| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,487,100 | G→T | V104F (GTC→TTC) | yhfK → | putative transporter, FUSC superfamily inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,487,100 | 0 | G | T | 98.4% | 212.4 / NA | 63 | V104F (GTC→TTC) | yhfK | putative transporter, FUSC superfamily inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (32/30); total (32/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATAAACGTTTTTTCAAACGCTTAATCATTGGTGCGTCGCTGTTTGCCACCTGTAGCTTGCTGACACAGCTACTACTGGCAAAAGATGTTCCCCTGCCCTTTTTGCTGACCGGATTAACGCTGGTACTTGGCGTCACTGCTGAGCTGGGGCCATTGCACGCAAAATTGCTTCCTGCATCGCTGCTCGCCGCCATTTTTACCCTCAGTTTGGCGGGATACATGCCGGTCTGGGAACCGTTGCTCATCTATGCGTTGGGCACTCTCTGGTA > NC_000913/3486969‑3487236 | aTAAACGTTTTTTCAAACGCTTAATCATTGGTGCGTCGCTGTTTGCCACCTGTAGCTTGCTGACACAGCTACTACTGGCAAAAGATGTTCCCCTGCCCTTTTTGCTGACCGGATTAACGCTGGTACTTGGCTTCActgct < 1:606076/140‑1 (MQ=255) aCGTTTTTTCAAACGCTTAATCATTGGTGCGTCGCTGTTTGCCACCTGTAGCTTGCTGACACAGCTACTACTGGCAAAAGATGTTCCCCTGCCCTTTTTGCTGACCGGATTAACGCTGGTACTTGGCTTCACTGCTGAGc < 1:1395804/140‑1 (MQ=255) cGTTTTTTCAAACGCTTAATCATTGGTGCGTCGCTGTTTGCCACCTGTAGCTTGCTGACACAGCTACTACTGGCAAAAGATGTTCCCCTGCCCTTTTTGCTGACCGGATTAACGCTGGTACTTGGCTTCACTGCTGAGCt < 2:968140/140‑1 (MQ=255) ttttttCAAACGCTTAATCATTGGTGCGTCGCTGTTTGCCACCTGTAGCTTGCTGACACAGCTACTACTGGCAAAAGATGTTCCCCTGCCCTTTTTGCTGACCGGATTAACGCTGGTACTTGGCTTCACTGCTGAGCTgg > 1:549730/1‑140 (MQ=255) aCGCTTAATCATTGGTGCGTCGCTGTTTGCCACCTGTAGCTTGCTGACACAGCTACTACTGGCAAAAGATGTTCCCCTGCCCTTTTTGCTGACCGGATTAACGCTGGTACTTGGCTTCACTGCTGAGCTGGGGCCATTGc > 1:1349555/1‑140 (MQ=255) cGCTTAATCATTGGTGCGTCGCTGTTTGCCACCTGTAGCTTGCTGACACAGCTACTACTGGCAAAAGATGTTCCCCTGCCCTTTTTGCTGACCGGATTAACGCTGGTACTTGGCTTCACTGCTGAGCTGGGGCCATTGCa < 1:1351041/140‑1 (MQ=255) cGCTTAATCATTGGTGCGTCGCTGTTTGCCACCTGTAGCTTGCTGACACAGCTACTACTGGCAAAAGATGTTCCCCTGCCCTTTTTGCTGACCGGATTAACGCTGGTACTTGGCTTCACTGCTGAGCTGGGGCCATTGCa < 1:1355017/140‑1 (MQ=255) tAATCATTGGTGCGTCGCTGTTTGCCACCTGTAGCTTGCTGACACAGCTACTACTGGCAAAAGATGTTCCCCTGCCCTTTTTGCTGACCGGATTAACGCTGGTACTTGGCTTCACTGCTGAGCTGGGGCCATTGCACGCa < 2:311083/140‑1 (MQ=255) tAATCATTGGTGCGTCGCTGTTTGCCACCTGTAGCTTGCTGACACAGCTACTACTGGCAAAAGATGTTCCCCTGCCCTTTTTGCTGACCGGATTAACGCTGGTACTTGGCTTCACTGCTGAGCTGGGGCCATTGCACGCa < 2:148124/140‑1 (MQ=255) tGGTGCGTCGCTGTTTGCCACCTGTAGCTTGCTGACACAGCTACTACTGGCAAAAGATGTTCCCCTGCCCTTTTTGCTGATCGGATTAACGCTGGTACTTGGCTTCACTGCTGAGCTGGGGCCATTGCACGCAAAATTGc > 2:715675/1‑140 (MQ=255) tGGTGCGTCGCTGTTTGCCACCTGTAGCTTGCTGACACAGCTACTACTGGCAAAAGATGTTCCCCTGCCCTTTTTGCTGACCGGATTAACGCTGGTACTTGGCTTCACTGCTGAGCTGGGGCCATTGCACGCAAAATTGc > 2:944138/1‑140 (MQ=255) tGGTGCGTCGCTGTTTGCCACCTGTAGCTTGCTGACACAGCTACTACTGGCAAAAGATGTTCCCCTGCCCTTTTTGCTGACCGGATTAACGCTGGTACTTGGCTTCACTGCTGAGCTGGGGCCATTGCACGCAAAATTGc > 1:1283630/1‑140 (MQ=255) tGGTGCGTCGCTGTTTGCCACCTGTAGCTTGATGACACAGCTACTACTGGCAAAAGATGTTCCCATGCCCTTTTTGCTGACCGGATTAACGCTGGTCCTTGGATTGACTGCTGAGCGGGGGCCATTGCACGCAAAATTGc > 2:1401250/1‑140 (MQ=255) ggTGCGTCGCTGTTTGCCACCTGTAGCTTGCTGACACAGCTACTACTGGCAAAAGATGTTCCCCTGCCCTTTTTGCTGACCGGATTAACGCTGGTACTTGGCTTCACTGCTGAGCTGGGGCCATTGCACGCAAAATTGCt < 1:304960/140‑1 (MQ=255) ggTGCGTCGCTGTTTGCCACCTGTAGCTTGCTGACACAGCTACTACTGGCAAAAGATGTTCCCCTGCCCTTTTTGCTGACCGGATTAACGCTGGTACTTGGCTTCACTGCTGAGCTGGGGCCATTGCACGCAAAATTGCt < 1:200403/140‑1 (MQ=255) ggTGCGTCGCTGTTTGCCACCTGTAGCTTGCTGACACAGCTACTACTGGCAAAAGATGTTCCCCTGCCCTTTTTGCTGACCGGATTAACGCTGGTACTTGGCTTCACTGCTGAGCTGGGGCCATTGCACGCAAAATTGCt < 2:640598/140‑1 (MQ=255) cGTCGCTGTTTGCCACCTGTAGCTTGCTGACACAGCTACTACTGGCAAAAGATGTTCCCCTGCCCTTTTTGCTGACCGGATTAACGCTGGTACTTGGCTTCACTGCTGAGCTGGGGCCATTGCACGCAAAATTGCTTCCt > 1:781645/1‑140 (MQ=255) gTCGCTGTTTGCCACCTGTAGCTTGCTGACACAGCTACTACTGGCAAAAGATGTTCCCCTGCCCTTTTTGCTGACCGGATTAACGCTGGTACTTGGCTTCACTGCTGAGCTGGGGCCATTGCACGCAAAATTGCTTCCTg < 1:788742/140‑1 (MQ=255) cTGTTTGCCACCTGTAGCTTGCTGACACAGCTACTACTGGCAAAAGATGTTCCCCTGCCCTTTTTGCTGACCGGATTAACGATGGAACTTGGCTTCACTGCTGAGCTGGGGCCATTGCACGCAAAATTGCTTCCTGCATc > 2:549019/1‑140 (MQ=255) tGCCACCTGTAGCTTGCTGACACAGCTACTACTGGCAAAAGATGTTCCCCTGCCCTTTTTGCTGACCGGATTAACGCTGGTACTTGGCTTCACTGCTGTGCTGGGGCCATTGCACGCCATATTGCTTCCTGCATCgctgc > 2:1425442/1‑140 (MQ=255) gCTGACACAGCTACTACTGGCAAAAGTTGTTCGCCTGCCCTTTTTGCTGACCGGATTATCGGTGGTAGTTGGCTTCACTGGTGAGCTGGGGCCATTGCACGCAATATTGCTTCCTGCATCGCTGCTCGCCGCCATTTTTa > 2:227084/1‑140 (MQ=255) gCTGACACAGCTACTACTGGCAAAAGATGTTCCCCTGCCCTTTTTGCTGTCCGGATTATCGCTGGTACTTGGCTTCACTGCTGAGCTGGGGCCATTGCACGCAAAATTGCTTCCTGCATCGCTGCTCGCCGCCATGTTTa > 2:116570/1‑140 (MQ=255) gCTGACACAGCTACTACTGGCAAAAGATGTTCCCCTGCCCTGTTTGCTGACCGGATTAACGCTGGTACTTGGCTTCACTGCTGAGCTGGGGCCATTGCACGCAAAATTGCTTCCTGCATCGCTGCTCGCCGCCATTTTTa > 2:698926/1‑140 (MQ=255) gCTGACACAGCTACTACTGGCAAAAGATGTTCCCCAGCCCTTTTTGCTGACCGGATTAACGCTGGTACTTGGCTTCACAGCTGAGCTGGGGCCATTGCACGCAAAATTGCTTCCTGCATCGCTGCTCGCCGCCATTTTTa > 2:461906/1‑140 (MQ=255) gACACAGCTACTACTGGCAAAAGATGTTCCCCTGCCCTTTTTGCTGACCGGATTAACGCTGGTACTTGGCTTCACTGCTGAGCTGGGGCCATTGCACGCAAAATTGCTTCCTGCATCGCTGCTCGCCGCCATTTTTAccc < 1:254230/140‑1 (MQ=255) acacAGCTACTACTGGCAAAAGATGTTCCCCTGCCCTTCTTGCTGACCGGATTAACGCTGGTACTTGGCTTCACTGCTGAGCTGGGGCCATTGCACGCAAAATTGCTTCCTGCATCGCTGCTCGCCGCCATTTTTACCCt < 1:1392250/140‑1 (MQ=255) gCTACTACTGGCAAAAGATGTTCCCCTGCCCTTTTTGCTGACCGGATTAACGCTGGTACTTGGCTTCACTGCTGAGCTGGGGCCATTGCACGCAAAATTGCTTCCTGCATCGCTGCTCGCCGCCATTTTTACCCTCAGtt < 1:582619/140‑1 (MQ=255) ctactGGGAAAAGATGTTCCCCTGCCCTTTTTGGTGACCTGATTAACGCTGGTACTTGGCTTCACTGCTGAGCTGGGGCCATTGCACGCAAAATTGCTTCCTGCATCGCTGCTCGCCGCCATTTTTACCCTCAGTTTGGc < 2:374520/140‑1 (MQ=255) ctactGGCAAAAGATGTTCCCCTGCCCTTTTTGCTGACCGGATTAACGCTGGTACTTGGCTTCACTGCTGAGCTGGGTCCATTGCACGCAAAATTGCTTCCAGCATCGCTGCTCGCCGCCATTTTTACCCTCAGTTTGGc < 2:216494/140‑1 (MQ=255) ctactGGCAAAAGATGTTCCCCTGCCCTTTTTGCTGACCGGATTAACGCTGGTACTTGGCTTCACTGCTGAGCTGGGGCCATTGCACGCAAAATTGCTTCCTGCATCGCTGCTCGCCGCCATTTTTACCCTCAGTTTGGc < 2:279534/140‑1 (MQ=255) ctGGCAAAAGATGTTCCCCTGCCCTTTTTGCTGACCGGATTAACGCTGGTACTTGGCTTCACTGCTGAGCTGGGGCCATTGCACGCAAAATTGCTTCCTGCATCGCTGCTCGCCGCCATTTTTACCCTCAGTTTGGCggg > 2:1446422/1‑140 (MQ=255) ggCAAAAGATGTTCCCCTGCCCTTTTTGCTGACCGGATTAACGCTGGTACTTGGCTTCACTGCTGAGCTGGGGCCATTGCACGCAAAATTGCTTCCTGCATCGCTGCTCGCCGCCATTTTTACCCTCAGTTTGGCGGGAt < 1:295112/140‑1 (MQ=255) cAAAAGATGTTCCCCTGCCCTTTTTGCTGACCGGATTAACGCTGGTACTTGGCTTCACTGCTGAGCTGGGGCCATTGCACGCAAAATTGCTTCCTGCATCGCTGCTCGCCGCCATTTTTACCCTCAGTTTGGCGGGATAc > 2:989694/1‑140 (MQ=255) cAAAAGATGTTCCCCTGCCCTTTTTGCTGACCGGATTAACGCTGGTACTTGGCTTCACTGCTGAGCTGGGGCCATTGCACGCAAAATTGCTTCCTGCATCGCTGCTCGCCGCCATTTTTACCCTCAGTTTGGCGGGATAc > 2:989863/1‑140 (MQ=255) aaGATGTTCCCCTGCCCTTTTTGCTGACCGGATTAACGCTGGTACTTGGCTTCACTGCTGAGCTGGGGCCATTGCACGCAAAATTGCTTCCTGCATCGCTGCTCGCCGCCATTTTTACCCTCAGTTTGGCGGGATACATg > 1:875847/1‑140 (MQ=255) aaGATGTTCCCCTGCCCTTTTTGCTGACCGGATTAACGCTGGTACTTGGCTTCACTGCTGAGCTGGGGCCATTGCACGCAAAATTGCTTCCTGCATCGCTGCTCGCCGCCATTTTTACCCTCAGTTTGGCGGGATACATg > 2:675092/1‑140 (MQ=255) aaGATGTTCCCCTGCCCTTTTTGCTGACCGGATTAACGCTGGTACTTGGCTTCACTGCTGAGCTGGGGCCATTGCACGCAAAATTGCTTCCTGCATCGCTGCTCGCCGCCATTTTTACCCTCAGTTTGGCGGGATACATg < 1:1607791/140‑1 (MQ=255) aaGATGTTCCCCTGCCCTTTTTGCTGACCGGATTAACGCTGGTACTTGGCTTCACTGCTGAGCTGGGGCCATTGCACGCAAAATTGCTTCCTGCATCGCTGCTCGCCGCCATTTTTACCCTCAGTTTGGCGGGATACATg > 2:679003/1‑140 (MQ=255) aaGATGTTCCCCTGCCCTTTTTGCTGACCGGATTAACGCTGGTACTTGGCTTCACTGCTGAGCTGGGGCCATTGCACGCAAAATTGCTTCCTGCATCGCTGCTCGCCGCCATTTTTACCCTCAGTTTGGCGGGATACATg < 1:1235896/140‑1 (MQ=255) aaGATGTTCCCCTGCCCTTTTTGCTGACCGGATTAACGCTGGTACTTGGCTTCACTGCTGAGCTGGGGCCATTGCACGCAAAATTGCTTCCTGCATCGCTGCTCGCCGCCATTTTTACCCTCAGTTTGGCGGGATACATg < 1:1235822/140‑1 (MQ=255) aaGATGTTCCCCTGCCCTTTATGCTGACCGGATTAACGCTGGTACTTGGCTTCACTGCTGAGCTGGGGCCATTGCACGCAAAATTGCTTCCTGCATCGCTGCTCGCCGCCATTTTTACCCTCAGTTTGGCGGGATACATg > 1:876154/1‑140 (MQ=255) ttCCCCTGCCCTTTTTGCTGACCGGATTAACGCTGGTACTTGGCTTCACTGCTGAGCTGGGGCCATTGCACGCAAAATTGCTTCCTGCATCGCTGCTCGCCGCCATTTTTACCCTCAGTTTGGCGGGATACATGCCGGTc > 1:1019902/1‑140 (MQ=255) ttCCCCTGCCCTTTTTGCTGACCGGATTAACGCTGGTACTTGGCTTCACTGCTGAGCTGGGGCCATTGCACGCAAAATTGCTTCCTGCATCGCTGCTCGCCGCCATTTTTACCCTCAGTTTGGCGGGATACATGCCGGTc > 1:1249428/1‑140 (MQ=255) cTTTTTGCTGACCGGATTAACGCTGGTACTTGGCTTCACTGCTGAGCTGGGGCCATTGCACGCAAAATTGCTTCCTGCATCGCTGCTCGCCGCCATTTTTACCCTCAGTTTGGCGGGATACATGCCGGTCTGGGAACCGt > 2:207542/1‑140 (MQ=255) ttttGCTGACCGGATTAACGCTGGTACTTGGCGTCACTGCTGAGCTGGGGCCATTGCACGCAAAATTGCTTCCTGCATCGCTGCTCGCCGCCATTTTTACCCTCAGTTTGGCGGGATACATGCCGGTCTGGGAACCGTTg < 2:700513/140‑1 (MQ=255) tttGCTGACCGGATTAACGCTGGTACTTGGCTTCACTGCTGAGCTGGGGCCATTGCACGCAAAATTGCTTCCTGCATCGCTGCTCGCCGCCATTTTTACCCTCAGTTTGGCGGGATACATGCCGGTCTGGGAACCGTTGc > 1:1034021/1‑140 (MQ=255) ttGCTGACCGGATTAACGCTGGTACTTGGCTTCACTGCTGAGCTGGGGCCATTGCACGCAAAATTGCTTCCTGCATCGCTGCTCGCCGCCATTTTTACCCTCAGTTTGGCGGGATACATGCCGGTCTGGGAACCGTTGCt < 2:25637/140‑1 (MQ=255) ttGCTGACCGGATTAACGCTGGTACTTGGCTTCACTGCTGAGCTGGGGCCATTGCACGCAAAATTGCTTCCTGCATCGCTGCTCGCCGCCATTTTTACCCTCAGTTTGGCGGGATACATGCCGGTCTGGGAACCGTTGCt < 2:386745/140‑1 (MQ=255) ttGCTGACCGGATTAACGCTGGTACTTGGCTTCACTGCTGAGCTGGGGCCATTGCACGCAAAATTGCTTCCTGCATCGCTGCTCGCCGCCATTTTTACCCTCAGTTTGGCGGGATACATGCCGGTCTGGGAACCGTTGCt < 1:1446422/140‑1 (MQ=255) ttGCTGACCGGATTAACGCTGGTACTTGGCTTCACTGCTGAGCTGGGGCCATTGCACGCAAAATTGCGTCCTGCATCGCTGCTCGCCGCCATTTTTACCCTCAGTTTGGCGGGATACATGCCGGTCTGGGAACCGTTGCt < 2:1598874/140‑1 (MQ=255) tGACCGGATTAACGCTGGTACTTGGCTTCACTGCTGAGCTGGGGCCATTGCACGCAAAATTGCTTCCTGCATCGCTGCTCGCCGCCATTTTTACCCTCAGTTTGGCGGGATACATGCCGGTCTGGGAACCGTTGCTCATc > 1:797753/1‑140 (MQ=255) gACCGGATTAACGCTGGTACTTGGCTTCACTGCTGAGCTGGGGCCATTGCACGCAAAATTGCTTCCTGCATCGCTGCTCGCCGCCATTTTTACCCTCAGTTTGGCGGGATACATGCCGGTCTGGGAACCGTTGCTCATCt < 1:1083801/140‑1 (MQ=255) gACCGGATTAACGCTGGTACTTGGCTTCACTGCTGAGCTGGGGCCATTGCACGCAAAATTGCTTCCTGCATCGCTGCTCGCCGCCATTTTTACCCTCAGTTTGGCGGGATACATGCCGGTCTGGGAACCGTTGCTCATCt > 1:1565361/1‑140 (MQ=255) aTTAACGCTGGTACTTGGCTTCACTGCTGAGCTGGGGCCATTGCACGCAAAATTGCTTCCTGCATCGCTGCTCGCCGCCATTTTTACCCTCAGTTTGGCGGGATACATGCCGGTCTGGGAACCGTTGCTCATCTATGCGt < 2:626753/140‑1 (MQ=255) aaCGCTGGTACTTGGCTTCACTGCTGAGCTGGGGCCATTGCACGCAAAATTGCTTCCTGCATCGCTGCTCGCCGCCATTTTTACCCTCAGTTTGGCGGGATACATGCCGGTCTGGGAACCGTTGCTCATCTATGCGTTgg < 2:1510207/140‑1 (MQ=255) gTACTTGGCTTCACTGCTGAGCTGGGGCCATTGCACGCAAAATTGCTTCCTGCATCGCTGCTCGCCGCTATTTTTACCCTCAGTTTGGCGGGATACATGCCGGTCTGGGAACCGTTGCTCATCTATGCGTTGGGCActct > 1:1007651/1‑140 (MQ=255) gTACTTGGCTTCACTGCTGAGCTGGGGCCATTGCACGCAAAATTGCTTCCTGCATCGCTGCTCGCCGCCATTTTTACCCTCAGTTTGGCGGGATACATGCCGGTCTGGGAACCGTTGCTCATCTATGCGTTGGGCActct > 1:85984/1‑140 (MQ=255) gTACTTGGCTTCACTGCTGAGCTGGGGCCATTGCACGCAAAATTGCTTCCTGCATCGCTGCTCGCCGCCATTTTTACCCTCAGTTTGGCGGGATACATGCCGGTCTGGGAACCGTTGCTCATCTATGCGTTGGGCActct > 1:144134/1‑140 (MQ=255) gTACTTGGCTTCACTGCTGAGCTGGGGCCATTGCACGCAAAATTGCTTCCTGCATCGCTGCTCGCCGCCATTTTTACCCTCAGTTTGGCGGGATACATGCCGGTCTGGGAACCGTTGCTCATCTATGCGTTGGGCActct < 2:1019902/140‑1 (MQ=255) gTACTTGGCTTCACTGCTGAGCTGGGGCCATTGCACGCAAAATTGCTTCCTGCATCGCTGCTCGCCGCCATTTTTACCCTCAGTTTGGCGGGATACATGCCGGTCTGGGAACCGTTGCTCATCTATGCGTTGGGCActct > 2:1277691/1‑140 (MQ=255) gTACTTGGCTTCACTGCTGAGCTGGGGCCATTGCACGCAAAATTGCTTCCTGCATCGCTGCTCGCCGCCATTTTTACCCTCAGTTTGGCGGGATACATGCCGGTCTGGGAACCGTTGCTCATCTATGCGTTGGGCActct > 2:253352/1‑140 (MQ=255) tGGCTTCACTGCTGAGCTGGGGCCATTGCACGCAAAATTGCTTCCTGCATCGCTGCTCGCCGCCATTTTTACCCTCAGTTTGGCGGGATACATGCCGGTCTGGGAACCGTTGCTCATCTATGCGTTGGGCACTCTCTGGt < 1:602410/140‑1 (MQ=255) ggCTTCACTGCTGAGCTGGGGCCATTGCACGCAAAATTGCTTCCTGCATCGCTGCTCGCCGCCATTTTTACCCTCAGTTTGGCGGGATACATGCCGGTCTGGGAACCGTTGCTCATCTATGCGTTGGGCACTCTCTGGTa > 2:189832/1‑140 (MQ=255) | ATAAACGTTTTTTCAAACGCTTAATCATTGGTGCGTCGCTGTTTGCCACCTGTAGCTTGCTGACACAGCTACTACTGGCAAAAGATGTTCCCCTGCCCTTTTTGCTGACCGGATTAACGCTGGTACTTGGCGTCACTGCTGAGCTGGGGCCATTGCACGCAAAATTGCTTCCTGCATCGCTGCTCGCCGCCATTTTTACCCTCAGTTTGGCGGGATACATGCCGGTCTGGGAACCGTTGCTCATCTATGCGTTGGGCACTCTCTGGTA > NC_000913/3486969‑3487236 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |