATGTACTCCCCCACAATGAAAAAAAACCCCACACttt                                                                                                      >  2:1429870/1‑150 (MQ=255)
                                             tAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCtttt                                                                                                     <  2:1772545/150‑1 (MQ=255)
                                                 gtgTTGCCTGCGGAACTGGCTCCCATGGCGAATGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGTAGTGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTtc                                                                                                 <  1:3002365/148‑1 (MQ=255)
                                                 gagTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTtc                                                                                                 <  1:3798726/150‑1 (MQ=255)
                                                 gagTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACGCCCTCACTCTGAAACGACACCGCACTCTTTTTTtc                                                                                                 <  1:3007251/150‑1 (MQ=255)
                                                   gTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGGCCCCGCACCCAAAACCGCACCCGCAGGGAGAGGGGACCGGTGCGCGCGGTATGTACTCCCGCACACAGACACGGCACCCCACGCTGTTTTtatc                                                                                               >  2:2111599/1‑150 (MQ=255)
                                                   gTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCCGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTtctc                                                                                               <  1:822237/150‑1 (MQ=255)
                                                   gTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTtctc                                                                                               <  2:972755/150‑1 (MQ=255)
                                                   gTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTtctc                                                                                               <  2:2842934/150‑1 (MQ=255)
                                                    ttGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTcc                                                                                              <  1:1582459/150‑1 (MQ=255)
                                                    ttGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACAACCTAAACCTGTCCCTGAGGGTGTGGGGTCCGTTAGGGGCTTTATGTAATACCTCAAACTGAAACGACAACGCACTCTTTTTTTATcc                                                                                              >  2:924836/1‑150 (MQ=255)
                                                    ttGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCCCACCCGAACCCGCTCCCCCACGGAGAGGGGCCCGGTCGGCGCTGGAGGGACACCCGCACACGGCAACCACACCCCCCGCATTTTTTCGcc                                                                                              >  1:3755287/1‑150 (MQ=255)
                                                     tGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTccc                                                                                             <  2:2056443/150‑1 (MQ=255)
                                                       ccTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCGCAACCCAAACCACACCCGCACGGGGAGGGGGCCCGTCGGCGCTGGTTTGAATCCCCCACACGGTAACCACACCCCCCCCATTTTTtatcacgc                                                                                           >  2:2811832/1‑146 (MQ=255)
                                                       ccTGCGGCACTGGCTCCCATGGAGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTc                                                                                           <  2:682930/150‑1 (MQ=255)
                                                         tGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCCCCCTGACCCACTACCTCAGGGAGAGGGGGCCGTTCGGCGCTGTATGTACTCCCTCACTCGGTAAAGACACCGCTCTCTTTTTTTCTCCCTCGc                                                                                         >  2:2594358/1‑150 (MQ=255)
                                                             gCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCCCCCCCAAACCCACACCCGCAGGGCGAGGGGTCCGGTCGGCGCGGTATGTACTCCCGCACACGGAAACGACACCGCCCACATTTTTTATCCCGCGccccg                                                                                     >  2:517279/1‑149 (MQ=255)
                                                               aCTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCACACCCAAAACCACTCCCACAGGGCGTGGGGGCCGTTAGGCGCGGTATTTACGCCCCCAAACAGAAACGACACCCCACTATTTTTTTATACCTCGCCCCAcc                                                                                   >  2:627248/1‑150 (MQ=255)
                                                                  ggCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCCGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCggg                                                                                <  2:2000878/150‑1 (MQ=255)
                                                                  ggCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCggg                                                                                <  1:719950/150‑1 (MQ=255)
                                                                  ggCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCggg                                                                                <  2:1290545/150‑1 (MQ=255)
                                                                  ggCGCCCATGGCGAACGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTACCCCTCTCCCTCCGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCCCTCGGAAACGCCACCGCACTCTTTTTTTCTCCCTCGCCCCTCCggg                                                                                <  2:1282052/150‑1 (MQ=255)
                                                                    cTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGga                                                                              <  1:2043150/150‑1 (MQ=255)
                                                                    cTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCAACCGAAACCAGTCCCTGAGGGTGTGGGGTCCGTTTGGGGGGGTTTTTTATACCGAAATCTGTAAAGACACCGCACTCTTTTTTTTTGGCACGGCCCTGCggggg                                                                              >  2:465642/1‑149 (MQ=255)
                                                                     tCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCCCACCCAAACCCACTCCCACAGGGCGAGGGGCCCGGTCGGCGCGGTATGTGCTCCCACCCACTGAAACCACAACGCCCGCTTTTTTTCTACCCCGCCCCCCCgggggg                                                                             >  2:1918536/1‑148 (MQ=255)
                                                                      cccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGgaga                                                                            <  1:307644/150‑1 (MQ=255)
                                                                      cccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGgaga                                                                            <  1:582516/150‑1 (MQ=255)
                                                                      cccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGgaga                                                                            <  1:217077/150‑1 (MQ=255)
                                                                      cccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGgaga                                                                            <  1:216955/150‑1 (MQ=255)
                                                                      cccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGgaga                                                                            <  2:453709/150‑1 (MQ=255)
                                                                      cccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCTAACCCTCTCCCTCAGGGAGTGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCTCTCTTTTTTTCTCCCTCGCCCCTCCGTGgaga                                                                            <  1:216924/150‑1 (MQ=255)
                                                                        cATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAgg                                                                          <  2:3433752/150‑1 (MQ=255)
                                                                        cATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAgg                                                                          <  1:3654011/150‑1 (MQ=255)
                                                                        cATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAgg                                                                          <  1:1686890/150‑1 (MQ=255)
                                                                         aTGGCGAAAGGGAATGCGGCATTGTCGTTTGCGCCCCCTCACCCTCACCCTCTCCCTCCGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAggg                                                                         <  1:3864363/150‑1 (MQ=255)
                                                                         aTGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCTCCCCGGAGGGGCGAggg                                                                         <  2:1586200/150‑1 (MQ=255)
                                                                         aTGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAggg                                                                         <  1:3864327/150‑1 (MQ=255)
                                                                          tGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGc                                                                        <  2:79380/150‑1 (MQ=255)
                                                                            gCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCCCCCCCAAAACCCCTCCCGCCCGGGGCGGGGGCCCGTCGGCGCGGGAAGGTCTACCCCACACAGTAACCGCACCCCCCACATTTTTTGTCCCGCCCCCCGCCCGGGGGGGGGGCg                                                                      >  1:2817449/1‑150 (MQ=255)
                                                                              gAAAGGGATTGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCggg                                                                    <  1:1054689/150‑1 (MQ=255)
                                                                              gAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGTGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCggg                                                                    <  1:1047643/150‑1 (MQ=255)
                                                                              gAAAGGGAATGCGGCAGAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCggg                                                                    <  2:3464795/150‑1 (MQ=255)
                                                                                  gggAAGGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCCGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGa                                                                <  1:3084163/150‑1 (MQ=255)
                                                                                   ggcaTGCGGCAGAGTCGTTTGCGCCCCCTCCCCCTCCCCCTCTCCCTCCGGGAGAGGGGACCGGTCGGCGCTGTATGTACTCCCTCACTCTGAACCGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAg                                                               <  2:3530058/147‑1 (MQ=255)
                                                                                   ggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTTTATGTACTCCCTCACTCCGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAg                                                               <  1:183908/150‑1 (MQ=255)
                                                                                   ggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAg                                                               <  1:2372329/150‑1 (MQ=255)
                                                                                   ggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAg                                                               <  1:2372360/150‑1 (MQ=255)
                                                                                   ggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAg                                                               <  1:3256858/150‑1 (MQ=255)
                                                                                   ggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCCGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAg                                                               <  1:183904/150‑1 (MQ=255)
                                                                                   ggAATGCGGCAGAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCCGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCCCTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAg                                                               <  2:3530136/150‑1 (MQ=255)
                                                                                         cGGCATAGTCGTTTGCGCCCCCTCAGCCTTACCCTCTCCTGTGGGTGAGGGGAGCGTTGGGCGGTGTATTTTGTGCCTCACTCTGTAAAGACACCGCACTGTTTTTTTTTACCTCGCCCCTACGGGGGGAGGGGCGGGGTGTGGGGtaaa                                                        >  2:2708704/1‑150 (MQ=255)
                                                                                             tttgttttttGCGCCCCCACCCACTTACCCTCCCCTTGTGGGTGTGGGCACGTTGGGGGGATTTATTTACTCCCTCACTCTTAAACTACACCAAAATCTTTTTTTCTCCCTCGCCCCTCGGGGTTGTGGGCGGGGGTTTGGGTAAAAGGc                                                     <  2:2098910/143‑1 (MQ=255)
                                                                                                              cTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAAtt                                    >  2:3053070/1‑150 (MQ=255)
                                                                                                              cTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAAtt                                    >  1:3494985/1‑150 (MQ=255)
                                                                                                              cTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAAtt                                    >  2:443619/1‑150 (MQ=255)
                                                                                                                   ccTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGACAAGGCCGCACTGCTCACCAATTTTCTg                               >  2:1690669/1‑150 (MQ=255)
                                                                                                                   ccTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTg                               >  1:3254760/1‑150 (MQ=255)
                                                                                                                   ccTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTg                               >  1:3593563/1‑150 (MQ=255)
                                                                                                                   ccTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTg                               >  1:1155807/1‑150 (MQ=255)
                                                                                                                     tAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCt                             >  2:3553599/1‑150 (MQ=255)
                                                                                                                      tccccTCTCCCTGAGGGAGAGGGAACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTa                            >  2:817003/3‑150 (MQ=255)
                                                                                                                         ccTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATcc                         >  1:1186455/1‑150 (MQ=255)
                                                                                                                          ctctCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCt                        >  1:41037/1‑150 (MQ=255)
                                                                                                                           tctcCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGACAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTg                       >  2:1041088/1‑150 (MQ=255)
                                                                                                                           tctcCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTg                       >  2:3047595/1‑150 (MQ=255)
                                                                                                                                       agagGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGTCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTaa           >  2:3542029/1‑150 (MQ=255)
                                                                                                                                           gggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATATTCTGCTATCCTGCCCCCGCACTAAgggg       >  2:2890740/1‑150 (MQ=255)
                                                                                                                                                cGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGt  >  2:2385932/1‑150 (MQ=255)
                                                                                                                                                cGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGt  >  2:3531666/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
ATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGT  >  NC_000913/3780921‑3781214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: